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Official Journal of the Japan Wood Research Society

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Reduction of ferric chelate caused by various wood-rot fungi


The reduction of ferric chelate caused by various wood-rot fungi was analyzed. Ferric chelate reductive activity was detected in cell-free extracts of seven wood-rot fungi:Phanerochaete chrysosporium, P. sordida YK-624,Ganoderma sp. YK-505,Coriolus versicolor, Bjerkandera adusta, Tyromyces palustris, andGloeophyllum trabeum. These fungi produced NADPH- or NADH-dependent ferric chelate reductive enzymes (or both) of different molecular weight. In the liquid culture ofP. sordida YK-624 andC. versicolor, a positive correlation was observed between extracellular MnP activity and intracellular NADPH-dependent ferric chelate reductive activity.


  1. 1.

    Raymond KN, Muller G, Matzanke BF (1984) Complexation of iron by siderophores: a review of their solution and structural chemistry and biological function. Top Curr Chem 123:49–1023

    CAS  Article  Google Scholar 

  2. 2.

    Wood PM (1988) The potential diagram for oxygen at pH 7. Biochem J 253:287–289

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  3. 3.

    Highley TL (1977) Requirements for cellulose degradation by a brown-rot fungus. Mater Org 12:25–36

    Google Scholar 

  4. 4.

    Kirk TK, Ibach R, Mozuch MD, Conner AH, Highley TL (1991) Characteristics of cotton cellulose depolymerized by a brownrotting fungus, by acid, or by chemical oxidants. Holzforschung 45:239–244

    CAS  Article  Google Scholar 

  5. 5.

    Hirai H, Kondo R, Sakai K (1997) A model system for NAD(P)Hdependent reduction of manganese dioxide mediated by ferrous chelate in white-rot fungusPhanerochaete sordida YK-624. Mokuzai Gakkaishi 43:247–253

    CAS  Google Scholar 

  6. 6.

    Page WJ, Huyer M (1984) Derepression of the Azotobacter vinelandii siderophore system, using iron-containing minerals to limit iron repletion. J Bacteriol 158:496–502

    CAS  PubMed  PubMed Central  Google Scholar 

  7. 7.

    Lesuisse E, Raguzzi F, Crichton R (1987) Iron uptake by the yeastSaccharomyces cerevisiae: involvement of a reduction step. J Gen Microbiol 133:3228–3236

    Google Scholar 

  8. 8.

    Georgatsou E, Alexandraki D (1994) Two distinctly regulated genes are required for theSaccharomyces cerevisiae. Mol Cell Biol 14:3065–3073

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  9. 9.

    Bao W, Renganathan V (1992) Cellobiose oxidase ofPhanerochaete chrysosporium enhances crystalline cellulose degradation by cellulases. FEBS Lett 302:77–80

    CAS  Article  PubMed  Google Scholar 

  10. 10.

    Bao W, Usha SN, Renganathan V (1993) Purification and characterization of cellobiose dehydrogenase, a novel extracellular hemoflavoenzyme from the white-rot fungusPhanerochaete chrysosporium. Arch Biochem Biophys 300:705–713

    CAS  Article  PubMed  Google Scholar 

  11. 11.

    Henriksson G, Pettersson G, Johansson G, Ruiz A, Uzcategui E (1991) Cellobiose oxidase from Phanerochaete chrysosporium can be cleaved by papain into two domains. Eur J Biochem 196:101–106

    CAS  Article  PubMed  Google Scholar 

  12. 12.

    Kremer SM, Wood PM (1992) Evidence that cellobiose oxidase fromPhanerochaete chrysosporium is primarily an Fe(III) reductase: kinetic comparison with neutrophil NADPH oxidase and yeast flavocytochrome b2. Eur J Biochem 205:133–138

    CAS  Article  PubMed  Google Scholar 

  13. 13.

    Brock B, Rieble S, Gold MH (1995) Purification and characterization of a 1,4-benzoquinone reductase from the basidiomycetePhanerochaete chrysosporium. Appl Environ Microbiol 61:3076–3081

    CAS  PubMed  PubMed Central  Google Scholar 

  14. 14.

    Brock BJ, Gold MH (1996) 1,4-Benzoquinone reductase from the basidiomycete Phanerochaete chrysosporium: spectral and kinetic analysis. Arch Biochem Biophys 331:31–40

    CAS  Article  PubMed  Google Scholar 

  15. 15.

    Stahl JD, Aust SD (1995) Properties of a transplasma membrane redox system ofPhanerochaete chrysosporium. Arch Biochem Biophys 320:369–374

    CAS  Article  PubMed  Google Scholar 

  16. 16.

    Hirai H, Kondo R, Sakai K (1998) NADPH-dependent ferrireductase produced by white-rot fungusPhanerochaete sordida YK-624. J Wood Sci 44:369–374

    CAS  Article  Google Scholar 

  17. 17.

    Hirai H, Kondo R, Sakai K (1994) Screening of lignin-degrading fungi and their ligninolytic enzyme activities during biological bleaching of kraft pulp. Mokuzai Gakkaishi 40:980–986

    CAS  Google Scholar 

  18. 18.

    Khindaria A, Grover TA, Aust SD (1994) Oxalate-dependent reductive activity of manganese peroxidase fromPhanerochaete chrysosporium. Arch Biochem Biophys 314:301–306

    CAS  Article  PubMed  Google Scholar 

  19. 19.

    Wariishi H, Valli K, Gold MH (1992) Manganese(II) oxidation by manganese peroxidase from basidiomycetePhanerochaete chrysosporium, kinetic mechanism and role of chelators. J Biol Chem 267:23688–23695

    CAS  PubMed  Google Scholar 

  20. 20.

    Hyde SM, Wood PM (1997) A mechanism for production of hydroxyl radicals by the brown-rot fungusConiophora puteana: Fe(III) reduction by cellobiose dehydrogenase and Fe(II) oxidation at a distance from the hyphae. Microbiology 143:259–266

    CAS  Article  Google Scholar 

  21. 21.

    Blanchette RA (1984) Manganese accumulation in wood decayed by white-rot fungi. Phytopathology 74:725–730

    CAS  Article  Google Scholar 

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Correspondence to Hirofumi Hirai.

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Hirai, H., Kondo, R., Sakai, K. et al. Reduction of ferric chelate caused by various wood-rot fungi. J Wood Sci 45, 262–265 (1999).

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Key words

  • Wood-rot fungus
  • Ferric chelate reduction Manganese peroxidase
  • Gel permeation chromatography