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In vitro screening of angiotensin I-converting enzyme inhibitors from Japanese cedar (Cryptomeria japonica)

Journal of Wood Science volume 44, pages463–468(1998)Cite this article

Abstract

Screening and isolation of angiotensin I-converting enzyme (ACE) inhibitors from Japanese cedar (Cryptomeria japonica) based on the in vitro ACE inhibitory assay were attempted. The ethanol extract from outer bark showed the highest inhibitory activity (IC50 is 16μg/ml) among 24 extracts prepared from roots, leaves, heartwood, sapwood, inner bark, and outer bark by successive extraction with four solvents. The fractionation of the outer bark ethanol extract followed by the bioassay resulted in the isolation of two strong ACE inhibitors, catechin and dimeric procyanidin B3. The bioassay of three flavan-3-ols including (+)-catechin and six flavones revealed that most of these compounds have high ACE inhibitory activity. The results suggest that the phenolic hydroxyl group at the C7 position and heterocyclic oxygen atom of these compounds are important for expressing the inhibitory activity.

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Author information

Affiliations

  1. Faculty of Agriculture, Shizuoka University, 422-8529, Shizuoka, Japan

    Yuji Tsutsumi, Ayako Shimada, Akiko Miyano & Tomoaki Nishida

  2. Faculty of Bioresources, Mie University, 514-8507, Tsu, Japan

    Tohru Mitsunaga

Authors
  1. Yuji Tsutsumi
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  2. Ayako Shimada
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  3. Akiko Miyano
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  4. Tomoaki Nishida
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  5. Tohru Mitsunaga
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Correspondence to Yuji Tsutsumi.

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Tsutsumi, Y., Shimada, A., Miyano, A. et al. In vitro screening of angiotensin I-converting enzyme inhibitors from Japanese cedar (Cryptomeria japonica). J Wood Sci 44, 463–468 (1998). https://doi.org/10.1007/BF00833411

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Key words

  • ACE inhibitor
  • Cryptomeria japonica
  • Catechin
  • Procyanidin B3
  • Flavonoid