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Journal of Wood Science

Official Journal of the Japan Wood Research Society

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Isolation and characterization of a novel anionic peroxidase cDNA found in poplar (Populus nigra) suspension cultured cells

Journal of Wood Science volume 47, pages 165–170 (2001)Cite this article

Abstract

A cDNA clone fromPopulus nigra L. var.italica Koehne, denoted PCY2-6, coding for an anionic peroxidase has been isolated, cloned, and characterized. PCY2-6 is 1160 bp long; and its deduced product, PnC26, contains 343 amino acid residues. The mature protein has a calculated isoelectric point of 4.09. The protein contains two motifs typical of peroxidase and 10 potentialN-glycosylation sites. PnC26 is therefore classified as an anionic peroxidase. The mRNA of the PCY2-6 gene family was detected in immature and mature leaves and in two parts of current-year stems: the shoot tip and the older stem. The mRNA of PCY2-6 gene family was found to localize in the phloem and cortex of the current-year stems. We therefore conclude that expression of the PCY2-6 gene family is related to bark development.

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Authors and Affiliations

  1. Iwate Biotechnology Research Center, Kitakami, 024-0003, Iwate, Japan

    Yoshiaki Kato

  2. Graduate School of Agriculture, Hokkaido University, Sapporo, 060-8589, Hokkaido, Japan

    Akira Ando, Kiyoshi Miura & Yutaka Tamai

  3. Biological Resources Division, Japan International Research Center for Agricultural Sciences (JIRCAS), Tsukuba, 305-8686, Ibaraki, Japan

    Yoh Sakuma

  4. Hokkaido Forest Products Research Institute, Asahikawa, 071-0181, Hokkaido, Japan

    Tomonori Azuma

  5. Graduate School of Agriculture, Kyoto University, 606-8502, Kyoto, Japan

    Keiji Takabe

Authors
  1. Yoshiaki Kato
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  2. Yoh Sakuma
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  3. Tomonori Azuma
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  4. Akira Ando
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  5. Kiyoshi Miura
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  7. Yutaka Tamai
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Correspondence to Yoshiaki Kato.

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Kato, Y., Sakuma, Y., Azuma, T. et al. Isolation and characterization of a novel anionic peroxidase cDNA found in poplar (Populus nigra) suspension cultured cells. J Wood Sci 47, 165–170 (2001). https://doi.org/10.1007/BF01171217

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  • DOI: https://doi.org/10.1007/BF01171217

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