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Karyotype analysis of interspecific fusants of basidiomycetes by pulsed-field gel electrophoresis

Abstract

The purpose of this research was to analyze the karyotype of the interspecific fusants of twoPleurotus species. Auxotrophic mutants derived from the cultivated strain ofP. ostreatus andP. cornucopiae were used. Protoplasts were fused electrically, and the fusants were selected under auxotrophic complementation. Esterase isozyme analysis showed that several fusants had isozyme bands originating from both parental strains, and others had unilateral isozyme bands. The fusant that had expressed isozyme bands of both parental strains showed chromosomal DNA bands of both of the parental strains in pulsedfield gel electrophoresis analysis. Despite the above results, the chromosomal composition of the fusants obtained by the pulsed-field gel electrophoresis did not exhibit all of the bands of both fusion parents.

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Correspondence to Takeshi Yokono.

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Yokono, T., Tamai, Y., Azuma, T. et al. Karyotype analysis of interspecific fusants of basidiomycetes by pulsed-field gel electrophoresis. J Wood Sci 46, 480–484 (2000). https://doi.org/10.1007/BF00765808

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Key words

  • Pleurotus species
  • Protoplast fusion
  • Electrophoretic karyotype
  • Chromosome
  • Mushroom