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Official Journal of the Japan Wood Research Society

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Karyotype analysis of interspecific fusants of basidiomycetes by pulsed-field gel electrophoresis

Journal of Wood Science volume 46, pages 480–484 (2000)Cite this article

Abstract

The purpose of this research was to analyze the karyotype of the interspecific fusants of twoPleurotus species. Auxotrophic mutants derived from the cultivated strain ofP. ostreatus andP. cornucopiae were used. Protoplasts were fused electrically, and the fusants were selected under auxotrophic complementation. Esterase isozyme analysis showed that several fusants had isozyme bands originating from both parental strains, and others had unilateral isozyme bands. The fusant that had expressed isozyme bands of both parental strains showed chromosomal DNA bands of both of the parental strains in pulsedfield gel electrophoresis analysis. Despite the above results, the chromosomal composition of the fusants obtained by the pulsed-field gel electrophoresis did not exhibit all of the bands of both fusion parents.

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Authors and Affiliations

  1. M&S Instruments Trading Inc., 113 Yarai-cho, Shinjuku-ku, 162-0805, Tokyo, Japan

    Takeshi Yokono

  2. Division of Environment and Resource, Graduate School of Agriculture, Hokkaido University, 060-0809, Sapporo, Japan

    Yutaka Tamai, Kiyoshi Miura & Yasuo Kojima

  3. Forest Products Research Institute, 071-0181, Asahikawa, Japan

    Tomonori Azuma

  4. Biological Resources Division, Japan International Research Center for Agricultural Sciences (JIRCAS), 305-8686, Ibaraki, Japan

    Yoh Sakuma

  5. Forestry and Forest Products Research Institute, 305-8687, Ibaraki, Japan

    Masahide Sunagawa

  6. Department of Biological Resource, Faculty of Agriculture, 399-4598, Nagano, Japan

    Masatake Ohmasa

Authors
  1. Takeshi Yokono
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  2. Yutaka Tamai
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  3. Tomonori Azuma
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  5. Kiyoshi Miura
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  7. Masahide Sunagawa
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  8. Masatake Ohmasa
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Correspondence to Takeshi Yokono.

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Yokono, T., Tamai, Y., Azuma, T. et al. Karyotype analysis of interspecific fusants of basidiomycetes by pulsed-field gel electrophoresis. J Wood Sci 46, 480–484 (2000). https://doi.org/10.1007/BF00765808

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  • DOI: https://doi.org/10.1007/BF00765808

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