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Determination of nitrobenzene oxidation products by GC and1H-NMR spectroscopy using 5-iodovanillin as a new internal standard

Abstract

The nitrobenzene oxidation method was modified to obtain more reproducible data and more structural information about lignin, not only by gas chromatography (GC) but also by proton nuclear magnetic resonance (1H-NMR) spectroscopy for quantitative determination of the oxidation products and to simplify the procedures. The nitrobenzene oxidation mixture was directly extracted after acidification without preextraction of by-products. The direct extraction made the extractive step easy and gave reproducible data. 5-Iodovanillin was selected as a new internal standard. The reason for this selection was that 5-iodovanillin did not exist in the nitrobenzene oxidation products from any plant species and had an aldehyde group whose peak did not overlap with the other aldehyde peaks on an1H-NMR spectrum. Thus, the use of 5-iodovanillin enabled us to quantifyp-hydroxybenzaldehyde, vanillin, and syringaldehyde in oxidation products on the basis of1H-NMR analysis as well as GC. Furthermore, more information about the condensed structure of lignin was derived by comparing the1H-NMR and GC analyses.

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Correspondence to Rui Katahira.

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Katahira, R., Nakatsubo, F. Determination of nitrobenzene oxidation products by GC and1H-NMR spectroscopy using 5-iodovanillin as a new internal standard. J Wood Sci 47, 378–382 (2001). https://doi.org/10.1007/BF00766789

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Key words

  • Lignin
  • 5-Iodovanillin
  • Modified nitrobenzene oxidation
  • 1H-NMR analysis