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Determination of the distribution and reaction of polysaccharides in wood cell walls by the isotope tracer technique VIII: Selective radiolabeling of xylan in mature cell walls of magnolia (Magnolia kobus DC.) and visualization of its distribution by microautoradiography

Abstract

To radiolabel xylan in mature cell walls selectively, magnolia (Magnolia kobus DC.) was administered withmyo-inositol-[2-3H] and allowed to metabolize for 1 month. The radiolabeled xylem tissue was then submitted to sulfuric acid hydrolysis and nitrobenzene oxidation. A large amount of radioactivity was found mainly in xylose, although slight activities were detected in glucose and in vanillin and syringaldehyde. The labeled tissue was submitted to a preparation of holocellulose followed by treatment with 24% potassium hydroxide (KOH). Radioactivity was distributed mainly in the KOH-soluble part of the holocellulose. These results indicate that most radioactivity was incorporated into xylan in the cell walls. The distribution of the incorporated radioactivity in the xylem tissue was visualized by microautoradiography. Radioactivities were distributed in the xylem more than 400 μm from the cambium; and an inner layer of a secondary wall had formed at the labeled xylem. Consequently, selective radio-labeling of xylan was visualized in mature cell walls.

References

  1. 1.

    Fukushima K, Terashima N (1991) Heterogeneity in formation of lignin. Part XV. Formation and structure of lignin in compression wood ofPinus thunbergii studied by microautoradiography. Wood Sci Technol 25:371–381

  2. 2.

    He LF, Terashima N (1990) Formation and structure of lignin in monocotyledons. 111. Heterogeneity of sugarcane (Saccharum offzcinarum L.) lignin with respect to the composition of structural units in different morphological regions. J Wood Chem Technol 10:435–459

  3. 3.

    Imai T, Terashima N (1992) Determination of the distribution and reaction of polysaccharides in wood cell walls by the isotope tracer technique. III. Visualization of the deposition and distribution of galacturonan in the cell wall of magnolia (Magnolia kobus DC.) xylem by microautoradiography. Mokuzai Gakkaishi 38:475–481

  4. 4.

    Imai T, Terashima N (1992) Determination of the distribution and reaction of polysaccharides in wood cell walls by the isotope tracer technique. IV. Selective radio-labeling of xylan in magnolia (Magnolia kobus DC.) and visualization of its distribution in differentiating xylem by microautoradiography. Mokuzai Gakkaishi 38:693–699

  5. 5.

    Imai T, Yasuda S, Terashima N (1997) Determination of the distribution and reaction of polysaccharides in wood cell walls by the isotope tracer technique. V. Behavior of xylan during kraft pulping studied by radiotracer technique. Mokuzai Gakkaishi 43:241–246

  6. 6.

    Imai T, Terashima N, Yasuda S (1998) Determination of the distribution and reaction of polysaccharides in wood cell walls by the isotope tracer technique. VII. Double radiolabeling of xylan and pectin in magnolia (Magnolia kobus DC.) and comparison of their behaviors during kraft pulping by radiotracer technique. J Wood Sci 44:106–110

  7. 7.

    Imai T, Terashima N (1990) Determination of the distribution and reaction of polysaccharides in wood cell walls by the isotope tracer technique. I. Selective radio-labeling of polysaccharides inMagnolia kobus. Mokuzai Gakkaishi 36:917–922

  8. 8.

    Imai T, Terashima N (1991) Determination of the distribution and reaction of polysaccharides in wood cell walls by the isotope tracer technique. II. Selective radio-labeling of pectic substances in mitsumata (Edgeworthia papyrifera). Mokuzai Gakkaishi 37:733–740

  9. 9.

    Imai T, Terashima N, Yasuda S (1997) Determination of the distribution and reaction of polysaccharides in wood cell walls by the isotope tracer technique. VI. Selective radio-labeling of mannan inGinkgo biloba. Mokuzai Gakkaishi 43:342–348

  10. 10.

    Terashima N, Fukushima K, Tsuchiya S, Takabe K (1986) Heterogeneity in formation of lignin. VII. An autoradiographic study on the formation of guaiacyl and syringyl lignin in poplar. J Wood Chem Technol 6:495–504

  11. 11.

    He LF, Terashima N (1989) Formation and structure of lignin in monocotyledon. I. Selective labeling of the structural units of lignin in rice plant (Oryza sativa) with3H and visualization of their distribution in the tissue by micro autoradiography. Mokuzai Gakkaishi 35:116–122

  12. 12.

    Westermark U, Hardell HL, Iversen T (1986) The content of protein and pectin in the lignified middle lamella/primary wall from spruce fibers. Holzforschung 40:65–68

  13. 13.

    Takeuchi Y, Komamine A (1980) Turnover of cell wall polysaccharides of aVinca rosea suspension culture. I. Synthesis and degradation of cell wall components. Physiol Plantarum 48:271–277

  14. 14.

    Lorences EP, Acebes JL, Revilla G (1989) Change in pectic and hemicellulosic polysaccharides during acid pH-induced growth in pine hypocotyl segments. Plant Sci 62:53–61

  15. 15.

    Gibeaut DM, Carpita NC (1991) Tracing cell wall biogenesis in intact cells and plant: selective turnover and alteration of soluble and cell wall polysaccharides in grasses. Plant Physiol 97:551–561

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Correspondence to Takanori Imai.

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Imai, T., Goto, H., Matsumura, H. et al. Determination of the distribution and reaction of polysaccharides in wood cell walls by the isotope tracer technique VIII: Selective radiolabeling of xylan in mature cell walls of magnolia (Magnolia kobus DC.) and visualization of its distribution by microautoradiography. J Wood Sci 45, 164–169 (1999). https://doi.org/10.1007/BF01192335

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Key words

  • Xylan
  • Selective radiolabeling
  • Microautoradiography
  • Myo-inositol
  • Magnolia kobus DC