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Analysis of the electrophoretic karyotype ofFlammulina velutipes

Abstract

The karyotype ofFlammulina velutipes (Curt.: Fr.) Sing. was analyzed electrophoretically using contourclamped homogeneous electric field gel electrophoresis and hybridization with DNA probes. The chromosomal DNA from the monokaryon (Fv-4K) and the dikaryon (Fv-4) were resolved into six and eight bands, respectively. The sizes of the chromosomes ranged from 1.9 to 6.0 megabase (Mb) pairs. Each of the separated bands of chromosomal DNA was identified by use of five cloned probes. The number of these chromosomes was estimated to be 6 and 12, respectively; and the size of the entire genome was estimated to be about 20.1 and 38.6Mb, respectively. From a comparison of the hybridization patterns, the existence of allelic chromosomes of different sizes was deduced in the Fv-4 strain.

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Correspondence to Doolyi Kim.

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Kim, D., Tamai, Y., Azuma, T. et al. Analysis of the electrophoretic karyotype ofFlammulina velutipes . J Wood Sci 46, 466–469 (2000). https://doi.org/10.1007/BF00765805

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Key words

  • Flammulina velutipes
  • Electrophoretic karyotype
  • Chromosome
  • CHEF