Skip to main content
Journal of Wood Science

Official Journal of the Japan Wood Research Society

Download PDF

Polymorphism analysis of Fagaceae and DNA-based identification ofFagus species grown in Japan based on therbcL gene

Journal of Wood Science volume 45, pages 183–187 (1999)Cite this article

Abstract

Fagaceae species in Japan were identified by restriction fragment length polymorphism (RFLP) and sequence comparison of a region ofrbcL. Of nine restriction endonucleases used for digestion, three (MspI,RsaI,HaeIII) produced different restriction patterns in Fagaceae. Digestion byMspI yielded four patterns: Fagus species,Castanea crenata, Pasania glabra, and others. Digestion byRsaI andHaeIII afforded two patterns:Fagus species and others. These facts indicate thatCastanea crenata andPasania glabra can be identified byMspI restriction patterns ofrbcL. Sequence comparison of a region of therbcL gene among 20 species of Fagaceae showed that: (1) they could be divided into seven groups; (2) there is a site mutation betweenFagus crenata andF. japonica. The latter indicates that the wood of both Fagus species are identifiable at the species level, which is not the case using conventional methods. This result indicates the possibility of wood identification based on DNA polymorphism in Fagaceae at the intrageneric level.

References

  1. 1.

    Wheeler EA, Baas P, Gasson PE (ed) (1989) LAWA list of microscopic features for hardwood identification. LAWA Bull 10:219–332

    Google Scholar 

  2. 2.

    Sudo S (1959) Identification of Japanese hardwoods (in Japanese). Bull Gov For Exp Station 118:1–138

    Google Scholar 

  3. 3.

    Saiki RK, Scharf S, Faloona F, Mullis KB, Horn GT, Erlich HA, Arnheim N (1985) Enzymatic amplification ofβ-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science 230:1350–1354

    CAS  Article  PubMed  Google Scholar 

  4. 4.

    Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, Horn GT, Mullis KB, Erlich HA (1988) Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239:487–491

    CAS  Article  PubMed  Google Scholar 

  5. 5.

    Bousquet J, Strauss SH, Li P (1992) Complete congruence between morphological andrbcL-based molecular phylogenies in birches and related species (Betulaceae). Mol Biol Evol 9:1076–1088

    CAS  PubMed  Google Scholar 

  6. 6.

    Xiang QY, Soltis DE, Morgan DR, Soltis PS (1993) Phylogenetic relationship ofCornus L. sensu lato and putative relatives inferred fromrbcL sequence data. Ann Mo Bot Garden 80:723–734

    Article  Google Scholar 

  7. 7.

    Martin PG, Dowd JM (1993) Using sequences ofrbcL to study phylogeny and biogeography ofNothofagus species. Aust Syst Bot 6:441–447

    Article  Google Scholar 

  8. 8.

    Gunter LE, Kochert G, Giannasi DE (1994) Phylogenetic relationship of the Juglandaceae. Plant Syst Evol 192:11–29

    Article  Google Scholar 

  9. 9.

    Ueda K, Kosuge K, Tobe H (1997) A molecular phylogeny of Celtidaceae and Ulmaceae (Urticales) based onrbcL nucleotide sequences. J Plant Res 110:171–178

    CAS  Article  Google Scholar 

  10. 10.

    Manos PS, Steele KP (1997) Phylogenetic analyses of “higher” Hamamelididae based on plastid sequence data. Am J Bot 84:1407–1419

    CAS  Article  PubMed  Google Scholar 

  11. 11.

    Plunkett GM, Soltis DE, Soltis PS (1997) Clarification of the relationship between Apiaceae and Araliaceae based onmatK andrbcL sequence data. Am J Bot 84:565–580

    CAS  Article  PubMed  Google Scholar 

  12. 12.

    Setoguchi H, Ono M, Doi Y, Koyama H, Tsuda M (1997) Molecular phylogeny ofNothofagus (Nothofagaceae) based on theatpB-rbcL intergenic spacer of the chloroplast DNA. J Plant Res 110:469–484

    CAS  Article  Google Scholar 

  13. 13.

