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Official Journal of the Japan Wood Research Society

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Microfibril angle of Norway spruce [Picea abies (L.) Karst.] compression wood: comparison of measuring techniques


The structure of cellulose, especially the microfibril angles (MFAs), in compression wood of Norway spruce [Picea abies (L.) Karst.] was studied by wide- and small-angle X-ray scattering and polarizing microscopy. On the basis of the X-ray scattering experiments the average MF As of the cell wall layers S2 and S1 of the studied sample are 39‡ and 89‡, respectively; and the average diameter and length of the cellulose crystallites are 2.9 and 20.0nm, respectively. The average of the whole MFA distribution is shown to agree with the one obtained by polarizing microscopy of macerated fibers.


  1. 1.

    Harris JM, Meylan BA (1965) The influence of microfibril angle on longitudinal and tangential shrinkage inPinus radiata. Holzforschung 19(5):144–153

    Article  Google Scholar 

  2. 2.

    Cave ID (1968) The anisotropic elasticity of the plant cell wall. Wood Sci Technol 2:268–278

    Article  Google Scholar 

  3. 3.

    Leney L (1981) A technique for measuring fibril angle using polarized light. Wood Fiber 13(1):13–16

    Google Scholar 

  4. 4.

    Donaldson LA (1991) The use of pit apertures as windows to measure microfibril angle in chemical pulp fibers. Wood Fiber Sci 23:290–295

    CAS  Google Scholar 

  5. 5.

    DeLuca LB, Orr RS (1961) Crystallite orientation and spiral structure of cotton. J Polym Sci 54:457–470

    CAS  Article  Google Scholar 

  6. 6.

    Cave ID (1966) Theory of X-ray measurement of microfibril angle in wood. For Prod J 16(10):37–42

    Google Scholar 

  7. 7.

    Boyd JD (1977) Interpretation of X-ray diffractograms of wood for assessments of microfibril angles in fibre cell walls. Wood Sci Technol 11:93–114

    Article  Google Scholar 

  8. 8.

    Sobue N, Hirai N, Asano I (1971) Studies on structure of wood by X-ray. II. Estimation of the orientation of micells in cell wall. J Jpn Wood Res Soc 17(2):44–50

    Google Scholar 

  9. 9.

    Paakkari T, Serimaa R (1984) A study of the structure of wood cells by X-ray diffraction. Wood Sci Technol 18:79–85

    Google Scholar 

  10. 10.

    Cave ID (1997) Theory of X-ray measurement of microfibril angle in wood. Wood Sci Technol 31:225–234

    CAS  Article  Google Scholar 

  11. 11.

    Wardrop AB (1952) The low-angle scattering of X-rays by conifer tracheids. Textile Res J 22:288–291

    CAS  Article  Google Scholar 

  12. 12.

    Kantola M, Seitonen S (1961) X-ray orientation investigations. Ann Acad Sci Fenn A VI Phys 80:1–15

    Google Scholar 

  13. 13.

    Kantola M, Kähkönen H (1963) Small-angle X-ray investigation. Ann Acad Sci Fenn A VI Phys 137:1–14

    Google Scholar 

  14. 14.

    Kantola M, Kähkönen H, Seitonen S (1965) On the correspondence of the small-angle and wide-angle X-ray diffraction patterns of wood fibers. Ann Acad Sci Fenn A VI Phys 220:1–9

    Google Scholar 

  15. 15.

    Jakob HF, Fratzl P, Tschegg SE (1994) Size and arrangement of elementary cellulose fibrils in wood cells: a small-angle X-ray scattering study ofPicea abies. J Struct Biol 113:13–22

    Article  Google Scholar 

  16. 16.

    Lichtenegger H, Reiterer A, Tschegg S, Fratzl P (1998) Determination of spiral angles of elementary fibrils in the wood cell wall: comparison of small-angle X-ray scattering and wide-angle X-ray diffraction. In: Butterfield BG (ed) Microfibril angle in wood. University of Canterbury, Christchurch, pp 140–156

    Google Scholar 

  17. 17.

