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Analysis of the electrophoretic karyotype ofFlammulina velutipes

Abstract

The karyotype ofFlammulina velutipes (Curt.: Fr.) Sing. was analyzed electrophoretically using contourclamped homogeneous electric field gel electrophoresis and hybridization with DNA probes. The chromosomal DNA from the monokaryon (Fv-4K) and the dikaryon (Fv-4) were resolved into six and eight bands, respectively. The sizes of the chromosomes ranged from 1.9 to 6.0 megabase (Mb) pairs. Each of the separated bands of chromosomal DNA was identified by use of five cloned probes. The number of these chromosomes was estimated to be 6 and 12, respectively; and the size of the entire genome was estimated to be about 20.1 and 38.6Mb, respectively. From a comparison of the hybridization patterns, the existence of allelic chromosomes of different sizes was deduced in the Fv-4 strain.

References

  1. 1.

    Azuma T, Harada A, Kim DY, Sakuma Y, Kojima Y, Miura K (1996) Isolation of a gene specifically expressed during fruiting body differentiation inFlammulina velutipes. Mokuzai Gakkaishi 42:688–692

    CAS  Google Scholar 

  2. 2.

    Azuma T, Yao I, Kim DY, Sakuma Y, Kojima Y, Miura K (1996) Sequence and characterization of the genomic clone of the FDS gene from the basidiomyceteFlammulina velutipes. Mokuzai Gakkaishi 42:875–880

    CAS  Google Scholar 

  3. 3.

    Arima T, Morinaga T (1993) Electrophoretic karyotype ofLentinus edodes. Trans Mycol Soc Jpn 34:481–485

    CAS  Google Scholar 

  4. 4.

    Tamai Y, Yokono T, Sunagawa M, Kojima Y, Miura K (1995) Separation of large DNA molecules fromPleurotus cornucopiae by pulsed-field gel electrophoresis. Mokuzai Gakkaishi 41:952–955

    CAS  Google Scholar 

  5. 5.

    Sagawa I, Nagata Y (1992) Analysis of chromosomal DNA of mushrooms in genusPleurotus by pulsed-field gel electrophoresis. J Gen Appl Microbiol 38:47–52

    CAS  Article  Google Scholar 

  6. 6.

    Schwartz DC, Charles RC (1984) Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis. Cell 37:67–75

    CAS  Article  PubMed  Google Scholar 

  7. 7.

    Smith CL, Matsumoto T, Niwa O, Klco S, Fan JB, Yanagida M, Charles RC (1987) An electrophoretic karyotype forSchizosaccharomyces pombe by pulsed field gel electrophoresis. Nucleic Acids Res 15:4481–4489

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  8. 8.

    Doi M, Homma M, Chindamporn A, Tanaka K (1992) Estimation of chromosome number and size by pulsed-field gel electrophoresis (PFGE) in medically importantCandida species. J Gen Microbiol 138:2243–2251

    CAS  Article  PubMed  Google Scholar 

  9. 9.

    Iwaguchi S, Homma M, Tanaka K (1990) Variation in the electrophoretic karyotype analyzed by the assignment of DNA probes inCandida albicans. J Gen Microbiol 136:2433–2442

    CAS  Article  PubMed  Google Scholar 

  10. 10.

    Carter GL, Allison D, Rey MW, Dunn-Coleman NS (1992) Chromosomal and genetic analysis of the electrophoretic karyotype ofTrichoderma reesei: mapping of the cellulase and xylanase genes. Mol Microbiol 6:2167–2174

    CAS  Article  PubMed  Google Scholar 

  11. 11.

    Mantyla AL, Rossi KH, Vanhanen SA, Penttila ME, Suominen PL, Nevalainen KMH (1992) Electrophoretic karyotyping of wild-type and mutantTrichoderma longibrachiatum (reesei) strains. Curr Genet 21:471–477

    CAS  Article  PubMed  Google Scholar 

  12. 12.

    Brody H, John C (1989) Electrophoretic karyotype ofAspergillus nidulans. Proc Natl Acad Sci USA 6:6260–6263

    Article  Google Scholar 

  13. 13.

    Rustchenko-bulgac EP (1991) Variations ofCandida albicans electrophoretic karyotypes. J Bacteriol 173:6586–6596

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  14. 14.

    Orbach MJ, Vollrath D, Davis RW, Charles Y (1988) An electrophoretic karyotype ofNeurospora crassa. Mol Cell Biol 8:1469–1473

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  15. 15.

    Hayes CK, Harman GE, Woo SL, Gullino L, Lorito M (1993) Methods for electrophoretic karyotyping of filamentous fungi in the genusTrichoderma. Anal Biochem 209:176–182

    CAS  Article  PubMed  Google Scholar 

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Correspondence to Doolyi Kim.

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Kim, D., Tamai, Y., Azuma, T. et al. Analysis of the electrophoretic karyotype ofFlammulina velutipes . J Wood Sci 46, 466–469 (2000). https://doi.org/10.1007/BF00765805

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Key words

  • Flammulina velutipes
  • Electrophoretic karyotype
  • Chromosome
  • CHEF