Skip to main content

Advertisement

Official Journal of the Japan Wood Research Society

Journal of Wood Science Cover Image
We’d like to understand how you use our websites in order to improve them. Register your interest.

Isolation and sequence analysis of the promoter and an allelic sequence of the iron-sulfur protein subunit gene from the white-rot fungusPleurotus ostreatus

Abstract

We have isolated a structural gene ofsdil, which encodes the iron-sulfur protein (Ip) subunit of succinate dehydrogenase (EC 1.3.99.1), from a white-rot basidiomycete,Pleurotus ostreatus. Here we report isolation of the promoter region ofsdil and an allelic sequence encoding the second-type cDNA fragment isolated in the former experiments. The nucleotide sequence analysis of the promoter region revealed the existence of putative CAAT and TATA boxes, which permits us to develop an expression system in this species. The Southern blot analysis and the restriction fragment length polymorphism assay using monokaryotic strains demonstrated that no family genes tosdil exist in the haploid genome ofP. ostreatus. Moreover, a genetic analysis to detect a linkage between thesdil genotypes and flutolanil resistance in the mutantP. ostreatus strains was also developed.

References

  1. 1.

    Kofujita H, Asada Y, Kuwahara M (1991) Alkyl-aryl cleavage of phenolicβ-O-4 lilgnin substructure model compound by Mn(II)peroxidase isolated fromPleurotus ostreatus. Mokuzai Gakkaishi 37:555–561

  2. 2.

    Casselton LA, De LFHA (1989) Heterologous gene expression in the basidiomycete fungusCoprinus cinereus. Curr Genet 16:35–40

  3. 3.

    Keon JPR, White GA, Hargreaves JA (1991) Isolation, characterization and sequence of a gene conferring resistance to the systemic fungicide carboxin from the maize smut pathogen,Ustilago maydis. Curr Genet 19:475–481

  4. 4.

    Broomfiled PLE, Hargreaves JA (1992) A single amino-acid change in the iron-sulphur protein subunit of succinate dehydrogenase confers resistance to carboxin inUstilago maydis. Curr Genet 22:117–121

  5. 5.

    Ackrell BAC, Kearney EB, Coles CJ, Singer TP, Beinert H, Wan YP, Folkers K (1977) Kinetics of the reoxidation of succinate dehydrogenase. Arch Biochem Biophys 182:107–117

  6. 6.

    Mowery PC, Ackrell BAC, Singer TP, White GA, Thorn GD (1976) Carboxins: powerful selective inhibitors of succinate oxidation in animal tissues. Biochem Biophys Res Commun 71:351–361

  7. 7.

    Ulrich JT, Mathre DE (1972) Mode of action of oxthaiin systemic fungicide. J Bacteriol110:628–632

  8. 8.

    White GA (1971) A potent effect of 1,4-oxathiin systemic fungicides on succinate oxidation by a particulate preparation fromUstilago maydis. Biochem Biophys Res Commun 44:1212–1219

  9. 9.

    Honda Y, Irie T, Atsuji M, Watanabe T, Kuwahara M (1996) Isolation and characterization ofPleurotus ostreatus mutant strains resistant to a carboxin-derived fungicide, flutolanil. Mycoscience 37:457–459

  10. 10.

    Irie T, Honda Y, Matsuyama T, Watanabe T, Kuwahara M (1998) Cloning and characterization of the gene encoding the iron-sulfur protein of succinate dehydrogenase fromPleurotus ostreatus. Biochim Biophys Acta 1396:27–31

  11. 11.

    Isegawa Y, Sheng Y, Sokawa J, Yamanishi Y, Nakagomi K, Ueda S (1992) Selective amplification of cDNA sequence from total RNA by cassette-ligation-mediated polymerase chain reaction (PCR): application to sequencing 6.5 kb genome segment of hantavirus strain B-1. Mol Cell Probes 6:467–474

  12. 12.

    Barnes WM (1994) PCR amplification of up to 35-kb DNA with high fidelity and high yield from lamda bacteriophage templates. Proc Natl Acad Sci USA 91:2216–2220

  13. 13.

    Nomura S, Inazawa J (1994) Sazanhaiburidaizâsyon (in Japanese). In: Mizutani T (ed) Datuaisotôpujikkenpurotokoru. Syuujyunsya, Tokyo, pp 35–43

  14. 14.

    Ciriasy M (1975) Genetics of alcohol dehydrogenase inSaccharomyces cervisiae. II. Two loci controlling synthesis of the glucoserepressible ADH II. Mol Gen Genet 138:157–164

  15. 15.

    Denis CL, Ciriacy M, Young ET (1981) A positive regulatory gene is required for accumulation of the functional messenger RNA for the glucose-repressible alcohol dehydrogenase fromSaccharomyces cerevisiae. J Mol Biol 148:355–368

  16. 16.

    Roos MH, Tielens AGM (1994) Differential expression of two succinate dehydrogenase subunit-B genes and a transition in energy metabolism during the development of the parasitic nematodeHaemonchus contortus. Mol Biochem Parasitol 66:273–281

Download references

Author information

Affiliations

Authors

Corresponding author

Correspondence to Masaaki Kuwahara.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Irie, T., Honda, Y., Matsuyama, T. et al. Isolation and sequence analysis of the promoter and an allelic sequence of the iron-sulfur protein subunit gene from the white-rot fungusPleurotus ostreatus . J Wood Sci 44, 491–494 (1998). https://doi.org/10.1007/BF00833416

Download citation

Key words

  • Succinate dehydrogenase
  • Iron-sulfur subunit
  • Gene cloning
  • White-rot basidiomycete
  • Pleurotus ostreatus