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Reaction of manganese peroxidase ofBjerkandera adusta with synthetic lignin in acetone solution

Abstract

The reaction of manganese peroxidase (MnP) of the white-rot fungusBjerkandera adusta with synthetic lignin dehydrogenation polymer, DHP) in acetone medium was investigated. Gel-permeation chromatography of the DHP treated by MnP demonstrated depolymerization of syringyl DHP in the reaction mixture containing 70% acetone; moreover, concomitant repolymerization occurred to give highly polymerized products. Guaiacyl DHP was only repolymerized by MnP in the same acetone solution without giving degradation products. Addition of ascorbic acid to reaction mixtures containing acetone resulted in preferential depolymerization of syringyl DHP.

References

  1. 1.

    Sarkanen KV, Ludwig CH (1971) Lignins: occurrence, formation, structure and reactions. J Wiley, New York, pp 43–89

  2. 2.

    Gold MH, Wariishi H, Valli K (1989) Extracellular peroxidases involved in lignin degradation by the white rot basidiomycetePhanerochaete chrysosporium. In: Whitaker RJ, Sonnet PE (eds) Biocatalysis in agricultural biotechnology. ACS Symposium Series 389. American Chemical Society, Washington D.C., pp 127–140

  3. 3.

    Hammel KE (1989) Organopollutant degradation by ligninolytic fungi. Enzyme Microb Technol 11:776–777

  4. 4.

    Thurston CF (1994) The structure and function of fungal laccases. Microbiology 140:19–26

  5. 5.

    Umezawa T, Higuchi T (1989) Cleavages of aromatic ring andβ-O-4 bond of synthetic lignin (DHP) by lignin peroxidase. FEBS Lett 242:325–329

  6. 6.

    Kondo R, Iimori T, Imamura H, Nishida T (1990) Polymerization of DHP and depolymerization of DHP-glucoside by lignin peroxidase. J Biotechnol 13:181–188

  7. 7.

    Hammel KE, Moen MA (1991) Depolymerization of a synthetic lignin in vitro by lignin peroxidase. Enzyme Microb Technol 13:15–18

  8. 8.

    Wariishi H, Valli K, Gold MH (1991) In vitro depolymerization of lignin by manganese peroxidase ofPhanerochaete chrysosporium. Biochem Biophys Res Commun 176:269–275

  9. 9.

    Hammel KE, Jensen KA Jr, Mozuch MD, Landucci LL, Tien M, Pease EA (1993) Ligninolysis by a purified lignin peroxidase. J Biol Chem 268:12274–12281

  10. 10.

    Kawai S, Ohashi H, Hirai T, Okuyama H, Higuchi T (1993) Degradation of syringyl lignin model polymer by laccase ofCoriolus versicolor. Mokuzai Gakkaishi 39:98–102

  11. 11.

    Bao W, Fukushima Y, Jensen KA Jr, Moen MA, Hammel KE (1994) Oxidative degradation of non-phenolic lignin during lipid peroxidation by fungal manganese peroxidase. FEBS Lett 354:297–300

  12. 12.

    Dordick JS, Marletta MA, Klibanov AM (1986) Peroxidases depolymerization lignin in organic media but not in water. Proc Natl Acad Sci USA 83:6255–6257

  13. 13.

    Lewis NG, Razal RA, Yamamoto E (1987) Lignin degradation by peroxidase in organic media: a reassessment. Proc Natl Acad Sci USA 84:7925–7927

  14. 14.

    Yoshida S, Watanabe T, Honda Y, Kuwahara M (1996) Reaction of lignin peroxidase ofPhanerochaete chrysosporium in organic solvents. Biosci Biotech Biochem 60:711–713

  15. 15.

    Vazquez-Duhalt R, Westlake DWS, Fedorak PM (1994) Lignin peroxidase oxidation of aromatic compounds in systems containing organic solvents. Appl Environ Microbiol 60:459–466

  16. 16.

    Yoshida S, Watanabe T, Honda Y, Kuwahara M (1997) Effects of water-miscible organic solvents on the reaction of lignin peroxidase ofPhanerochaete chrysosporium. J Mol Catal B 2:243–251

  17. 17.

