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Official Journal of the Japan Wood Research Society

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Isolation and structural characterization of rhamnogalacturonan II-borate complex fromPinus densiflora

Journal of Wood Science volume 45, pages 435–439 (1999)Cite this article

Abstract

A boron-containing pectic polysaccharide was isolated from Driselase digests of akamatsu (Pinus densiflora) cell wall. The boron-containing polysaccharide was purified by successive column chromatography of Cosmosil C18 OPN, DEAE Sepharose FF, and Superdex 75. The complex had 0.12% (w/w) boron (B) and11B-NMR spectroscopy showed the complex to be the tetrahedral diester form of boron. The sugar composition and glycosyl linkage analyses showed that the sugar portion of the complex was rhamnogalacturonan-II (RG-11). The boron-attached glycosyl residue was identified to be 2-O-Me-xylose-containing apiosyl side chain in RG-II. These results established that the location of borate in the cell wall is conserved among dicots, gramineous monocots, and coniferous gymnosperm.

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Authors and Affiliations

  1. Forest and Forest Products Research Institute, PO Box 16, 305-8687, Tsukuba Norm, Kenkyu Danchinai, Ibaraki, Japan

    Tomoko Shimokawa & Tadashi Ishii

  2. Kyushu National Agricultural Experiment Station, 861-1192, Nichigoshi, Kumamoto, Japan

    Toshiro Matsunaga

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  1. Tomoko Shimokawa
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  2. Tadashi Ishii
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  3. Toshiro Matsunaga
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Correspondence to Tadashi Ishii.

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Shimokawa, T., Ishii, T. & Matsunaga, T. Isolation and structural characterization of rhamnogalacturonan II-borate complex fromPinus densiflora . J Wood Sci 45, 435–439 (1999). https://doi.org/10.1007/BF01177918

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