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Official Journal of the Japan Wood Research Society

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Preparation of amphiphilic lignin derivative as a cellulase stabilizer

Abstract

A polymeric amphiphile, PE-AL, was prepared from acetic acid lignin (AL) obtained by acetic acid pulping of birch under atmospheric pressure with polyethylene glycol diglycidyl ether (PE) as the crosslinker. The behavior of PE-AL solutions and the complex formation of PE-AL with protein were investigated to clarify the function of this novel lignin derivative. The reduced viscosity of the amphiphile in aqueous solution was low (<0.3dl/g), and it decreased with increasing concentration in dilute solution. This suggested that the PE-AL in aqueous solution has a structure similar to that of Einstein's sphere and shrinks upon hydrophobic interaction among the structural moieties in AL and the exclusive volume effect. The amphiphilic PE-AL obviously formed a complex with bovine serum albumin (BSA) at 4°C with a reaction time of about 1 week. After complex formation with cellulase for 1 week, the cellulase activity of the resulting complex is significantly enhanced and is preserved after recycling the complex for hydrolysis of cellulosic materials several times.

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Correspondence to Yasumitsu Uraki.

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Uraki, Y., Ishikawa, N., Nishida, M. et al. Preparation of amphiphilic lignin derivative as a cellulase stabilizer. J Wood Sci 47, 301–307 (2001). https://doi.org/10.1007/BF00766717

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Key words

  • Acetic acid lignin
  • Amphiphile
  • Cellulase stabilizer
  • Complex formation with proteins
  • Polyethylene glycol diglycidyl ether
  • Viscosity