    Vazquez-Duhalt R, Westlake DWS, Fedorak PM (1995) Kinetics of chemically modified lignin peroxidase and enzymatic oxidation of aromatic nitrogen-containing compounds. Appl Microbiol Biotechnol 42:675–681

  18. 18.

    Yoshida S, Watanabe T, Honda Y, Kuwahara M (1996) Reaction of chemically modified lignin peroxidase ofPhanerochaete chrysosporium in water-miscible organic solvents. Biosci Biotech Biochem 60:1805–1809

  19. 19.

    Kimura Y, Asada Y, Kuwahara M (1990) Screening of basidiomycetes for lignin peroxidase genes using a DNA probe. Appl Microbiol Biotechnol 32:436–442

  20. 20.

    Kofujita H, Asada Y, Kuwahara M (1991) Alkyl-aryl cleavage of phenolicβ-O-4 lignin substructure model compound by Mn(II)peroxidase isolated fromPleurotus ostreatus. Mokuzai Gakkaishi 37:555–561

  21. 21.

    Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

  22. 22.

    Kirk TK, Croan S, Tien M, Murtagh KE, Farrell RL (1986) Production of multiple ligninases byPhanerochaete chrysosporium: effect of selected growth conditions and use of a mutant strain. Enzyme Microb Technol 8:27–32

  23. 23.

    Freudenberg K, Hübner HH (1952) Oxyzimtalkohole und ihre Dehydrierungs-polymerisate. Chem Ber 85:1181–1191

  24. 24.

    Tanahashi M, Higuchi T (1981) Dehydrogenative polymerization of monolignols by peroxidase and H2O2 in a dialysis tube. I. Preparation of highly polymerized DHPs. Wood Res 67:29–42

  25. 25.

    Tanahashi M, Higuchi T (1990) Effect of the hydrophobic regions of hemicelluloses on dehydrogenative polymerization of sinapyl alcohol. Mokuzai Gakkaishi 36:424–428

  26. 26.

    Schuerch C (1952) The solvent properties of liquids and their relation to the solubility, swelling, isolation and fractionation of lignin. J Am Chem Soc 74:5061–5067

  27. 27.

    Adler E (1977) Lignin chemistry — past, present and future. Wood Sci Technol 11:169–218

  28. 28.

    Haemmerli SD, Leisola MSA, Fiechter A (1986) Polymerization of lignins by ligninases fromPhanerochaete chrysosporium. FEMS Microbiol Lett 35:33–36

  29. 29.

    Wariishi H, Valli K, Gold MH (1989) Oxidative cleavage of a phenolic diarylpropane lignin model dimer by manganese peroxidase fromPhanerochaete chrysosporium. Biochemistry 28:6017–6023

  30. 30.

    Tuor U, Wariishi H, Schoemarker HE, Gold MH (1992) Oxidation of phenol arylglycerol β-aryl ether lignin model compounds by manganese peroxidase fromPhanerochaete chrysosporium: oxidative cleavage of an α-carbonyl model compound. Biochemistry 31:4986–4995

  31. 31.

    Conn EE, Stumpf PK, Bruening G, Doi H (1987) Outlines of biochemistry. J Wiley, New York, pp 165–211

  32. 32.

    Wariishi H, Valli K, Gold MH (1992) Manganese (II) oxidation by manganese peroxidase from the basidiomycetePhanerochaete chrysosporium. J Biol Chem 267:23688–23695

  33. 33.

    Kurek B, Monties B, Odier E (1990) Dispersion of lignin in water: characterization of the phenomenon and influence on lignin degradability. Holzforschung 44:407–414

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Correspondence to Masaaki Kuwahara.

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Yoshida, S., Chatani, A., Honda, Y. et al. Reaction of manganese peroxidase ofBjerkandera adusta with synthetic lignin in acetone solution. J Wood Sci 44, 486–490 (1998). https://doi.org/10.1007/BF00833415

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Key words

  • Lignin biodegradation
  • Manganese peroxidase
  • Bjerkandera adusta
  • Acetone