- Original Article
- Published:
Effect of water potential on fruit body formation ofLentinula edodes in sawdust-based substrate
Journal of Wood Science volume 45, pages 337–342 (1999)
Abstract
The influence of substrate water potential (ψ) on the growth and fruiting of three genotypes of shiitake (Lentinula edodes) was investigated. A slight reduction ofψ (−0.5MPa) stimulated mycelial and colony growth on liquid, agar, and sawdust-based substrates.L. edodes has been found to grow well at aψ around −0.5 MPa, which corresponds to a moisture content around 55%. A small decrease inψ at the final vegetative growth phase had positive effects on flush quantity. The substrateψ was significantly affected by the interaction between genotypes and spawn run time. Theψ of well-colonized mature substrate was −0.7MPa before and −4.OMPa after the fruiting. Theψ rose again to −0.7MPa during rapid absorbance of water by soaking, and this rise was repeated during the second and third flushes. It is suggested that the water-holding capacity of a substrate is related to culture maturity. Excellent water-providing capacity (higherψ) is expected in the substrate of well-matured cultures with a high density of mycelial colonization.
References
Boddy L (1983) The effect of temperature and water potential on growth rate of wood rotting basidiomycetes. Trans Br Mycol Soc 80:141–149
Eamus D, Jennings DH (1984) Determination of water, solute and turgor potentials of mycelium of various basidiomycete fungi causing wood decay. J Exp Bot 35:1782–1786
Tommerup IC (1984) Effect of soil water potential on spore germination by vesicular-arbuscular mycorrhizal fungi. Trans Br Mycol Soc 83:193–202
Kalberer PP (1987) Water potentials of casing and substrate and osmotic potentials of fruit bodies ofAgaricus bisporus. Scientia Hortic 32:175–182
Badham ER (1989) Influence of water potential on growth of shiitake mycelium. Mycologia 81:464–468
Ohga S (1990) Growth rate of mycelium of shiitake,Lentinus edodes, in relation to water potential of medium. J Fac Agr Kyushu Univ 34:413–420
Magan N, Challen MP, Elliot TJ (1995) Osmotic, matric and temperature effects on in vitro growth ofAgaricus bisporus andA. bitorquis strains. In: Elliott TJ (ed) Science and cultivation of edible fungi. Balkema, Rotterdam, pp 773–780
Donnelly DP, Boddy L (1997) Development of mycelial systems ofStropharia caerulea andPhanerochaete velutina on soil: effect of temperature and water potential. Mycol Res 101:705–713
Miles PG, Chang ST (1997) Mushroom biology. World Scientific Publishing, New York
Ohga S, Kitamoto Y (1997) Future of mushroom production and biotechnology. Food Rev Int 13:461–469
Royse DJ, Bahler CC (1986) Effects of genotype, spawn run time, and substrate formulation on biological efficiency of shiitake. Appl Environ Microbiol 52:1425–1427
Diehle DA, Royse DJ (1991) Effect of substrate heat treatment on biological efficiency (BE) and size of a selected line ofLentinula edodes. In: Maher MJ (ed) Science and cultivation of edible fungi. Balkema, Rotterdam, pp 517–521
Ohga S, Roozendael FV, Aspinwall M, Miwa M (1992) Yield and size response of the shiitake mushroom,Lentinus edodes, depending on incubation time on sawdust-based culture. Trans Mycol Soc Jpn 33:349–357
Donoghue JD, Denison WC (1995) Shiitake cultivation: gas phase during incubation influences productivity. Mycologia 87:239–244
Zhang YF, Molina FI (1995) Strain typing of Lentinula edodes by random amplified polymorphic DNA assay. FEMS Microbiol Lett 131:17–20
Clarke RW, Jennings DH, Coggins CR (1980) Growth of Serpula lacrimans in relation to water potential of substrate. Trans Br Mycol Soc 75:271–280
Wood DA, Goodenough PW (1977) Fruiting ofAgaricus bisporus: changes in extracellular enzyme activities during growth and fruiting. Arch Microbiol 114:161–165
Leatham GF (1985) Extracellular enzymes produced by the cultivated mushroomLentinus edodes during degradation of a lignocellulosic medium. Appl Environ Microbiol 50:859–867
Ohga S (1992) Comparison of extracellular enzyme activities among different strains of Lentinus edodes grown on sawdustbased cultures in relationship to their fruiting abilities. Mokuzai Gakkaishi 38:310–316
Ohga S (1995) Sawdust-based cultivation ofLentinus edodes and degree of culture maturity (in Japanese). Mushroom Sci Biotechnol 2:1–13
Wood DA, Allan M, Claydon N, Dudley K (1990) Regulation and properties ofAgaricus bisporus endocellulase. In: Proceedings of the International Union of the Microbiological Society Congress, Osaka, p 586
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Ohga, S. Effect of water potential on fruit body formation ofLentinula edodes in sawdust-based substrate. J Wood Sci 45, 337–342 (1999). https://doi.org/10.1007/BF00833500
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF00833500