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Journal of Wood Science

Official Journal of the Japan Wood Research Society

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Changes in carbohydrase activities during vegetative growth and development of fruit-bodies of Hypsizygus marmoreus grown in sawdust-based culture

Journal of Wood Science volume 44, pages 234–236 (1998)Cite this article

Abstract

Carbohydraseproduction of Hypsizygus marmoreus cultured on sawdust rice bran medium by bottle cultivation was investigated to elucidate the carbohydrase utilized as the growth substrate for the fruit-body formation of this fungus. Among the extracellular enzymes assayed, xylanase showed the highest activity. This activity greatly increased during the end of vegetative mycelial growth and fruit-body formation. Among the cellulases. CM-cellulase showed higher activity than avicelase. The activities of β-1,3-glucanase, amylase, and chitinase were low. Among the intracellular enzymes, both xylanase and amylase showed higher activity levels than the other carbohydrases. In contrast, β-1,3-glucanase, avicelase, and chitinase activities in mycelia were considerably lower. These results suggest that xylanase, CM-cellulase, and amylase play an important role in mycelial maturation and fruit-body growth of H. marmoreus.

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Authors and Affiliations

  1. Laboratory of Food Microbiology, Faculty of Agriculture, Kinki University, 631-8505, Nara, Japan

    Takao Terashita, Ryousuke Murao, Kentaro Yoshikawa & Jiko Shishiyama

Authors
  1. Takao Terashita
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  2. Ryousuke Murao
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  3. Kentaro Yoshikawa
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  4. Jiko Shishiyama
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Terashita, T., Murao, R., Yoshikawa, K. et al. Changes in carbohydrase activities during vegetative growth and development of fruit-bodies of Hypsizygus marmoreus grown in sawdust-based culture. J Wood Sci 44, 234–236 (1998). https://doi.org/10.1007/BF00521969

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  • DOI: https://doi.org/10.1007/BF00521969

Key words

  • Hypsizygus marmoreus
  • Carbohydrase
  • Mushroom fungi
  • Enzyme production
  • Fruit-body formation