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Journal of Wood Science

Official Journal of the Japan Wood Research Society

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Role of metal proteinases in the fruit-body formation ofHypsizygus marmoreus

Journal of Wood Science volume 44, pages 379–384 (1998)Cite this article

Abstract

We investigated the possible role of metal proteinase on the fruit-body formation ofHypsizygus marmoreus. The addition of a specific metal proteinase inhibitor, phosphoramidon, to the culture medium (10μg/ml) completely inhibited fruit-body formation. Metal proteinase activity in both the medium and the mycelia of this fungus increased markedly during vegetative mycelial growth, and activity was maximal 25 days after inoculation. When phosphoramidon was added to the culture medium during vegetative mycelial growth, the metal proteinase activity in the mycelium decreased to 56% of the control (without inhibitor) level. Isoelectric focusing analysis showed that two kinds of metal proteinases with a pl of 7.7 and 8.4, respectively, were obtained from 29-day-old mycelia. Uptake of phosphoramidon into the mycelia was confirmed as the result of inhibition of thermolysin activity by the mycelial extracts. The degree of inhibitor uptake into mycelia was about 2.0% and was independent of the initial concentration of the inhibitor administered. The addition of peptone and amino acids to medium treated with phosphoramidon resulted in fruit-body dry weight yields that were about 50% that of the control.

References

  1. Kinugawa K, Tanesaka E (1990) Changes in the rate of CO2 release from cultures of three basidiomycetes during cultivation. Trans Mycol Soc Jpn 31:489–500

    Google Scholar 

  2. Amano Y, Nishizawa K, Tokoo R, Matsuzawa T, Kaneda T (1992) Extracellular enzymes produced byLyophyllum ulmarium (Hypsizygus marmoreus) in commercial cultivation (in Japanese). Mokuzai Gakkaishi 38:411–416

    CAS  Google Scholar 

  3. Amano Y, Fukai Y, Nishizawa S, Matsuzawa T, Hayashi H, Kanda T, Ishihara M, Shimizu K (1990) Changes in medium constituents of some basidiomycete fungi in sawdust-based culture (in Japanese). In: Abstracts of 40th annual meeting of Japan Wood Research Society, Tsukuba, p 187

  4. Terashita T, Ro S, Yoshikawa K, Sbishiyama J (1995) Nutritional environment for mycelial growth and hydrolytic enzyme activities during the vegetative growth ofHypsizygus marmoreus (in Japanese). Mushroom Sci Biotech 2:15–20

    Google Scholar 

  5. Hartley BS (1960) Proteolytic enzymes. Annu Rev Biochem 29:45–73

    Article  CAS  PubMed  Google Scholar 

  6. Dohmae N, Hagashi K, Miki K, Tsumuraya Y, Hashimoto Y (1995) Purification and characterization of intracellular proteinases inPleurotus ostreatus fruiting bodies. Biosci Biotech Biochem 59:2074–2080

    Article  CAS  Google Scholar 

  7. Terashita T, Oda K, Kono M, Murao S (1985) Purification and some properties of metal proteinases fromLentinus edodes. Agric Biol Chem 49:2293–2300

    CAS  Google Scholar 

  8. Chao EE, Gruen HE (1987) Intracellular activity of mycelial proteinases during fruit-body development inFlammulina velutipes. Can J Bot 65:518–525

    Article  CAS  Google Scholar 

  9. Suda H, Aoyagi T, Takeuchi T, Umezawa H (1973) A thermolysin inhibitor produced by Actinomycetes: phosphoramidon. J Antibiot 26:621–623

    Article  CAS  PubMed  Google Scholar 

  10. Terashita T, Inoue T, Nakaie Y, Yoshikawa K, Shishiyama J (1997) Isolation and characterization of extra- and intra-cellular metal proteinases produced in the spawn-running process ofHypsizygus marmoreus. Mycoscience 38:243–245

    Article  Google Scholar 

  11. Endo Y (1962) Studies on proteases from thermophilic bacteria (in Japanese). Hakko Kogaku 40:346–353

    CAS  Google Scholar 

  12. Terashita T, Kono M (1987) Purification and some properties of carboxyl proteinases fromTricholonia matsutake (in Japanese). Trans Mycol Soc Jpn 28:245–256

    CAS  Google Scholar 

  13. Kitamoto Y, Matsumoto T, Hosoi N, Terashita T, Kono M, Ichikawa Y (1980) Nitrogen metabolism inFavolus arcularius: changes in cellular nitrogen compounds during development of the mycelium and fruit-bodies (in Japanese). Trans Mycol Soc Jpn 21:237–244

    CAS  Google Scholar 

  14. Terashita T, Kitamoto Y, Matsumoto T, Hosoi N, Ichikawa Y, Kono M (1984) Nitrogen metabolism inFavolus arcularius: changes in composition of free- and protein-amino acids during development of the mycelium and fruiting bodies (in Japanese). Trans Mycol Soc Jpn 25:187–198

    CAS  Google Scholar 

  15. Terashita T, Oda K, Kono M, Murao S (1981) Purification and some properties of carboxyl protemase in mycelium ofLentinus edodes. Agric Biol Chem 45:1929–1935

    CAS  Google Scholar 

  16. Murao S, Satoi S (1971) Studies on pepsin inhibitor (S-P1) fromStreptomyces naniwaensis. Part 1. Isolation of pepsin inhibitor producing microorganism. Agric Biol Chem 35:1477–1481

    CAS  Google Scholar 

  17. Yoshida H, Sasaki H, Fujimoto S, Sugahara T (1996) The chemical components in the vegetative mycelia of Basidiomycotina (in Japanese). Trans Mycol Soc Jpn 37:51–56

    CAS  Google Scholar 

  18. Terashita T, Kono M, Ueda M (1979) Effects of feeding of nitrogen sources on the fruit-body formation ofFavolus arcularius (in Japanese). Mem Fac Agric Kinki Univ 12:17–23

    Google Scholar 

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Authors and Affiliations

  1. Laboratory of Food Microbiology, Faculty of Agriculture, Kinki University, 631-8505, Nara, Japan

    Takao Terashita, Yoko Nakaie, Takaaki Inoue, Kentaro Yoshikawa & Jiko Shishiyama

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  1. Takao Terashita
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  2. Yoko Nakaie
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  3. Takaaki Inoue
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  4. Kentaro Yoshikawa
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  5. Jiko Shishiyama
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Terashita, T., Nakaie, Y., Inoue, T. et al. Role of metal proteinases in the fruit-body formation ofHypsizygus marmoreus . J Wood Sci 44, 379–384 (1998). https://doi.org/10.1007/BF01130451

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  • DOI: https://doi.org/10.1007/BF01130451

Key words

  • Hypsizygus marmoreus
  • Fruit-body
  • Phosphoramidon
  • Edible mushrooms
  • Metal proteinase