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Journal of Wood Science

Official Journal of the Japan Wood Research Society

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Antioxidative activity of tree phenolic constituents 1: Radical-capturing reaction of flavon-3-ols with radical initiator

Journal of Wood Science volume 45, pages 53–63 (1999)Cite this article

Abstract

The present work was undertaken from the standpoint of radical-capturing ability with regard to the antioxidative ability of flavonoids, especially flavonols distributed widely in woody plants. In regard to the flavonols, six methyl derivatives were initially prepared from quercetin and its litinoside. Their radical-capturing constants were determined strictly by the stopped-flow spectroscopic method. It was proved that the radical-capturing ability of quercetin mainly involves the vicinal C3. and C4, hydroxyl groups and the C3 hydroxyl group. To clarify the reaction mechanism begun at the C3 hydroxyl group of quercetin, 5,7,3′,4′-tetramethylquercetin (TMQ), flavon-3-ol (F30) and so on were treated with 2,2′-azo-bis-(2,4-dimethylvaleronitrile) (AMVN). Six products (1–6) containing one depside and its two hydrolytic products, two valeronitrile adducts, and others were isolated from the reaction mixture of TMQ and their structures determined by instrumental analyses. Similarly, F30 gave four products, 7–10, which corresponded to the above products 1–3 and 5 (one depside, its two hydrolytic products, and one adduct), respectively. 3,5,7,3′,4′-Pentamethylquercetin (PMQ) and flavon-3-O-methylate (F3M) gave no products. The quantitative change of the products with reaction time was determined spectroscopically. An initial reaction pathway for the radical-capturing reaction of flavon-3-ols with AMVN was proposed based on the products and their amounts. The main route — formation of depside and its hydrolytic products via ketohydroperoxide (3″) or ketohydroperoxy radical (4″) - was similar to that of the oxidation reaction of quercetin with quercetinase and light.

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Author notes

  1. Tetsuya Kyogoku

    Present address: Ohtani Paint Mfg. Co., 537-0021, Osaka, Japan

  2. Shin-ichiro Kawase

    Present address: Research Institute for Food Science, Kyoto University, 611-0011, Uji, Japan

Authors and Affiliations

  1. Faculty of Agriculture, Gifu University, 501-1193, Gifu, Japan

    Hideo Ohashi, Tetsuya Kyogoku & Takahiro Ishikawa

  2. Ohtani Paint Mfg. Co., 537-0021, Osaka, Japan

    Shin-ichiro Kawase & Shingo Kawai

Authors
  1. Hideo Ohashi
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  2. Tetsuya Kyogoku
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  3. Takahiro Ishikawa
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  4. Shin-ichiro Kawase
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  5. Shingo Kawai
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Additional information

Part of this paper was presented at the 46th and 47th annual meetings of the Japan Wood Research Society, Kumamoto and Kouchi, April 1996 and 1997

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Ohashi, H., Kyogoku, T., Ishikawa, T. et al. Antioxidative activity of tree phenolic constituents 1: Radical-capturing reaction of flavon-3-ols with radical initiator. J Wood Sci 45, 53–63 (1999). https://doi.org/10.1007/BF00579524

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  • DOI: https://doi.org/10.1007/BF00579524

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