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Journal of Wood Science

Official Journal of the Japan Wood Research Society

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Sinapyl alcohol-specific peroxidase isoenzyme catalyzes the formation of the dehydrogenative polymer from sinapyl alcohol

Journal of Wood Science volume 48, pages 497–504 (2002)Cite this article

Abstract

Two peroxidases, CWPO-A and CWPO-C, were isolated from the cell walls of poplar (Populus alba L.) callus culture. The cationic CWPO-C showed a strong preference for sinapyl alcohol over coniferyl alcohol as substrate. Thus, the monolignol utilization of CWPO-C is unique compared with other peroxidases, including anionic CWPO-A and horseradish peroxidase (HRP). CWPO-C polymerized oligomeric sinapyl alcohol (S-oligo) and sinapyl alcohol, producing a polymer of greater molecular weight. In contrast, HRP, which is specific to coniferyl alcohol, produced sinapyl alcohol dimers, rather than catalyzing polymerization. Adding coniferyl alcohol as a radical mediator in the HRP-mediated reaction did not result in S-oligo polymerization. This report shows that CWPO-C is an isoenzyme specific to sinapyl alcohol that polymerizes oligomeric lignols. Its catalytic activity toward oligomeric lignols may be related to the lignification of angiosperm woody plant cell walls.

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Author notes

  1. Yuji Tsutsumi

    Present address: Faculty of Agriculture, Kyushu University, Hakozaki, 812-8581, Fukuoka, Japan

Authors and Affiliations

  1. Department of Forest Resources Science, Shizuoka University, 422-8529, Shizuoka, Japan

    Wataru Aoyama, Shinya Sasaki, Shigeki Matsumura, Hirofumi Hirai, Yuji Tsutsumi & Tomoaki Nishida

  2. The United Graduate School of Agricultural Science, Gifu University, 501-1193, Gifu, Japan

    Wataru Aoyama

  3. Department of Bio Resources, Mie University, 514-8507, Tsu, Japan

    Thoru Mitsunaga

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  1. Wataru Aoyama
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Correspondence to Yuji Tsutsumi.

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Aoyama, W., Sasaki, S., Matsumura, S. et al. Sinapyl alcohol-specific peroxidase isoenzyme catalyzes the formation of the dehydrogenative polymer from sinapyl alcohol. J Wood Sci 48, 497–504 (2002). https://doi.org/10.1007/BF00766646

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  • DOI: https://doi.org/10.1007/BF00766646

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