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Effects of applied gibberellins and uniconazole-P on gravitropism and xylem formation in horizontally positionedFraxinus mandshurica seedlings

Abstract

The present study deals with the effects of gibberellins (GA3 GA4) and uniconazole-P, an inhibitor of gibberellin biosynthesis, on negative gravitropism and xylem formation in the stems of horizontally positioned, 2-year-oldFraxinus mandshurica Rupr. var.japonica Maxim. seedlings. Each growth regulator (100 μg) dissolved in 5 μl acetone (50%) was applied to the basal node of the current shoot on May 24, 1995. The same treatment was repeated five times weekly until June 28. Five seedlings were used for each treatment. The seedlings were positioned horizontally 24h after the first application on May 25. Within 5 weeks the horizontal stem of control and GA-treated seedlings exhibited negative gravitropism. In contrast, the application of uniconazole-P inhibited negative gravitropic stem bending. The application of GAs increased the number of gelatinous fibers having thickened cell walls on the upper side of stems. The uniconazole-P application decreased xylem cell formation but did not inhibit the formation of gelatinous fibers. These results indicate that not only the differentiation of gelatinous fibers but also xylem increment is important in the negative gravitropism of horizontally positionedF. mandshurica seedlings. These results also suggest that GAs may be involved in xylem cell :formation rather than the differentiation of gelatinous fibers in this species.

References

  1. 1.

    Aloni R (1991) Wood formation in deciduous hardwood trees. In: Raghavendra AS (ed) Physiology of trees. Wiley, New York, pp 175–197

  2. 2.

    Little CHA, Savidge RA (1987) The role of plant growth regulators in forest tree cambial growth. Plant Growth Regul 6:137–169

  3. 3.

    Little CHA, Pharis RP (1995) Hormonal control of radial and longitudinal growth in the tree stem. In: Gartner BL (ed) Plant stems: physiology and functional morphology. Academic, San Diego, pp 281–319

  4. 4.

    Ridoutt BG, Pharis RP, Sands R (1995) Identification and quantification of cambial region hormones ofEucalyptus globulus. Plant Cell Physiol 36:1143–1147

  5. 5.

    Wang Q, Little CHA, Odén PC (1995) Effect of laterally applied gibberellin A4/7 on cambial growth and the level of indole-3-acetic acid inPinus sylvestris. Physiol Plant 95:187–194

  6. 6.

    Wang Q, Little CHA, Odén PC (1997) Control of longitudinal and cambial growth by gibberellins and indole-3-acetic acid in currentyear shoots ofPinus sylvestris. Tree Physiol 17:715–721

  7. 7.

    Cronshaw J, Morey PR (1968) The effect of plant growth substances on the development of tension wood in horizontally inclined stems ofAcer rubrum seedlings. Protoplasma 65:379–391

  8. 8.

    Morey PR, Cronshaw J (1968) Developmental changes in the secondary xylem ofAcer rubrum induced by gibberellic acid, various auxins 2,3,5-tri-iodobenzoic acid. Protoplasma 65:315–326

  9. 9.

    Timell TE (1986) Compression wood in gymnosperms, vol 1. Springer, Berlin Heidelberg, pp 1–7

  10. 10.

    Timell TE (1986) Compression wood in gymnosperms, vol 2. Springer, Berlin Heidelberg, pp 1245–1246

  11. 11.

    Denhard B, Feucht B (1971) Zur Reaktionsholzbildung beiPrunus avium. Holzforschung 25:169–174

  12. 12.

    Nakamura T, Saotome M, Ishiguro Y, Itoh R, Higurashi S, Hosono M, Ishii Y (1994) The effects of GA on weeping of growing shoots of the Japanese cherry,Prunus spachiana. Plant Cell Physiol 35:523–527

  13. 13.

    Saotome M, Ogiwara E, Omi T, Yokoyama T, Nakamura T (1995) Cellulose and lignin contents in GA-treated branches ofPrunus species (in Japanese). J Jpn Womens Univ Fac Sci 3:69–71

  14. 14.

    Nakamura T (1996) Induction of negative gravitorism by gibberellin in weeping Japanese cherry,Prunus spachiana. In: Plants in space biology. Institute of General Ecology, Tohoku University, pp 101–109

  15. 15.

    Baba K, Adachi K, Take T, Yokoyama T, Itoh T, Nakamura T (1995) Induction of tension wood in GA3 treated branches of the weeping type of Japanese cherry,Prunus spachiana. Plant Cell Physiol 36:983–988

  16. 16.

    Izumi K, Oshio H (1991) Effects of the growth retardant uniconazole-P on endogenous levels of hormones in rice plants. In: Takahashi N, Phinney BO, MacMillan J (eds) Gibberellins. Springer, New York, pp 330–338

  17. 17.

    Prodhan AKMA. Ohtani J, Funada R, Abe H, Fukazawa K (1995) Ultrastructural investigation of tension wood fibre inFraxinus mandshurica Rupr. var.japonica Maxim. Ann Bot 75:311–317

  18. 18.

    Oshio H, Tanaka S, Izumi K (1990) Development of uniconazole for a new plant growth retardant and studies on its mechanism of action and the practical uses (in Japanese). Chem Regul Plant 25(1):8–18

  19. 19.

    Blake TJ, Pharis RP, Reid DM (1980) Ethylene, gibberellins, auxin and the apical control of branch angle in a conifer,Cupressus arizonica. Planta 148:64–68

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Jiang, S., Furukawa, I., Honma, T. et al. Effects of applied gibberellins and uniconazole-P on gravitropism and xylem formation in horizontally positionedFraxinus mandshurica seedlings. J Wood Sci 44, 385–391 (1998). https://doi.org/10.1007/BF01130452

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Keywords

  • Tension Wood
  • Cell Wall Thickness
  • Basal Node
  • Cambial Activity
  • Xylem Cell