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Journal of Wood Science

Official Journal of the Japan Wood Research Society

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Analysis of the electrophoretic karyotype ofFlammulina velutipes

Journal of Wood Science volume 46, pages 466–469 (2000)Cite this article

Abstract

The karyotype ofFlammulina velutipes (Curt.: Fr.) Sing. was analyzed electrophoretically using contourclamped homogeneous electric field gel electrophoresis and hybridization with DNA probes. The chromosomal DNA from the monokaryon (Fv-4K) and the dikaryon (Fv-4) were resolved into six and eight bands, respectively. The sizes of the chromosomes ranged from 1.9 to 6.0 megabase (Mb) pairs. Each of the separated bands of chromosomal DNA was identified by use of five cloned probes. The number of these chromosomes was estimated to be 6 and 12, respectively; and the size of the entire genome was estimated to be about 20.1 and 38.6Mb, respectively. From a comparison of the hybridization patterns, the existence of allelic chromosomes of different sizes was deduced in the Fv-4 strain.

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Authors and Affiliations

  1. Laboratory of Forest Pathology, Division of Forest Biology, Forestry Research Institute, 207 Chungryang 2-dong, 130-012, Dongdaemoon-gu, Seoul, Korea

    Doolyi Kim

  2. Division of Environmental Resources, Graduate School of Agriculture, Hokkaido University, N9-W9 Kita-ku, 060-8589, Sapporo, Japan

    Yutaka Tamai, Akira Ando & Kiyoshi Miura

  3. Forest Products Research Institute, 1-10 Nishi-kagura, 071-0181, Asahikawa, Japan

    Tomonori Azuma & Akira Harada

  4. Japan International Research Center for Agricultural Sciences, 1-2 Owashi, 305-8686, Tsukuba, Japan

    Yoh Sakuma

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  1. Doolyi Kim
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  2. Yutaka Tamai
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  3. Tomonori Azuma
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  4. Akira Harada
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  5. Akira Ando
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  6. Yoh Sakuma
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  7. Kiyoshi Miura
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Kim, D., Tamai, Y., Azuma, T. et al. Analysis of the electrophoretic karyotype ofFlammulina velutipes . J Wood Sci 46, 466–469 (2000). https://doi.org/10.1007/BF00765805

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  • DOI: https://doi.org/10.1007/BF00765805

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