Skip to main content

Advertisement

Official Journal of the Japan Wood Research Society

Journal of Wood Science Cover Image

Rapid extraction of epidermis chlorophyll of moso bamboo (Phyllostachys pubescens) culm using ultrasonics

Article metrics

  • 190 Accesses

  • 9 Citations

Abstract

The feasibility of applying ultrasonics to the chlorophyll extraction of bamboo culm was evaluated to establish an efficient reliable method for chlorophyll extraction from the epidermis of bamboo culm. The results demonstrate that extraction of epidermis chlorophyll from bamboo culm with acetone by ultrasonics for 3 min is a rapid, reliable method.

References

  1. 1.

    Chang ST, Lee HL (1996) Protection of the green color of Moso bamboo (Phyllostachys edulis) culms and its colorfastness after treatment. Mokuzai Gakkaishi 42:392–396

  2. 2.

    Chang ST (1997) Comparison of the green color fastness of Ma (Dendrocalamus spp.) bamboo culms treated with inorganic salts. Mokuzai Gakkaishi 43:487–492

  3. 3.

    Schanderl SH, Marsh GL, Chichester CO (1965) Color reversion in processed vegetables. 1. Studies on regreened pea purees. J Food Sci 30:312–316

  4. 4.

    Schanderl SH, Marsh GL, Chichester CO (1965) Color reversion in processed vegetables. II. Model system studies. J Food Sci 30:317–324

  5. 5.

    Kowalewska G, Falkowski L, Hoffmann SK, Szczepaniak LS (1987) Replacement of magnesium by copper. 11. In the chlorophyll porphyrin ring of planktonic algae. Acta Physiol Plantarum 9:43–52

  6. 6.

    Mackinney G (1941) Absorption of light by chlorophyll solutions. J Biol Chem 140:315–322

  7. 7.

    Shoaf TW, Lium BW (1976) Improved extraction of chlorophyll a and b from algae using dimethyl sulphoxide. Limnol Oceanogr 21:926–928

  8. 8.

    Moran R, Porath D (1980) Chlorophyll determination in intact tissues using N,N-dimethylformamide. Plant Physiol 65:478–485

  9. 9.

    Inskeep WP, Bloom PR (1985) Extinction coefficients of chlorophyll a and b in N,N-dimethylformamide and 80% acetone. Plant Physiol 77:483–485

  10. 10.

    Barnes JD, Balaguer L, Manrique E, Elvira S, Davison AW (1992) A reappraisal of the use of DMSO for the extraction and determination of chlorophylls a and b in lichens and higher plants. Environ Exp Bot 32:85–100

  11. 11.

    Wegener G, Fengel D (1977) Studies on milled wood lignins from spruce. 1. Composition and molecular properties. Wood Sci Technol 11:133–145

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Chang, S., Wang, S. & Wu, J. Rapid extraction of epidermis chlorophyll of moso bamboo (Phyllostachys pubescens) culm using ultrasonics. J Wood Sci 44, 78–80 (1998) doi:10.1007/BF00521879

Download citation

Key words Bamboo

  • Phyllostachys pubescens
  • Chlorophyll
  • Extraction
  • Ultrasonics