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Screening of wood-rotting fungi for kraft pulp bleaching by the Poly R decolorization test and biobleaching of hardwood kraft pulp byPhanerochaete crassa WD1694


From among 419 wood-rotting fungi 10 were selected by the Poly R decolorization test, and their ability to bleach hardwood kraft pulp was assayed. Of the 10 selected, 6 fungi (i.e.,Phanerochaete crassa WD1694 and F150;Pleurotus pulmonarius PSC-H, PSC-M, and PSC-T;and Pleurotus species A119) showed much higher bleaching ability thanPhanerochaete chrysosponum BKMF1767 orTrametes versicolor WD1670, both of which are well-known high ligninolytic fungi.P. crassa WD1694 had the highest bleaching ability among the selected strains, and it increased the pulp brightness from 28 to 54, with a corresponding decrease in kappa number from 16 to 6 after 10 days of cultivation and alkali extraction. MnP was a predominant ligninolytic enzyme ofP. crassa WD1694 during the biobleaching.


  1. 1.

    Raid ID, Paice MG (1994) Biological bleaching of kraft pulps by white-rot fungi and their enzymes. FEMS Microbiol Rev 13:369–376

    Article  Google Scholar 

  2. 2.

    Kirk TK, Yang HH (1979) Partial deligninfication of unbleached kraft pulp with ligninolytic fungi. Biotechnol Lett 1:347–352

    CAS  Article  Google Scholar 

  3. 3.

    Paice MG, Reid ID, Bourbonnais R, Archibald FS, Jurasek L (1993) Manganese peroxidase, produced byTrametes versicolor during pulp bleaching, demethylates and delignifies kraft pulp. Appl Environ Microbiol 59:260–265

    CAS  PubMed  PubMed Central  Google Scholar 

  4. 4.

    Kondo R, Harazono K, Sakai K (1994) Bleaching of hardwood kraft pulp with manganese peroxidase secreted fromPhanerochaete sordida YK-624. Appl Environ Microbiol 60:4359–4363

    CAS  PubMed  PubMed Central  Google Scholar 

  5. 5.

    Katagiri N, Tsutsumi Y, Nishida T (1995) Correlation of brightning with cumulative enzyme activity related to lignin biodegradation during biobleaching of kraft pulp by white rot fungi in the solidstate fermentation system. Appl Environ Microbiol 61:617–622

    CAS  PubMed  PubMed Central  Google Scholar 

  6. 6.

    Hirai H, Kondo R, Sakai K (1994) Screening of lignindegrading fungi and their ligninolytic enzyme activities during biological bleaching of kraft pulp. Mokuzai Gakkaishi 40:980–986

    CAS  Google Scholar 

  7. 7.

    Nishida T, Kashino Y, Mimura A, Takahara Y (1988) Lignin biodegradation by wood-rotting fungi. I. Screening of lignindegrading fungi. Mokuzai Gakkaishi 34:530–536

    Google Scholar 

  8. 8.

    Fujita K, Kondo R, Sakai K, Kashino Y, Nishida T, Takahara Y (1991) Biobleaching of kraft pulp using white-rot fungus IZU-154. TAPPI 74:123–127

    CAS  Google Scholar 

  9. 9.

    Paszczynski A, Pasti MB. Goszczynski S, Crawford DL, Crawford RL (1991) New approach to improve degradation of recalcitrant azo dyes byStreptomyces spp. andPhanerochaete chrysosporium. Enzyme Microbiol Technol 13:378–384

    CAS  Article  Google Scholar 

  10. 10.

    Cripps C, Bumpus JA, Aust SD (1990) Biodegradation of azo and heterocyclic dyes byPhanerochaete chrysosporium. Appl Environ Microbiol 56:1114–1118

    CAS  PubMed  PubMed Central  Google Scholar 

  11. 11.

    Kim SJ, Ishikawa K, Hirai M, Shoda M (1995) Characteristics of a newly isolated fungus,Geotrichum candidum Dec 1, which decolorizes various dyes. J Ferment Bioeng 79:601–607

    CAS  Article  Google Scholar 

  12. 12.

