Skip to main content

Official Journal of the Japan Wood Research Society

  • Original Article
  • Published:

In vitro screening of angiotensin I-converting enzyme inhibitors from Japanese cedar (Cryptomeria japonica)

Abstract

Screening and isolation of angiotensin I-converting enzyme (ACE) inhibitors from Japanese cedar (Cryptomeria japonica) based on the in vitro ACE inhibitory assay were attempted. The ethanol extract from outer bark showed the highest inhibitory activity (IC50 is 16μg/ml) among 24 extracts prepared from roots, leaves, heartwood, sapwood, inner bark, and outer bark by successive extraction with four solvents. The fractionation of the outer bark ethanol extract followed by the bioassay resulted in the isolation of two strong ACE inhibitors, catechin and dimeric procyanidin B3. The bioassay of three flavan-3-ols including (+)-catechin and six flavones revealed that most of these compounds have high ACE inhibitory activity. The results suggest that the phenolic hydroxyl group at the C7 position and heterocyclic oxygen atom of these compounds are important for expressing the inhibitory activity.

References

  1. Nishida T, Hanamura Y, Tsutsumi Y (1995) Superoxide dismutase mimic and tyrosinase inhibitory activities of extractives from sugi (Cryptomeria japonica). Mokuzai Gakkaishi 41:522–524

    CAS  Google Scholar 

  2. Ondetti M, Rubin B, Cushman D (1977) Design of specific inhibitors of angiotensin-converting enzyme: a new class of orally active antihypersensitive agents. Science 196:441–447

    Article  CAS  PubMed  Google Scholar 

  3. Ondetti MA (1988) Structural relationship of angiotensin converting enzyme inhibitors to pharmacologic activity. Circulation (suppl I) 77:74–78

    Google Scholar 

  4. Hansen K, Nyman U, Smitt U, Adsersen A, Gudiksen L, Rajasekharan S, Pushpangadan P (1995) In vitro screening of traditional medicines for anti-hypertensive effect based on inhibition of the angiotensin converting enzyme. J Ethnopharmacol 48(1):43–51

    Article  CAS  PubMed  Google Scholar 

  5. Sendl A, Elbl G, Redl K, Breu W, Wagner H (1992) Comparative pharmacological investigations ofAllium ursinum andAllium sativum. Planta Med 58:1–7

    Article  CAS  PubMed  Google Scholar 

  6. Wagner H, Elbl G (1992) ACE-inhibitory procyanidins fromLespedeza capitata. Planta Med 58:297

    Article  CAS  PubMed  Google Scholar 

  7. Wagner H (1993) Leading structure of plant origin for drug development. J Ethnopharmacol 38:105–112

    Article  CAS  PubMed  Google Scholar 

  8. Chen C-H, Lin J-Y (1992) Inhibition of angiotensin-I-converting enzyme by tetrahydroxyxanthones isolated fromTripterospermum lanceolatum. J Nat Prod 55:691–695

    Article  CAS  PubMed  Google Scholar 

  9. Hansen K, Adsersen A, Christensen S, Jensen S, Nyman U, Smitt U (1996) Isolation of an angiotensin converting enzyme (ACE) inhibitor fromOlea europaea andOlea lancea. Phytomedicine 2:319–325

    Article  CAS  PubMed  Google Scholar 

  10. Hara Y, Matsuzaki T, Suzuki T (1987) Angiotensin I converting inhibiting activity of tea components (in Japanese). Nippon Nogeikagaku Kaishi 61:803–808

    Article  CAS  Google Scholar 

  11. Imamura H, Yasue M (1983) List of the extractives from woody plants. In: Imamura H (ed) Chemistry for wood utilization (in Japanese). Kyoritsu Shuppan, Tokyo, pp 324–399

    Google Scholar 

  12. Samejima M, Yoshimoto T (1979) Procyanidins from the inner bark of sugi (Cryptomeria japonica D. Don). Mokuzai Gakkaishi 25:671–677

    CAS  Google Scholar 

  13. Cheung HS, Cushman DW (1973) Inhibition of homogeneous angiotensin-converting enzyme of rabbit lung by synthetic venom peptides ofBothrops jararaca. Biochim Biophys Acta 293:451–463

    Article  CAS  PubMed  Google Scholar 

  14. Tsai B-S, Peach MJ (1977) Angiotensin homologs and analogs as inhibitors of rabbit pulmonary angiotensin-converting enzyme. J Biol Chem 252:4674–4681

    CAS  PubMed  Google Scholar 

  15. Hamburger M, Hostettmann K (1991) Bioactivity in plants: the link between phyrochemistry and medicine. Phytochemistry (Oxf) 30:3864–3874

    Article  CAS  Google Scholar 

  16. Tachibana S (1995) Utilization of biologically active substances in trees (in Japanese). Mokuzai Gakkaishi 41:967–977

    CAS  Google Scholar 

  17. Harborne JB (1989) Flavonoids. In: Powe JW (ed) Natural products of woody plants, vol. I. Springer, pp 533–570

  18. Ebel J, Hahlbrock K (1982) Biosynthesis. In: Harborne JB, Mabry TJ (ed) The flavonoids: advances in research. Chapman & Hall, New York, pp 641–679

    Chapter  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Yuji Tsutsumi.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tsutsumi, Y., Shimada, A., Miyano, A. et al. In vitro screening of angiotensin I-converting enzyme inhibitors from Japanese cedar (Cryptomeria japonica). J Wood Sci 44, 463–468 (1998). https://doi.org/10.1007/BF00833411

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00833411

Key words