Skip to main content

Official Journal of the Japan Wood Research Society

Journal of Wood Science Cover Image

Wood identification of JapaneseCyclobalanopsis species (Fagaceae) based on DNA polymorphism of the intergenic spacer betweentrnT andtrnL 5′ exon

Abstract

DNA was extracted from wood samples of six representativeCyclobalanopsis species (Fagaceae) growing in Japan that cannot be distinguished from one another by conventional microscopy. A part of the intergenic spacer region betweentrnT andtrnT 5′ exon was amplified and sequenced. The sequences obtained from wood samples were grouped into three DNA types by a single nucleotide polymorphism as reported previously in leaf samples: I (Quercus acuta, Q. sessilifolia, Q. salicina), II (Q. myrsinaefolia, Q. glauca), and III (Q. gilva). Thus,Q. gilva can be distinguished from the otherQuercus species, and the others are separated in two subgroups based on DNA polymorphism. The present findings support the possibility of wood identification based on DNA polymorphism.

References

  1. 1.

    Wheeler EA, Baas P, Gasson PE (eds) (1989) IAWA list of microscopic features for hardwood identification. IAWA Bull 10:219–332

    Article  Google Scholar 

  2. 2.

    Wheeler EA, Baas P (1998) Wood identification: a review. IAWA Bull 19:241–264

    Article  Google Scholar 

  3. 3.

    Sudo S (1959) Identification of Japanese hardwoods (in Japanese). Bull Govt For Exp Station 118:1–138

    Google Scholar 

  4. 4.

    Saiki RK, Scharf S, Faloona F, Mullis KB, Horn GT, Erlich HA, Arnheim N (1985) Enzymatic amplification ofβ-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science 230:1350–1354

    CAS  Article  PubMed  Google Scholar 

  5. 5.

    Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, Horn GT, Mullis KB, Erlich HA (1988) Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239:487–491

    CAS  Article  PubMed  Google Scholar 

  6. 6.

    Shiraishi S, Watanabe A (1995) Identification of chloroplast genome betweenPinus densiflora SIEB, et ZUCC. andP. thunbergii PARL based on the polymorphism inrbcL gene (in Japanese). J Jpn For Soc 77:429–436

    Google Scholar 

  7. 7.

    Shiraishi S, Isoda K, Watanabe A, Kawasaki H (1996) DNA systematical study on theLarix relicted at Mt. Manokami, the Zao Mountains (in Japanese). J Jpn For Soc 78:175–182

    Google Scholar 

  8. 8.

    Ohyama M, Baba K, Itoh T, Shiraishi S (1999) Polymorphism analysis of Fagaceae and DNA-based identification ofFagus species grown in Japan based onrbcL gene. J Wood Sci 45:183–187

    CAS  Article  Google Scholar 

  9. 9.

    Ohyama M, Baba K, Itoh T (1999) Possibility of grouping ofCyclobalanopsis species (Fagaceae) grown in Japan based on an analysis of several regions of chloroplast DNA. J Wood Sci 45:498–501

    CAS  Article  Google Scholar 

  10. 10.

    Kitamura S, Murata G (1979) Coloured illustrations of woody plant of Japan (in Japanese), vol II. Hoikusha, Osaka

  11. 11.

    Shimaji K (1962) Anatomical studies on the phylogenetic interrelationship of the genera in the Fagaceae. Bull Tokyo Univ For 57:1–60

    Google Scholar 

  12. 12.

    Comes HP, Kadereit JW (1998) The effect of quaternary climatic changes on plant distribution and evolution. Trends Plant Sci 3:432–438

    Article  Google Scholar 

  13. 13.

    Murray MG, Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8:4321–4325

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  14. 14.

    Edward MG, Ann B, Paul W (1996) Effect on highly fragmented DNA on PCR. Nucleic Acids Res 24:5026–5033

    Article  Google Scholar 

  15. 15.

    Germano J, Klein AS (1999) Species-specific nuclear and chloroplast single nucleotide polymorphisms to distinguishPicea glauca. P. mariana andP. rubens. Theor Appl Genet 99:37–49

    CAS  Article  Google Scholar 

  16. 16.

    Suyama Y, Kawamuro K, Kinoshita I, Yoshimura K, Tsumura Y, Takahara H (1996) DNA sequence from a fossil pollen ofAbies spp. from Pleistocene peat. Genes Genet Syst 71:145–149

    CAS  Article  PubMed  Google Scholar 

  17. 17.

    Clegg MT, Gaut BS, Eearn GH, Morton BR (1994) Rates and patterns of chloroplast DNA evolution. Proc Natl Acad Sci USA 91:6795–6801

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  18. 18.

    Dumolin-Eapégue S, Pemonge MH, Gielly L, Taberlet P, Petit RJ (1999) Amplification of oak DNA from ancient and modern wood. Mol Ecol 8:2137–2140

    Article  Google Scholar 

Download references

Author information

Affiliations

Authors

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Ohyama, M., Baba, K. & Itoh, T. Wood identification of JapaneseCyclobalanopsis species (Fagaceae) based on DNA polymorphism of the intergenic spacer betweentrnT andtrnL 5′ exon. J Wood Sci 47, 81–86 (2001). https://doi.org/10.1007/BF00780554

Download citation

Key words

  • Wood identification
  • Cyclobalanopsis
  • Fagaceae
  • Chloroplast DNA