    Reiterer A, Jakob HF, Stanzl-Tschegg SE, Fratzl P (1998) Spiral angle of elementary cellulose fibrils in cell walls ofPicea abies determined by small-angle X-ray scattering. Wood Sci Technol 32:335–345

    CAS  Article  Google Scholar 

  18. 18.

    Jakob HF, Fengel D, Tschegg SE, Fratzl P (1995) The elementary cellulose fibril inPicea abies: comparison of transmission electron microscopy, small-angle X-ray scattering, and wide-angle X-ray scattering results. Macromolecules 28:8782–8787

    CAS  Article  Google Scholar 

  19. 19.

    Heyn ANJ (1955) Small particle X-ray scattering by fibers; size and shape of microcrystallites. J Appl Phys 26:519–526

    CAS  Article  Google Scholar 

  20. 20.

    Timell TE (1986) Compression wood in gymnosperms (vol 1). Springer, Berlin, pp 157–167, 195–198

    Google Scholar 

  21. 21.

    Sugiyama J, Vuong R, Chanzy H (1991) Electron diffraction study on the two crystalline phases occurring in native cellulose from algal cell wall. Macromolecules 24:4168–4175

    CAS  Article  Google Scholar 

  22. 22.

    Franklin GL (1945) Preparation of thin sections of synthetic resins and wood resin composites and a new macerating method for wood. Nature 155:51

    Article  Google Scholar 

  23. 23.

    Jakob HF, Tschegg SE, Fratzl P (1996) Hydration dependence of the wood-cell wall structure inPicea abies: a small-angle X-ray scattering study. Macromolecules 29:8435–8440

    CAS  Article  Google Scholar 

  24. 24.

    Schmidt PW (1991) Small-angle scattering studies of disordered, porous and fractal systems. J Appl Cryst 24:414–435

    CAS  Article  Google Scholar 

  25. 25.

    Müller M, Czihak C, Vogl G, Fratzl P, Schober H, Riekel C (1992) Direct observation of microfibril arrangement in a single native cellulose fiber by microbeam small-angle X-ray scattering. Macromolecules 31:3953–3957

    Article  Google Scholar 

  26. 26.

    Prud'homme RE, Noah J (1975) Determination of fibril angle distribution in wood fibers: a comparison between the X-ray diffraction and the polarized microscope methods. Wood Fibers 6:282–289

    Google Scholar 

  27. 27.

    Huang C-L, Kutscha NP, Leaf GJ, Megraw RA (1998) Comparation of microfibril angle measurement techniques. In: Butterfield BG (ed) Microfibril angle in wood. University of Canterbury, Christchurch, pp 177–205

    Google Scholar 

  28. 28.

    Saranpää P, Serimaa R, Andersson S, Pesonen E, Suni T, Paakkari T (1998) Variation of microfibril angle of Norway spruce (Pinus abies (L.) Karst.) and Scots pine (Pinus sylvestris L.) — comparing X-ray diffraction and optical methods. In: Butterfield BG (ed) Microfibril angle in wood. University of Canterbury, Christchurch, pp 240–252

    Google Scholar 

  29. 29.

    Sahlberg U, Salmén L, Oscarsson A (1997) The fibrillar orientation in the S2-layer of wood fibres as determined by X-ray diffraction analysis. Wood Sci Technol 31:77–86

    CAS  Article  Google Scholar 

  30. 30.

    Saranpää P, Pesonen E, Sarén M, Andersson S, Siiriä S, Serimaa R, Paakkari T (in press) Variation on the properties of tracheids in Norway spruce (Picea abies (L.) Karst). In: Savidge R, Barnett J, Napier R (eds) Cambium: the biology of wood formation. BIOS Scientific, Oxford

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Correspondence to Ritva Serimaa.

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Andersson, S., Serimaa, R., Torkkeli, M. et al. Microfibril angle of Norway spruce [Picea abies (L.) Karst.] compression wood: comparison of measuring techniques. J Wood Sci 46, 343–349 (2000).

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Key words

  • Microfibril angle
  • Wide angle X-ray scattering
  • Small-angle X-ray scattering
  • Wood cellulose
  • Picea abies (L.) Karst