Skip to main content

Official Journal of the Japan Wood Research Society

Biobleaching of unbleached and oxygen-bleached hardwood kraft pulp by culture filtrate containing manganese peroxidase and lignin peroxidase fromPhanerochaete chrysosporium

Abstract

Unbleached and oxygen-bleached hardwood kraft pulp (UKP and OKP), respectively, were bleached with a culture filtrate containing manganese peroxidase (MnP) and lignin peroxidase (LiP) fromPhanerochaete chrysosporium Burds. The brightness increases of UKP upon biobleaching with the culture filtrate with and without MnSO4 were the same. The brightness increase of OKP with MnSO4 decreased to about half that seen without MnSO4. Changes in the brightness of UKP and OKP by treatment with the culture filtrate were determined. The brightness increased sharply by about eight points during the first 3h. The 3-h treatment was repeated seven times. The brightness increased linearly with the bleaching of UKP. On bleaching of OKP, the brightness increased slowly and stopped at about 78%.

References

  1. 1.

    Kirk TK, Yang HH (1979) Partial deligninfication of unbleached kraft pulp with ligninolytic fungi. Biotechnol Lett 1:347–352

    CAS  Article  Google Scholar 

  2. 2.

    Tran AV, Chambers RP (1987) Delignification of an unbleached hardwood kraft pulp byPhanerochaete chrysosporium. Appl Microbiol Biotech 25:484–490

    CAS  Article  Google Scholar 

  3. 3.

    Katagiri N, Tsutsumi Y, Nishida T (1995) Correlation of brightness with cumulative enzyme activity related to lignin biodegradation during biobleaching of kraft pulp by white rot fungi in the solid-state fermentation system. Appl Environ Microbiol 61:617–622

    CAS  PubMed  PubMed Central  Google Scholar 

  4. 4.

    Paice MG, Jurasek L, Ho C, Bourbonnais R, Archibald F (1989) Direct biological bleaching of hardwood kraft pulp with fungusCoriolus versicolor. TAPPI J 72(5):217–221

    CAS  Google Scholar 

  5. 5.

    Kirkpatrick N, Reid ID, Ziomek E, Paice MG (1990) Biological bleaching of hardwood kraft pulp usingTrametes (Corilus) versicolor immobilized in polyurethane form. Appl Microbiol Biotech 33:105–108

    CAS  Article  Google Scholar 

  6. 6.

    Fujita K, Kondo R, Sakai K, Kashino Y, Nishida T, Takahara Y (1991) Biobleaching of kraft pulp with whiterot fungus IZU-154. TAPPI J 74(11):123–127

    CAS  Google Scholar 

  7. 7.

    Murata S, Kondo R, Sakai K, Kashino Y, Nishida T, Takahara Y (1992) Chlorine-free bleaching process of kraft pulp using treatment with the fungus IZU-154. TAPPI J 75(12):91–94

    CAS  Google Scholar 

  8. 8.

    Kondo R, Harazono K, Sakai K (1994) Bleaching of hardwood kraft pulp with manganese peroxidase secreted fromPhanerochaete sordida YK-624. Appl Environ Microbiol 60:4359–4363

    CAS  PubMed  PubMed Central  Google Scholar 

  9. 9.

    Iimori T, Kaneko R, Yoshikawa H, Machida M, Yoshioka H, Murakami K (1994) Screening of pulp-bleaching fungi and bleaching activity of newly isolated fungus SKB-1152. Mokuzai Gakkaishi 40:733–737

    CAS  Google Scholar 

  10. 10.

    Wariishi H, Valli K, Gold MH (1991) In vitro depolymerization of lignin by manganese peroxidase ofPhanerochaete chrysosporium. Biochem Biophys Res Commun 176:269–276

    CAS  Article  PubMed  Google Scholar 

  11. 11.

    Hammel KE, Moen MA (1991) Depolymerization of a synthetic lignin in vitro by lignin peroxidase. Enzyme Microb Technol 13:15–18

    CAS  Article  Google Scholar 

  12. 12.

    Kawai S, Ohashi H, Hirai T, Okuyama H, Higuchi T (1993) Degradation of syringyl lignin model polymer by laccase ofCorilus versicolor. Mokuzai Gakkaishi 39:98–102

    CAS  Google Scholar 

  13. 13.

    Paice MG, Reid ID, Bourbonnaus R, Archibald FS, Jurasek L (1993) Manganese peroxidase, produced byTrametes versicolor during pulp bleaching, demethylates and delignifies kraft pulp. Appl Environ Microbiol 59:260–265

    CAS  PubMed  PubMed Central  Google Scholar 

  14. 14.

    Kaneko R, Iimori T, Yoshikawa H, Machida M, Yoshioka H, Murakami K (1994) A possible role of manganese peroxidase during biobleaching by the pulp bleaching fungus SKB-1152. Biosci Biotech Biochem 58:1817–1818

    Google Scholar 

  15. 15.