    Thorn RG (1993) The use of cellulose azure agar as a crude assay of both cellulolytic and ligninolytic abilities of wood-inhabiting fungi. Proc Jpn Acad 69:29–34

    CAS  Article  Google Scholar 

  13. 13.

    Glenn JK, Gold MH (1983) Decolorization of several polymeric dyes by the lignin-degrading basidiomycetePhanerochaete chrysosporium. Appl Environ Microbiol 45:1741–1747

    CAS  PubMed  PubMed Central  Google Scholar 

  14. 14.

    Gold MH, Glenn JK, Alic M (1988) Use of polymeric dyes in lignin biodegradation assays. Methods Enzymol 161:74–78

    CAS  Article  Google Scholar 

  15. 15.

    Iimori T, Kaneko R, Yoshihara H, Machida M, Yoshioka H, Murakami K (1994) Screening of pulp-bleaching fungi and bleaching activity of newly isolated fungus SKB-1152. Mokuzai Gakkaishi 40:733–737

    CAS  Google Scholar 

  16. 16.

    Kaneko R, Iimori T, Miyawaki S, Machida M, Murakami K (1995) Biobleaching with manganese peroxidase purified from the pulp bleaching fungus SKB-1152. Biosci Biotechnol Biochem 59:1584–1585

    CAS  Article  Google Scholar 

  17. 17.

    Buckley KF, Dobson ADW (1998) Extracellular ligninolytic enzyme production and polymeric dye decolorization in immobilized cultures ofChrysosporium lignorum CL1. Biotechnol Lett 20:301–306

    CAS  Article  Google Scholar 

  18. 18.

    Baldrian P, Gabriel J, Nerud F (1996) Effect of cadmium on the ligninolytic activity ofStereum hirstum andPhanerochaete chrysosporium. Folia Microbiol 41:363–367

    CAS  Article  Google Scholar 

  19. 19.

    Addleman K, Dumonceaux T, Paice MG, Bourbonnais R, Archibald FS (1995) Production and characterization ofTrametes versicolor mutants unable to bleach hardwood kraft pulp. Appl Environ Microbiol 61:3687–3694

    CAS  PubMed  PubMed Central  Google Scholar 

  20. 20.

    Glenn JK, Gold MH (1985) Purification and characterization of an extracellular manganese(II)-dependent peroxidase from the lignin-degrading basidiomycetePhanerochate chrysosporium. Arch Biochem Biophys 242:329–341

    CAS  Article  Google Scholar 

  21. 21.

    Commanday F, Macy JM (1985) Effect of substrate nitrogen on lignin degradation byPleurotus ostreatus. Arch Microbiol 142:61–65

    CAS  Article  Google Scholar 

  22. 22.

    Kuwahara M, Glenn JK, Morgan MA, Gold MH (1984) Separation and characterization of the extracellular H2O2-dependent oxidases from ligninolytic cultures ofPhanerochaete chrysosporium. FEBS Lett 169:247–250

    CAS  Article  Google Scholar 

  23. 23.

    Ollikka P, Alhonmaki K, Leppanen VM, Glumoff T, Raijiola T, Suominen I (1993) Decolorization of azo, triphenyl methane, heterocyclic, and polymeric dyes by lignin peroxidase isoenzymes fromPhanerochaete chrysosporium. Appl Environ Microbiol 59:4010–4016

    CAS  PubMed  PubMed Central  Google Scholar 

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Correspondence to Mariko Takano.

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Takano, M., Nishida, A. & Nakamura, M. Screening of wood-rotting fungi for kraft pulp bleaching by the Poly R decolorization test and biobleaching of hardwood kraft pulp byPhanerochaete crassa WD1694. J Wood Sci 47, 63–68 (2001).

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Key words

  • Fungi
  • Screening
  • Poly R
  • Lignin
  • Kraft pulp
  • Bleaching