Skip to main content


Official Journal of the Japan Wood Research Society

Journal of Wood Science Cover Image
We’d like to understand how you use our websites in order to improve them. Register your interest.

Reduction of ferric chelate caused by various wood-rot fungi


The reduction of ferric chelate caused by various wood-rot fungi was analyzed. Ferric chelate reductive activity was detected in cell-free extracts of seven wood-rot fungi:Phanerochaete chrysosporium, P. sordida YK-624,Ganoderma sp. YK-505,Coriolus versicolor, Bjerkandera adusta, Tyromyces palustris, andGloeophyllum trabeum. These fungi produced NADPH- or NADH-dependent ferric chelate reductive enzymes (or both) of different molecular weight. In the liquid culture ofP. sordida YK-624 andC. versicolor, a positive correlation was observed between extracellular MnP activity and intracellular NADPH-dependent ferric chelate reductive activity.


  1. 1.

    Raymond KN, Muller G, Matzanke BF (1984) Complexation of iron by siderophores: a review of their solution and structural chemistry and biological function. Top Curr Chem 123:49–1023

  2. 2.

    Wood PM (1988) The potential diagram for oxygen at pH 7. Biochem J 253:287–289

  3. 3.

    Highley TL (1977) Requirements for cellulose degradation by a brown-rot fungus. Mater Org 12:25–36

  4. 4.

    Kirk TK, Ibach R, Mozuch MD, Conner AH, Highley TL (1991) Characteristics of cotton cellulose depolymerized by a brownrotting fungus, by acid, or by chemical oxidants. Holzforschung 45:239–244

  5. 5.

    Hirai H, Kondo R, Sakai K (1997) A model system for NAD(P)Hdependent reduction of manganese dioxide mediated by ferrous chelate in white-rot fungusPhanerochaete sordida YK-624. Mokuzai Gakkaishi 43:247–253

  6. 6.

    Page WJ, Huyer M (1984) Derepression of the Azotobacter vinelandii siderophore system, using iron-containing minerals to limit iron repletion. J Bacteriol 158:496–502

  7. 7.

    Lesuisse E, Raguzzi F, Crichton R (1987) Iron uptake by the yeastSaccharomyces cerevisiae: involvement of a reduction step. J Gen Microbiol 133:3228–3236

  8. 8.

    Georgatsou E, Alexandraki D (1994) Two distinctly regulated genes are required for theSaccharomyces cerevisiae. Mol Cell Biol 14:3065–3073

  9. 9.

    Bao W, Renganathan V (1992) Cellobiose oxidase ofPhanerochaete chrysosporium enhances crystalline cellulose degradation by cellulases. FEBS Lett 302:77–80

  10. 10.

    Bao W, Usha SN, Renganathan V (1993) Purification and characterization of cellobiose dehydrogenase, a novel extracellular hemoflavoenzyme from the white-rot fungusPhanerochaete chrysosporium. Arch Biochem Biophys 300:705–713

  11. 11.

    Henriksson G, Pettersson G, Johansson G, Ruiz A, Uzcategui E (1991) Cellobiose oxidase from Phanerochaete chrysosporium can be cleaved by papain into two domains. Eur J Biochem 196:101–106

  12. 12.

    Kremer SM, Wood PM (1992) Evidence that cellobiose oxidase fromPhanerochaete chrysosporium is primarily an Fe(III) reductase: kinetic comparison with neutrophil NADPH oxidase and yeast flavocytochrome b2. Eur J Biochem 205:133–138

  13. 13.

    Brock B, Rieble S, Gold MH (1995) Purification and characterization of a 1,4-benzoquinone reductase from the basidiomycetePhanerochaete chrysosporium. Appl Environ Microbiol 61:3076–3081

  14. 14.

    Brock BJ, Gold MH (1996) 1,4-Benzoquinone reductase from the basidiomycete Phanerochaete chrysosporium: spectral and kinetic analysis. Arch Biochem Biophys 331:31–40

  15. 15.

    Stahl JD, Aust SD (1995) Properties of a transplasma membrane redox system ofPhanerochaete chrysosporium. Arch Biochem Biophys 320:369–374

  16. 16.

    Hirai H, Kondo R, Sakai K (1998) NADPH-dependent ferrireductase produced by white-rot fungusPhanerochaete sordida YK-624. J Wood Sci 44:369–374

  17. 17.

    Hirai H, Kondo R, Sakai K (1994) Screening of lignin-degrading fungi and their ligninolytic enzyme activities during biological bleaching of kraft pulp. Mokuzai Gakkaishi 40:980–986

  18. 18.

    Khindaria A, Grover TA, Aust SD (1994) Oxalate-dependent reductive activity of manganese peroxidase fromPhanerochaete chrysosporium. Arch Biochem Biophys 314:301–306

  19. 19.

    Wariishi H, Valli K, Gold MH (1992) Manganese(II) oxidation by manganese peroxidase from basidiomycetePhanerochaete chrysosporium, kinetic mechanism and role of chelators. J Biol Chem 267:23688–23695

  20. 20.

    Hyde SM, Wood PM (1997) A mechanism for production of hydroxyl radicals by the brown-rot fungusConiophora puteana: Fe(III) reduction by cellobiose dehydrogenase and Fe(II) oxidation at a distance from the hyphae. Microbiology 143:259–266

  21. 21.

    Blanchette RA (1984) Manganese accumulation in wood decayed by white-rot fungi. Phytopathology 74:725–730

Download references

Author information



Corresponding author

Correspondence to Hirofumi Hirai.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Hirai, H., Kondo, R., Sakai, K. et al. Reduction of ferric chelate caused by various wood-rot fungi. J Wood Sci 45, 262–265 (1999).

Download citation

Key words

  • Wood-rot fungus
  • Ferric chelate reduction Manganese peroxidase
  • Gel permeation chromatography