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Structure of xylan from culms of bamboo grass (Sasa senanensis Rehd.)

Abstract

Xylan prepared from culms of kumaizasa (Sasa senanensis Rehd.), a representative species of bamboo grass, was hydrolyzed withβ-xylanase ofStreptomyces olivaceoviridis E-86. Four arabinoxylo-oligosaccharides and two glucuronoxylo-oligosaccharides were isolated from the enzymatic hydrolysate of the xylan by chromatography on a charcoal column, a Dowex 1-x8 column, a Toyo-pearl HW-40S column, and a LiChrospher 100 NH2 column and on preparative paper chromatography. The results of the structural analyses of the saccharides showed that the isolated oligosaccharides had the structures of 32-α-l-arabinofuranosyl-xylobiose, 32-α-l-arabinofuranosyl-xylotriose, 32-α-[β-d-xylopyranosyl-(1 → 2)-l-arabinofuranosyl]-xylobiose, 33-α-[β-d-xylopyranosyl-(1 → 2)-l-arabinofuranosyl]-xylotriose, 23-α-4-O-methyl-d-glucuronosyl-xylotriose, and 23-α-d-glucuronosyl-xylotriose. From the structural analysis of the oligosaccharides derived from the xylan, kumaizasa xylan was concluded to be a kind of arabinoglucuronoxylan having not only stubs of singlel-arabinose and singled-glucuronic acid but also stubs of disaccharide units such asβ-d-xylopyranosyl-(1 → 2)-l-arabinofuranose.

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Correspondence to Shigeki Yoshida.

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Yoshida, S., Kuno, A., Saito, N. et al. Structure of xylan from culms of bamboo grass (Sasa senanensis Rehd.). J Wood Sci 44, 457–462 (1998). https://doi.org/10.1007/BF00833410

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Key words

  • Bamboo grass
  • Sasa senanensis Rehd
  • Xylan Xylanase
  • Xylooligosaccharide