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Journal of Wood Science

Official Journal of the Japan Wood Research Society

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Survey and enzymatic formation of lignans ofAnthriscus sylvestris

Journal of Wood Science volume 48, pages 536–541 (2002)Cite this article

Abstract

Gas chromatography — mass spectrometry analysis of the β-glucosidase-treated MeOH extracts ofAnthriscus sylvestris showed, based on comparison of the mass spectra and retention times with those of authentic samples, the presence of lignans, yatein, secoisolariciresinol, lariciresinol, matairesinol, hinokinin, and pluviatolide. The existence of small amounts of bursehernin was suggested by mass chromatography. In addition, nemerosin and deoxypodophyllotoxin were tentatively identified by comparing the mass spectra with those reported in the literature. Enzyme preparations fromA. sylvestris catalyzed the formation of secoisolariciresinol and lariciresinol from coniferyl alcohol. Furthermore, the enzyme preparation catalyzed the formation of lariciresinol from (±)-pinoresinols and the formation of secoisolariciresinol from (±)-lariciresinols. Thus, pinoresinol/lariciresinol reductase (PLR) activity was detected. Chiral high-performance liquid chromatography analysis showed selective formation of (+)-lariciresinol and (−)-secoisolariciresinol from (±)pinoresinols with theA. sylvestris PLR preparation, indicating that the stereochemical property ofA. sylvestris PLR-catalyzed reduction was similar to those ofForsythia PLR andArctium lappa ripening seed PLR.

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Authors and Affiliations

  1. Wood Research Institute, Kyoto University, Uji, 611-0011, Kyoto, Japan

    Shiro Suzuki, Norikazu Sakakibara, Toshiaki Umezawa & Mikio Shimada

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  1. Shiro Suzuki
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  2. Norikazu Sakakibara
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  3. Toshiaki Umezawa
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  4. Mikio Shimada
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Correspondence to Toshiaki Umezawa.

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Suzuki, S., Sakakibara, N., Umezawa, T. et al. Survey and enzymatic formation of lignans ofAnthriscus sylvestris . J Wood Sci 48, 536–541 (2002). https://doi.org/10.1007/BF00766653

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