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Journal of Wood Science

Official Journal of the Japan Wood Research Society

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Preparation of amphiphilic lignin derivative as a cellulase stabilizer

Journal of Wood Science volume 47, pages 301–307 (2001)Cite this article

Abstract

A polymeric amphiphile, PE-AL, was prepared from acetic acid lignin (AL) obtained by acetic acid pulping of birch under atmospheric pressure with polyethylene glycol diglycidyl ether (PE) as the crosslinker. The behavior of PE-AL solutions and the complex formation of PE-AL with protein were investigated to clarify the function of this novel lignin derivative. The reduced viscosity of the amphiphile in aqueous solution was low (<0.3dl/g), and it decreased with increasing concentration in dilute solution. This suggested that the PE-AL in aqueous solution has a structure similar to that of Einstein's sphere and shrinks upon hydrophobic interaction among the structural moieties in AL and the exclusive volume effect. The amphiphilic PE-AL obviously formed a complex with bovine serum albumin (BSA) at 4°C with a reaction time of about 1 week. After complex formation with cellulase for 1 week, the cellulase activity of the resulting complex is significantly enhanced and is preserved after recycling the complex for hydrolysis of cellulosic materials several times.

References

  1. Smook GA (1992) Handbook for pulp and paper technologists, 2nd edn. Angus Wilde Publications, Vancouver, pp 74–83

    Google Scholar 

  2. Hoyt CH, Goheen DW (1971) Polymeric products. In: Sarkanen KV, Ludwig CH (eds) Lignins. Wiley Interscience, New York, pp 846–857

    Google Scholar 

  3. Sano Y, Nakamura M, Shimamoto S (1990) Pulping of wood at atmospheric pressure. II. Pulping of birch wood with aqueous acetic acid containing a small amount of sulfuric acid. Mokuzai Gakkaishi 36:207–211

    CAS  Google Scholar 

  4. Kubo S, Uraki Y, Sano Y (1996) Thermomechanical analysis of isolated lignins. Holzforschung 50:144–150

    Article  CAS  Google Scholar 

  5. Uraki Y, Kubo S, Nigo N, Sanoand Y, Sasaya T (1995) Preparation of carbon fibers from organosolv lignin obtained by aqueous acetic acid pulping. Holzforschung 49:343–350

    Article  CAS  Google Scholar 

  6. Uraki Y, Kubo S, Kurakami H, Sano Y (1997) Activated carbon fibers from acetic acid lignin. Holzforschung 51:188–192

    Article  CAS  Google Scholar 

  7. Kawamoto H, Nakatsubo H, Murakami K (1992) Protein-adsorbing capacities of lignin samples. Mokuzai Gakkaishi 38:81–84

    CAS  Google Scholar 

  8. Funaoka M (1998) Design and synthesis of functional lignin-based polymers. Cellulose Commun 5(1):13–18

    CAS  Google Scholar 

  9. Shimizu K, Sudo K, Nagasawa S, Ishihara M (1983) Enzymatic hydrolysis of woods. VII. Enzymatic susceptibility of autohydrolyzed woods. Mokuzai Gakkaishi 29:428–437

    CAS  Google Scholar 

  10. Kumakura M (1997) Preparation of immobilized cellulase beads and their application to hydrolysis of cellulosic materials. Process Biochem 31:555–559

    Article  Google Scholar 

  11. Woodward J (1989) Immobilized cellulase for cellulose utilization. J Biotechnol 11:299–312

    Article  CAS  Google Scholar 

  12. Karube I, Tanaka S, Shirai T, Suzuki S (1977) Hydrolysis of cellulose in a cellulase-bead fluidized bed reactor. Biotechnol Bioeng 19:1183–1191

    Article  CAS  Google Scholar 

  13. Rao M, Mishra C (1984) Properties and applications of penicillium funiculosum cellulase immobilized on a soluble polymer. Biotechnol Lett 6:319–322

    Article  CAS  Google Scholar 

  14. Hayashi T, Kinomura K, Sakakibara T, Okumura M, Tsuchiya A, Asada H (1996) Enzyme complex. Japan patent H08-89247

  15. Uraki Y, Sano Y, Sasaya T (1991) Cooking of hardwoods with organosolv pulping in aqueous acetic acid containing sulfuric acid at atmospheric pressure. Jpn TAPPI J 45:1018–1024

    Article  CAS  Google Scholar 

  16. Morgan PW (1946) Determination of ethers and esters of ethylene glycol: a modified alkoxyl analysis. Ind Eng Chem Anal Ed 18:500–504

    Article  CAS  Google Scholar 

  17. Kawamura I, Higuchi T (1964) Studies on the properties of lignins of plants in various taxonomical positions. II. On the I. R. absorption spectra of lignins. Mokuzai Gakkaishi 10:200–206

    CAS  Google Scholar 

  18. Kumakura M, Kaetsu I (1985) Fluid immobilized cellulase. Biotechnol Lett 7:773–778

    Article  CAS  Google Scholar 

  19. Tanaka M, Nakamura H, Taniguchi M, Morita T, Matsuo R, Kamikubo T (1986) Elucidation of adsorption processes of cellulases during hydrolysis if crystalline cellulose. Appl Microbiol Biotechnol 23:263

    Article  CAS  Google Scholar 

  20. Ooshima H, Sakata M, Harano Y (1986) Enhancement of enzymatic hydrolysis of cellulose by surfactant. Biotechnol Bioeng 28:1727–1734

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, 060-8589, Sapporo, Japan

    Yasumitsu Uraki, Naoya Ishikawa, Mitsukuni Nishida & Yoshihiro Sano

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  1. Yasumitsu Uraki
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  2. Naoya Ishikawa
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  3. Mitsukuni Nishida
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  4. Yoshihiro Sano
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Correspondence to Yasumitsu Uraki.

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Uraki, Y., Ishikawa, N., Nishida, M. et al. Preparation of amphiphilic lignin derivative as a cellulase stabilizer. J Wood Sci 47, 301–307 (2001). https://doi.org/10.1007/BF00766717

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  • DOI: https://doi.org/10.1007/BF00766717

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