    Hirai H, Kondo R, Sakai K (1994) Screenig of lignin-degrading fungi and their ligninolytic activities during biological bleaching of kraft pulp. Mokuzai Gakkaishi 40:980–986

    CAS  Google Scholar 

  16. 16.

    Iimori T, Kaneko R, Miyawaki S, Machida M, Murakami K (1996) Oxidation of veratryl alcohol and detection of lignin peroxidase activity in biobleaching of oxygen-bleached kraft pulp by SKB-1152. Mokuzai Gakkaishi 42:862–867

    CAS  Google Scholar 

  17. 17.

    Bourbonnaus R, Paice MG (1996) Enzymatic delignification of kraft pulp using laccase and a mediator. TAPPI J 79(6):199–204

    Google Scholar 

  18. 18.

    Call HP, Mücke I (1995) The laccase-mediator system (LMS) — a new concept. In: proceedings of the 6th international conference on biotechnology in the pulp and paper industry, pp 27–32

  19. 19.

    Arbeloa M, Leclerc J, Goma G, Pommier JC (1990) An evaluation of lignin peroxidases to improve pulps. TAPPI J 73:215–221

    Google Scholar 

  20. 20.

    Harazono K, Kondo R, Sakai K, Tsuchikawa K (1996) Application of ligninolytic enzymes to bleaching of kraft pulp. III. Bleaching of kraft pulp with manganese peroxidase combined with lignin peroxidase or xylanase (in Japanese). Jpn TAPPI J 50:1292–1298

    CAS  Article  Google Scholar 

  21. 21.

    Tien M, Kirk TK (1988) Lignin peroxidase ofPhanerochate chrysosporium. Methods Enzymol 161:238–249

    CAS  Article  Google Scholar 

  22. 22.

    Tonon F, Odier E (1988) Influence of veratryl alcohol and hydrogen peroxide on ligninase activity and ligninase production byPhanerochaete chrysosporium. Appl Environ Microbiol 54:462–472

    Google Scholar 

  23. 23.

    Jáger A, Crown E (1985) Production of ligninases and degradation of lignin in agitated submerged cultures ofPhanerochate chrysosporium. Appl Environ Microbiol 50:1274–1278

    PubMed  PubMed Central  Google Scholar 

  24. 24.

    Kuwahara M, Asada Y, Kimura Y, Ackage M (1987) Isolation of mutants of a lignin-degrading basidiomycete,Phanerochaete chrysosporium, that produce lignin peroxidase in high nitrogen cultures. Mokuzai Gakkaishi 33:821–823

    Google Scholar 

  25. 25.

    Tien M, Kirk TK (1984) Lignin-degrading enzyme fromPhanerochaete chrysosporium: purification, characterization, and catalytic properties of a unique H2O2-requiring oxygenase. Proc Natl Acad Sci USA 81:2280–2284

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  26. 26.

    Gellerstedt G, Pranda J, Lindfors EL (1994) Structural and molecular properties of residual birch kraft lignin. J Wood Chem Technol 14:467–482

    CAS  Article  Google Scholar 

  27. 27.

    Khindaria A, Barr DP, Aust SD (1995) Lignin peroxidases can also oxidize manganese. Biochemistry 34:7773–7779

    CAS  Article  PubMed  Google Scholar 

  28. 28.

    Srebotnik E, Messner K (1990) Accessibility of sulfite pulp to lignin peroxidase and marker proteins with respect to enzymatic bleaching. J Biotechnol 13:199–210

    CAS  Article  Google Scholar 

  29. 29.

    Kaneko R, Iimori T, Miyawaki S, Machida M, Murakami K (1995) Biobleaching with manganese peroxidase purified from the pulp bleaching fungus SKB-1152. Biosci Biotechnol Biochem 59:1584–1585

    CAS  Article  Google Scholar 

  30. 30.

    Gellerstedt G, Gustafsson K, Lindfors EL (1986) Structural changes in lignin during oxygen bleaching. Nordic Pulp Paper Res J 1(3):14–17

    CAS  Article  Google Scholar 

  31. 31.

    Francis RC, Lai YZ, Dence CW, Alexander TC (1991) Estimating the concentration of phenolic hydroxyl groups in wood pulps. TAPPI J 74(9):219–224

    CAS  Google Scholar 

  32. 32.

    Hosoya S, Shimada K, Taneda H, Channg HM (1991) The behavior of pulping-resistant structure in lignin during bleaching. In: Proceedings of 6th ISWPC, vol 1, pp 563–569

Download references

Author information

Affiliations

Authors

Corresponding author

Correspondence to Takeshi Iimori.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Iimori, T., Miyawaki, S., Machida, M. et al. Biobleaching of unbleached and oxygen-bleached hardwood kraft pulp by culture filtrate containing manganese peroxidase and lignin peroxidase fromPhanerochaete chrysosporium . J Wood Sci 44, 451–456 (1998). https://doi.org/10.1007/BF00833409

Download citation

Key words

  • Biological bleaching
  • Enzyme bleaching
  • Lignin peroxidase
  • Manganese peroxidase
  • Phanerochaete chrysosporium