    Shiraishi S, Watanabe A (1995) Identification of chloroplast genome betweenPinus densifiora SIEB. et ZUCC. andP. thunbergii PARL. based on the polymorphism inrbcL gene (in Japanese). J Jpn For Soc 77:429–436

    Google Scholar 

  14. 14.

    Watanabe A, Maeda H, Shiraishi S (1997) A simple identification of chloroplast genome types betweenPinus densiftora Sieb. et Zucc. andP. thunbergii Parl. using PCR-SSCP analysis (in Japanese). J Jpn For Soc 79:155–156

    Google Scholar 

  15. 15.

    Shiraishi S, Isoda K, Watanabe A, Kawasaki H (1996) DNA systematical study on theLarix relicted at Mt. Manokami, the Zao Mountains (in Japanese). J Jpn For Soc 78:175–182

    Google Scholar 

  16. 16.

    Maeda H, Shiraishi S (1997) An identification of chloroplast DNA haplotypes ofLarix kaempferi andL. gmelinii var.japonica using fluorescence-based PCR-SSCP analysis ofrbcL gene. J For Res 2:187–188

    Article  Google Scholar 

  17. 17.

    Elias TS (1971) The genera of Fagaceae in the southeastern United States. J Arnold Arb 52:159–195

    Article  Google Scholar 

  18. 18.

    Kitamura S, Murata G (1979) Coloured illustrations of woody plant of Japan (in Japanese), vol II. Hoikusya, Osaka

    Google Scholar 

  19. 19.

    Chase MW, Soltis DE, Olmstead RG, Morgan D, Les DH, Mishler BD, Duvall MR, Price RA, Hills HG, Qiu Y, Kron KA, Rettig JH, Conti E, Palmer JD, Manhart JR, Sytsma KJ, Michaels HJ, Kress WJ, Karol KG, Clark WD, Hedren M, Gaut BS, Jansen RK, Kim K, Wimpee CF, Smith IF, Furnier GR, Strauss SH, Xiang Q, Plunkett GM, Soltis PS, Swensen SM, Williams SE, Gadek PA, Quinn CJ, Eguiarte LE, Golenberg E, Learn GH, Graham SW, Barrett SCH, Dayanandan S, Albert VA (1993) Phylogenetics of seed plants: an analysis of nucleotide sequences from the plastid generbcL. Ann Moi Bot Gard 80:528–580

    Article  Google Scholar 

  20. 20.

    Murray MG, Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8:4321–4325

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  21. 21.

    Yamada H, Kawazu T, Ito K, Sbibata M (1996) Investigation on the identification of provenances in Eucalyptus using RAPD markers (in Japanese). DNA Polymorph 3:158–162

    Google Scholar 

  22. 22.

    Frascaria N, Maggia L, Michaud M, Bousquet J (1993) TherbcL gene sequence from chestnut indicates a slow rate of evolution in the Fagaceae. Genome 36:668–671

    CAS  Article  PubMed  Google Scholar 

Download references

Author information

Affiliations

  1. Wood Research Institute, Kyoto University, 611-0011, Uji, Kyoto, Japan

    Motonari Ohyama, Kei'ichi Baba & Takao Itoh

  2. Faculty of Agriculture, Kyusyu University, 812-8581, Fukuoka, Japan

    Susumu Shiraishi

Authors
  1. Motonari Ohyama
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kei'ichi Baba
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Takao Itoh
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Susumu Shiraishi
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Ohyama, M., Baba, K., Itoh, T. et al. Polymorphism analysis of Fagaceae and DNA-based identification ofFagus species grown in Japan based on therbcL gene. J Wood Sci 45, 183–187 (1999). https://doi.org/10.1007/BF01177723

Download citation

Key words

  • Wood identification
  • Fagaceae
  • rbcL