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Official Journal of the Japan Wood Research Society

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Transferase activity of GhCesA2 (putative cotton cellulose 4-β-glucosyltransferase) expressed inPichia pastoris

Abstract

GhCesA2 is a cotton (Gossypium hirsutum) homologue of the bacterial cellulose synthase gene that encodes cellulose 4-β-glucosyltransferase. The central catalytic region of GhCesA2 was expressed as a soluble protein in the methylotrophic yeastPichia pastoris. The molecular size of the recombinant protein was 100kDa, which decreased to 85 kDa after treatment with endoglycosidase H. The recombinant GhCesA2 catalyzed transfer of glucose from UDP-glucose into unknown products in the presence of an extract of cotton hypocotyls, but the products were notβ-1,4-glucan.

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Correspondence to Takahisa Hayashi.

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Ihara, Y., Takeda, T., Sakai, F. et al. Transferase activity of GhCesA2 (putative cotton cellulose 4-β-glucosyltransferase) expressed inPichia pastoris . J Wood Sci 48, 425–428 (2002). https://doi.org/10.1007/BF00770704

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