Skip to main content

Official Journal of the Japan Wood Research Society

  • Note
  • Published:

Rapid extraction of epidermis chlorophyll of moso bamboo (Phyllostachys pubescens) culm using ultrasonics

Abstract

The feasibility of applying ultrasonics to the chlorophyll extraction of bamboo culm was evaluated to establish an efficient reliable method for chlorophyll extraction from the epidermis of bamboo culm. The results demonstrate that extraction of epidermis chlorophyll from bamboo culm with acetone by ultrasonics for 3 min is a rapid, reliable method.

References

  1. Chang ST, Lee HL (1996) Protection of the green color of Moso bamboo (Phyllostachys edulis) culms and its colorfastness after treatment. Mokuzai Gakkaishi 42:392–396

    CAS  Google Scholar 

  2. Chang ST (1997) Comparison of the green color fastness of Ma (Dendrocalamus spp.) bamboo culms treated with inorganic salts. Mokuzai Gakkaishi 43:487–492

    CAS  Google Scholar 

  3. Schanderl SH, Marsh GL, Chichester CO (1965) Color reversion in processed vegetables. 1. Studies on regreened pea purees. J Food Sci 30:312–316

    Article  Google Scholar 

  4. Schanderl SH, Marsh GL, Chichester CO (1965) Color reversion in processed vegetables. II. Model system studies. J Food Sci 30:317–324

    Article  Google Scholar 

  5. Kowalewska G, Falkowski L, Hoffmann SK, Szczepaniak LS (1987) Replacement of magnesium by copper. 11. In the chlorophyll porphyrin ring of planktonic algae. Acta Physiol Plantarum 9:43–52

    CAS  Google Scholar 

  6. Mackinney G (1941) Absorption of light by chlorophyll solutions. J Biol Chem 140:315–322

    CAS  Google Scholar 

  7. Shoaf TW, Lium BW (1976) Improved extraction of chlorophyll a and b from algae using dimethyl sulphoxide. Limnol Oceanogr 21:926–928

    Article  CAS  Google Scholar 

  8. Moran R, Porath D (1980) Chlorophyll determination in intact tissues using N,N-dimethylformamide. Plant Physiol 65:478–485

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Inskeep WP, Bloom PR (1985) Extinction coefficients of chlorophyll a and b in N,N-dimethylformamide and 80% acetone. Plant Physiol 77:483–485

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Barnes JD, Balaguer L, Manrique E, Elvira S, Davison AW (1992) A reappraisal of the use of DMSO for the extraction and determination of chlorophylls a and b in lichens and higher plants. Environ Exp Bot 32:85–100

    Article  CAS  Google Scholar 

  11. Wegener G, Fengel D (1977) Studies on milled wood lignins from spruce. 1. Composition and molecular properties. Wood Sci Technol 11:133–145

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chang, ST., Wang, SY. & Wu, JH. Rapid extraction of epidermis chlorophyll of moso bamboo (Phyllostachys pubescens) culm using ultrasonics. J Wood Sci 44, 78–80 (1998). https://doi.org/10.1007/BF00521879

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00521879

Key words Bamboo