Skip to main content


Official Journal of the Japan Wood Research Society

Journal of Wood Science Cover Image

Structural changes of residual lignin in softwood kraft pulp treated with manganese peroxidase


Structural changes of residual lignin in unbleached softwood kraft pulp (SWKP) during manganese peroxidase (MnP) treatment were investigated to obtain some understanding of the biobleaching action of SWKP with MnP treatment. Alkaline-extracted lignin from darkened SWKP by MnP showed more intense color and contained moreo-quinone than that from control SWKP. However, no difference in the conjugatedα-carbonyl was observed between the lignins from MnP-treated and control SWKP. The nitrobenzene oxidation analysis revealed that oxidative condensation of non-condensed lignin in SWKP occurs during an early stage of MnP treatment. These observations were supported by the model experiment in which the lignin prepared from control SWKP was subjected to MnP treatments three times, and the changes of color and functional groups in the lignin were determined after each treatment. These results suggested that an increase ino-quinone and the condensation reaction of non-condensed lignin in SWKP are responsible for the characteristic darkening of SWKP during MnP treatment. It was also ascertained that darkened lignin was degraded and brightened by repeated MnP treatments.


  1. 1.

    Ehara K, Tsutsumi Y, Nishida T (1997) Biobleaching of softwood and hardwood kraft pulp with manganese peroxidase. Mokuzai Gakkaishi 43:861–868

  2. 2.

    Reid ID (1990) Biological bleaching of softwood kraft pulp with the fungusTrametes (Coriolus) versicolor. Tappi J 73(8):149–153

  3. 3.

    Reid ID, Paice MG (1994) Effect of residual lignin type and amount on bleaching of kraft pulp byTrametes versicolor. Appl Environ Microbiol 60:1395–1400

  4. 4.

    Katagiri N, Tsutsumi Y, Nishida T (1997) Biobleaching of softwood kraft pulp by white-rot fungi and its related enzymes. Mokuzai Gakkaishi 43:678–685

  5. 5.

    Arbeloa M, Leseleuc J, Goma G, Pommier JC (1992) An evaluation of the potential of lignin peroxidases to improve pulps. Tappi 175(3):215–221

  6. 6.

    Paice MG, Bourbonnais R, Reid ID (1995) Bleaching kraft pulps with oxidative enzymes and alkaline hydrogen peroxide. Tappi J 78(9):161–169

  7. 7.

    Fukui S (1982) Phenol sulfuric acid method (in Japanese). In: Uriya I, Shimura K, Nakamura M, Hunatsu M (eds) Determination of reducing sugar. Gakkai Shuppan. Tokyo, pp 45–47

  8. 8.

    Meshitsuka G (1990) Lignin chemistry (in Japanese). In: Usuda M, Mizumachi H, Iiyama K, Morohoshi N, Yamaguchi A (eds) Mokuzaikagaku jikkensyo II. Chugai Sangyo, Tokyo, pp 196–197

  9. 9.

    Adler E, Marton J (1959) Zur Kenntnis der carbonylgruppen im lignin. Acta Chem Scand 13:75–96

  10. 10.

    Lebo SE, Lonsky WFW, McDonough TJ, Medvecz PJ, Dimmel DR (1990) The occurrence and light-induced formation of orthoquinoid lignin structures in white spruce refiner mechanical pulp. J Pulp Paper Sci 16(5):7139–7143

  11. 11.

    Wariishi H, Valli K, Gold MH (1989) Oxidative cleavage of phenolic diarylpropane lignin model dimer by manganese peroxidase fromPhanerochaete chrysosporium. Biochemistry 28:6017–6023

  12. 12.

    Tuor U, Wariishi H, Schoemaker HE, Gold MH (1992) Oxidation of phenolic arylglycerolβ-aryl ether lignin model compounds by manganese peroxidase fromPhanerochaete chrysosporium. Biochemistry 31:4986–4995

  13. 13.

    Wariishi H, Valli K, Gold MH (1991) In vitro deporimerization of lignin manganese peroxidase ofPhanerochaete chrysosporium. Biochem Biophys Res Commun 176:269–275

Download references

Author information

Correspondence to Tomoaki Nishida.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Ehara, K., Tsutsumi, Y. & Nishida, T. Structural changes of residual lignin in softwood kraft pulp treated with manganese peroxidase. J Wood Sci 44, 327–331 (1998).

Download citation

Key words

  • Kraft pulp
  • Residual lignin
  • Manganese peroxidase
  • Functional group
  • Alkaline nitrobenzene oxidation