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Journal of Wood Science

Official Journal of the Japan Wood Research Society

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Formation of callose from sucrose in cotton fiber microsomal membranes

Journal of Wood Science volume 47, pages 331–335 (2001)Cite this article

Abstract

Callose is formed from exogenous sucrose by cotton fiber microsomal membranes that contain both sucrose synthase and callose synthase activity. Although the coupled reaction between sucrose and callose synthases occurs predominantly to channel glucose from sucrosederived uridine diphosphate (UDP)-glucose into callose in the membranes, there is no difference in the UDP-glucose-forming/sucrose-forming activity ratios between the soluble and membrane-bound forms of sucrose synthase. The consumption of UDP-glucose from sucrose into callose probably leads to UDP-glucose formation rather than sucrose formation despite the lower affinity of sucrose synthase for sucrose than for UDP-glucose. Callose formation is markedly stimulated by the addition of either recombinant Glu11 (S11E) or in vitro phosphorylated Ser11 mung bean sucrose synthase but not by the wild-type nonphosphorylated Ser11 enzyme. We propose that a negative charge (by phosphorylation or mutagenesis) at Ser11 in sucrose synthase causes the enzyme to promote a coupled callose-forming reaction.

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Author notes

  1. Tomonori Nakai

    Present address: Research Laboratory of Resources Utilization, Tokyo Institute of Technology, 226-8503, Yokohama, Japan

Authors and Affiliations

  1. Wood Research Institute, Kyoto University, Uji, 611-0011, Kyoto, Japan

    Teruko Konishi, Tomonori Nakai, Fukumi Sakai & Takahisa Hayashi

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  1. Teruko Konishi
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  2. Tomonori Nakai
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  3. Fukumi Sakai
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  4. Takahisa Hayashi
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Correspondence to Takahisa Hayashi.

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Konishi, T., Nakai, T., Sakai, F. et al. Formation of callose from sucrose in cotton fiber microsomal membranes. J Wood Sci 47, 331–335 (2001). https://doi.org/10.1007/BF00766781

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