Skip to main content

Official Journal of the Japan Wood Research Society

Improvement of penetrability of sugi wood by impregnation of bacteria using sap-flow method


The sap flow method of wood impregnation was conducted to aid the movement of bacteria through the living tree, thereby accelerating their distribution through wood within a short time. When log-pond water containing mixed species of bacteria were introduced in the living trees by butt-end dipping and then laid horizontally for 6 months, bacteria could be delivered by sap flow vertically through the sapwood tracheids up to the high portions from the butt-end of trees; they could be detected in the ray parenchymal cells. The sap-flow method was assumed to deliver the bacteria to sapwood and heartwood at high levels of standing sugi (Cryptomeria japonica D. Don) trees. Degradation of the pit membranes was observed even at more than 3 m upward from the butt-end after the treatment in sapwood, as well as around the butt-end of the trees. The uptake of the aqueous dye solutions in sapwood of the treated logs were about eight times more than those of control specimens after 8h.


  1. Furuyama Y, Kanagawa Y, Hayashi K (1995) Estimation of free water flow in wood during drying by permeability coefficient. Mokuzai Gakkaishi 41:710–717

    Google Scholar 

  2. Fujisawa Y, Ohta S, Nishimura K, Toda T, Tajima M (1995) Variation in moisture contents of heartwood among clones and test stands in sugi (Cryptomeria japonica). Mokuzai Gakkaishi 41:249–255

    Google Scholar 

  3. Krzyzewski J (1979) Ponding as an industrial tool for improving the permeability of white spruce poles to creosote. Forintec Canada CO., Technical Report 516ER (Ottawa), pp 1–15

  4. Kobayashi Y, Iida I, Imamura Y, Watanabe U (in press) Drying and anatomical characteristics of sugi wood attacked by bacteria during pond storage. Mokuzai Gakkaishi.

  5. Iida I, Morioka S (1990) Permeability of dye solution into wood by sap-flow method. Mokuzai Hozon 16:30–37

    Article  Google Scholar 

  6. Clausen CA (1996) Bacterial associations with decay wood: a review. Int Biodet Biodeg 37:101–107

    Article  Google Scholar 

  7. Johnson BR, Goivik LR (1970): Effect ofTrichoderma viride and a contaminating bacterium on microstructure and permeability of loblolly pine and Douglas-fir. Proc Am Wood Pres Assoc 66:1–7

    Google Scholar 

  8. Iida I, Imamura Y (1994) On the method of penetration of chemical solutions into wood using compression, microwave heating and sapflow method. Mokuzai Hozon 20:312–317

    Article  Google Scholar 

  9. Yoshimoto T (1983) Relationship between bacteria and wood extractives. In: Science for wood technology. Kyoritu Shuppan, Tokyo, pp 159–160

    Google Scholar 

  10. Kobayashi Y, Iida I, Imamura Y (1994) An acceleration of water movement in wood using pit-perforation effects by bacteria. In: Proceedings of 4th IUFRO International Wood Drying Conference, Rotorua, pp 458–459

Download references

Author information

Authors and Affiliations


Corresponding author

Correspondence to Yoshinori Kobayashi.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Kobayashi, Y., Iida, I., Imamura, Y. et al. Improvement of penetrability of sugi wood by impregnation of bacteria using sap-flow method. J Wood Sci 44, 482–485 (1998).

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI:


  • Sugi (Cryptomeria japonica)
  • Sap-flow method
  • Bacterial degradation
  • Penetrability