Skip to main content

Advertisement

Official Journal of the Japan Wood Research Society

Journal of Wood Science Cover Image
We’d like to understand how you use our websites in order to improve them. Register your interest.

Physiological considerations for efficient mycelial colonization of Philippine strains ofVolvariella volvacea

Abstract

The nutritional and physical requirements for the efficient mycelial colonization ofVolvariella volvacea (Bull. ex. Fr.). Singer were elucidated with the percentage mycelial colonization and density as references. This investigation was limited to the evaluation of two commercial strains (designated Vvc1 and Vvc2) and two wild strains (designated EAAC-0001 and EAAC-0002) ofV. volvacea from the Philippines with the aim of providing baseline data on their physiological requirements. The four strains ofV. volvacea had varying preferences for carbon. Vve1 preferred polysaccharides (starch and cellulose), whereas Vvc2 grew luxuriantly at a relatively rapid rate in sugar alcohol (sorbitol). The two wild strains preferred starch as a carbon source. In terms of nitrogen utilization, soytone, peptone, and glycine supported efficient mycelial colonization of the four strains. The vitamin utilization test revealed that ascorbic acid, calcium pantothenate, and biotin are good sources. The mycelial growth performances of the strains were also evaluated on six dehydrated mycological media. Efficient colonization of Vvc1, Vvc2, and EAAC-0002 with dense mycelial growth was noted in mycological agar. EAAC-0001, on the other hand, grew more efficiently in malt extract agar. The Philippine strains ofV. volvacea grew luxuriantly when incubated at 35°C and pH 8.0 under dark and sealed conditions. Moreover, the relatively higher moisture content (70%) of the oolong tea leaf formulation favorably stimulated efficient mycelial colonization. Under optimum physiological conditions, Vvc1, Vvc2, and EAAC-0002 were fast-growing strains, whereas EAAC-0001 was a moderately growing type.

References

  1. 1.

    Chang ST (1993) Biology and cultivation technology ofVolvariella volvacea. In: Chang ST, Boswell JA, Chin S (eds) Mushroom biology and mushroom products. Chinese University Press, Hong Kong, pp 73–83

  2. 2.

    Khan SM, Haq R, Dogar MA (1991) Some studies on the cultivation of Chinese mushroom (Volvariella volvacea (Fr.) Singer) on sugarcane industrial by-products. In: Maher MJ (ed) Science and cultivation of edible fungi. A.A. Balkema Publishers/Rotterdam, pp 579–584

  3. 3.

    Quimio TH (1990) The mushroom industry in the Philippines. Philippine Agric 73:323–331

  4. 4.

    Quimio TH (1993) Indoor cultivation of the straw mushroom. Mushroom Res 2 (2):87–90

  5. 5.

    Reyes RG, Abella EA (1993) Efficient utilization of agricultural wastes through mushroom production in the central Luzon region, Philippines. In: Abstracts of the First International Conference on Mushroom Biology and Mushroom Products, Hong Kong, p 110

  6. 6.

    Raudaskoski M, Viitanen H (1982) Effect of aeration and light on fruit body induction inSchizophyllum commune. Trans Br Mycol Soc 78:89–96

  7. 7.

    Torres-Lopez RI, Hepperly PR (1986) Nutritional influences of Volvariella volvacea grown in Puerto Rico. II. Vitamins, oils and pH. In: Wuest PJ, Royse DJ, Beelman RB (eds) Cultivating edible fungi: development in crop science 10. Elsevier, Amsterdam, pp 41–51

  8. 8.

    Gardner WH (1986) Moisture content. In: Klute A (ed) Methods of soil analysis. Part I. Physical and mineralogical methods, 2nd edn. Soil Science Society of America, Wisconsin, pp 493–544

  9. 9.

    Ofosu-Asiedu A, Schmidt O, Liese W (1984) Growth studies ofVolvariella volvacea for cultivation on wood waste. Mater Organ 19:241–251

  10. 10.

    Chakravarty DK, Mallick AK (1979) Studies on the physiology of mushroom fungi,Volvariella displasia andV. esculeuta. Ind J Mycol Plant Pathol 9:9–2

  11. 11.

    Fasidi IO, Jonathan SG (1994) Growth requirements ofVolvariella esculeuta (Mass) Singer, a Nigerian edible mushroom. Chem Mikrobiol Technol Lebensm 16(5/6):151–156

  12. 12.

    Chang ST (1978)Volvariella volvacea. In: Chang ST, Hayes WA (eds) The biology and cultivation of edible mushrooms. Academic Press, San Diego, pp 573–600

  13. 13.

    Vedder PJC (1991) The biggest mushroom farm in the world. In: Maher MJ (ed) Science and cultivation of edible fungi. A.A.Balkema Publishers/Rotterdam, pp 813–819

  14. 14.

    Chang ST (1972) The Chinese mushroom (Volvariella volvacea): morphology, cytology, genetics, mutrition and cultivation. Chinese University Press, Hong Kong, pp 1–99

  15. 15.

    Sik Seo G, Chull Shin G, Otani H, Kodama M, Kohmoto K (1995) Formation of atypical structures inGanoderma lucidum isolates on a nutrient agar medium. Mycoscience 36:1–7

  16. 16.

    Chang ST, Miles PG (1989) Edible mushrooms and their cultivation. Boca Raton, CRC Press, pp 225–251

  17. 17.

    Eguchi F, Yoshimoto H, Yoshimoto T, Higaki M (1994) Physiological factors affecting the mycelial growth ofAgaricus blazei. Mokuzai Gakkaishi 40:666–671

  18. 18.

    Kurtzman RH Jr, Ho YC (1982) Physiological considerations for cultivation ofVolvariella mushrooms. In: Chang ST, Quimio TH (eds) Tropical mushrooms: biological nature and cultivation methods. Chinese University Press, Hong Kong, pp 139–166

Download references

Author information

Affiliations

Authors

Corresponding author

Correspondence to Renato G. Reyes.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Reyes, R.G., Eguchi, F., Iijima, T. et al. Physiological considerations for efficient mycelial colonization of Philippine strains ofVolvariella volvacea . J Wood Sci 44, 408–413 (1998). https://doi.org/10.1007/BF01130456

Download citation

Key words

  • Mushroom nutrition
  • Mushroom physiology
  • Mycelial growth
  • Volvariella volvacea