Skip to main content

Official Journal of the Japan Wood Research Society

Journal of Wood Science Cover Image

Polyethylene degradation by manganese peroxidase in the absence of hydrogen peroxide

Abstract

A possible role of Tween 80 in the polyethylene degradation by manganese peroxidase (MnP) and the basis of the MnP action in the absence of hydrogen peroxide were investigated. The MnP activity in the system was retained its maximum level for 6 days in the presence of Tween 80. Tween 20 and CHAPSO stabilized MnP in the system similarly to Tween 80, and these surfactants also promote the polyethylene degradation. The system containing malonate buffer, Mn(II), and MnP produced Mn(III) in the absence of hydrogen peroxide, but the effect of Tween 80 addition on Mn(III) production in the absence of hydrogen peroxide was small. The results show that Mn(III) is generated by the MnP action initiated and amplified by the decomposition of malonate by Mn(III) and that a surfactant such as Tween 80 is required to stabilize MnP in the system.

References

  1. 1.

    Iiyoshi Y, Tsutsumi Y, Nishida T (1998) Polyethylene degradation by lignin-degrading fungi and manganese peroxidase. J Wood Sci 44:222–229

    CAS  Article  Google Scholar 

  2. 2.

    Bao W, Fukushima Y, Jensen KAJ, Moen MA, Hammel KE (1994) Oxidative degradation of non-phenolic lignin during lipid peroxidation by fungal manganese peroxidase. FEBS Lett 354:297–300

    CAS  Article  PubMed  Google Scholar 

  3. 3.

    Moen MA, Hammel KE (1994) Lipid peroxidation by the manganese peroxides ofPhanerochaete chrysosporium is the basis for phenanthrene oxidation by the intact fungus. Appl Environ Microbiol 60:1956–1961

    CAS  PubMed  PubMed Central  Google Scholar 

  4. 4.

    Hofrichter M, Ziegenhagen D, Vares T, Friedrich M, Jager MG, Fritsche W, Hatakka A (1998) Oxidative decomposition of malonic acid as basis for the action of manganese peroxidase in the absence of hydrogen peroxide. FEBS Lett 434:362–366

    CAS  Article  PubMed  Google Scholar 

  5. 5.

    Wariishi H, Valli K, Gold MH (1992) Manganese (II) oxidation by manganese peroxidase from basidiomycetePhanerochaete chrysosporium, kinetic mechanism and role of chelators. J Biol Chem 267:23688–23695

    CAS  PubMed  Google Scholar 

  6. 6.

    Archibald FS, Fridovich I (1982) The scavenging of Superoxide radical by manganous complexes: in vitro. Arch Biochem Biophys 214:452–463

    CAS  Article  PubMed  Google Scholar 

  7. 7.

    Ehara K, Tsutsumi Y, Nishida T (1997) Biobleaching of softwood and hardwood kraft pulp with manganese peroxidase. Mokuzai Gakkaishi 43:861–868

    CAS  Google Scholar 

  8. 8.

    Ehara K, Tsutsumi Y, Nishida T (1998) Structural changes of residual lignin in softwood kraft pulp treated with manganese peroxidase. J Wood Sci 44:327–331

    CAS  Article  Google Scholar 

  9. 9.

    Kondo R, Harazono K, Sakai K (1994) Bleaching of hardwood kraft pulp with manganese peroxidase secreted fromPhanerochaete sordida YK-624. Appl Environ Microbiol 60:4359–4363

    CAS  PubMed  PubMed Central  Google Scholar 

  10. 10.

    Harazono K, Kondo R, Sakai K (1996) Bleaching of hardwood kraft pulp with manganese peroxidase secreted fromPhanerochaete sordida YK-624 without addition of MnSO4. Appl Environ Microbiol 62:913–917

    CAS  PubMed  PubMed Central  Google Scholar 

  11. 11.

    Venkatadri R, Irvine R (1990) Effect of agitation ligninase activity and ligninase production byPhanerochaete chrysosporium Appl Environ Microbiol 56:2684–2691

    CAS  PubMed  PubMed Central  Google Scholar 

  12. 12.

    Iiyoshi Y, Tsutsumi Y, Nishida T (1997) Polyethylene degradation of white rot fungi (III). In: Proceedings of the 42nd lignin symposium, Sapporo, pp 189–190

  13. 13.

    Iiyoshi Y, Tsutsumi Y, Nishida T (1998) Polyethylene degradation of white rot fungi (IV). In: Proceedings of the 43rd lignin symposium, Tokyo, pp 97–100

  14. 14.

    Hastings D, Emerson S (1986) Oxidation of manganese by spores of a marine bacillus: kinetic and thermodynamic considerations. Geochim Cosmochim Acta 50:1819–1824

    CAS  Article  Google Scholar 

  15. 15.

    Urzua U, Kersten PJ, Vicuna R (1998) Manganese peroxidasedependent oxidation of glyoxylic and oxalic acids synthesized byCeriporiopsis subvermispora produces extracellular hydrogen peroxide. Appl Environ Microbiol 64:68–73

    CAS  PubMed  PubMed Central  Google Scholar 

Download references

Author information

Affiliations

Authors

Corresponding author

Correspondence to Yuji Tsutsumi.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Ehara, K., Iiyoshi, Y., Tsutsumi, Y. et al. Polyethylene degradation by manganese peroxidase in the absence of hydrogen peroxide. J Wood Sci 46, 180–183 (2000). https://doi.org/10.1007/BF00777369

Download citation

Key words

  • Polyethylene
  • Biodegradation
  • Manganese peroxidase
  • Surfactant