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Journal of Wood Science

Official Journal of the Japan Wood Research Society

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Coniferyl aldehyde dimers in dehydrogenative polymerization: model of abnormal lignin formation in cinnamyl alcohol dehydrogenase-deficient plants

Journal of Wood Science volume 48, pages 216–221 (2002)Cite this article

Abstract

The enzymatically dehydrogenative polymerization of coniferyl aldehyde and coniferyl alcohol was studied to understand lignins in cinnamyl alcohol dehydrogenase (CAD)-downregulated plants. The sample dimers were prepared by polymerization under three reaction systems (coniferyl alcohol, coniferyl aldehyde, and their combination) with horseradish peroxidase/H2O2 under the conditions of limited reaction time. In addition, the residual amount of substrate in each reaction was determined at specified time intervals. In the reaction system of coniferyl aldehyde, the 5-5-type dimer was formed in preference toβ-β andβ-5 dimers; in the reaction system of coniferyl alcohol theβ-5 dimer was preferentially formed. Furthermore, it was revealed when quantifying dimers among reaction systems that the total dimer formation capability of coniferyl alcohol clearly surpassed that of coniferyl aldehyde. However, the dimers cross-coupled with coniferyl alcohol and coniferyl aldehyde were formed in amounts not accounted for by the difference seen in dimer formation abilities with the two substrates.

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Authors and Affiliations

  1. United Graduate School of Agricultural Science, Gifu University, 501-1193, Gifu, Japan

    Takashi Ito

  2. Department of Applied Bioorganic Chemistry, Faculty of Agriculture, Gifu University, 1-1 Yanagido, 501-1193, Gifu, Japan

    Reiko Hayase, Shingo Kawai & Hideo Ohashi

  3. Kyoto University, 611-0011, Kyoto, Japan

    Takayoshi Higuchi

Authors
  1. Takashi Ito
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  2. Reiko Hayase
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  3. Shingo Kawai
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  4. Hideo Ohashi
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  5. Takayoshi Higuchi
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Correspondence to Hideo Ohashi.

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Ito, T., Hayase, R., Kawai, S. et al. Coniferyl aldehyde dimers in dehydrogenative polymerization: model of abnormal lignin formation in cinnamyl alcohol dehydrogenase-deficient plants. J Wood Sci 48, 216–221 (2002). https://doi.org/10.1007/BF00771370

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  • DOI: https://doi.org/10.1007/BF00771370

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