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Journal of Wood Science

Official Journal of the Japan Wood Research Society

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Biological activity of extracts fromCupressus lusitanica cell culture

Journal of Wood Science volume 45, pages 170–173 (1999)Cite this article

Abstract

Ethyl acetate extracts ofCupressus lusitanica suspension cell cultures were examined for their biological activities (viz., tyrosinase inhibitory, antioxidant, and antimicrobial activities). The extract from elicitor-treated cells showed all of the biological activities, whereas the extract from cultures without elicitor was not bioactive to a discernible extent. The biological activities shown by the cell culture extracts were almost solely accounted for byβ-thuj aplicin contained in the extracts. These results suggest that the ethyl acetate extract ofC. lusitanica cells treated with the elicitor is a valuable bioactive source without isolation or purification ofβ-thujaplicin.

References

  1. Tabata M (1996) The mechanism of shikonin biosynthesis inLithospermum cell cultures. Plant Tissue Culture Lett 13:117–125

    Article  CAS  Google Scholar 

  2. Yamamoto Y, Kinoshita Y, Takahashi A (1996) Anthocyanin production of culturedEuphorbia millii cells. Plant Tissue Culture Lett 13:249–257

    Article  CAS  Google Scholar 

  3. Yamada Y (1997) Plant biotechnology of tropane alkaloids. Plant Biotechnol 14:1–10

    Article  CAS  Google Scholar 

  4. Mirjalili N, Linden JC (1996) Methyl jasmonate induced production of taxol in suspension cultures ofTaxus cuspidata: ethylene interaction and induction models. Biotechnol Prog 12:110–118

    Article  CAS  Google Scholar 

  5. Tachibana S, Watanabe E, Itoh K, Oki T (1994) Formation of taxol inTaxus cuspidata Sieb. et Zucc. var.nara Rehder callus culture. Mokuzai Gakkaishi 40:1254–1258

    CAS  Google Scholar 

  6. Saito K, Okabe T, Fukui T, Inamori Y (1997) Antibacterial activity of hinokitiol against methicillin-resistantStaphylococcus aureus (MRSA). II. Applications to the disinfections of hospital environments (in Japanese). Mokuzai Gakkaishi 43:882–891

    CAS  Google Scholar 

  7. Okabe T, Saito K, Otomo Y (1989) Antimicrobial activity and safety of hinokitiol (in Japanese). Fragrance J 17:74–79

    CAS  Google Scholar 

  8. Inamori Y, Nishiguti K, Matsuo N, Tsujibo H, Baba K, Ishida N (1991) Phytogrowth-inhibitory activities of tropolone and hinokitiol. Chem Pharm Bull 39:2378–2381

    Article  CAS  Google Scholar 

  9. Inamori Y, Tsujibo H, Ohishi H, Ishii F, Mizugaki M, Aso H, Ishida N (1993) Cytotoxic effect of hinokitiol and tropolone on the growth of mammalian cells and on blastogenesis of mouse splenic T cells. Biol Pharm Bull 16:521–523

    Article  CAS  Google Scholar 

  10. Arima Y, Hatanaka A, Tsukihara S, Fujimoto K, Fukuda K, Sakurai H (1997) Acavenging activities ofα-, β-, γ-thujaplicins against active oxygen species. Chem Pharm Bull 45:1881–1886.

    Article  CAS  Google Scholar 

  11. Belleau B, Burba J (1963) Occupancy of adrenergic receptors and inhibition of catechol O-methyl transferase by tropolones. J Med Chem 6:755–763

    Article  CAS  Google Scholar 

  12. Bohme G, Schonfeld P, Kuster U, Kunz W, Lyr H (1980) The multifunctional actions ofβ-thujaplicin on the oxidative energy transformations as a consequence of its lipophilic and chelating properties. Acta Biol Med Germ 39:1153–1163

    CAS  PubMed  Google Scholar 

  13. Inada S, Tsutsumi Y, Sakai K (1993) Elicitor of theβ-thujaplicin accumulation in callus cultures ofCupressus lusitanica. J Fac Agr Kyushu Univ 38:119–126

    CAS  Google Scholar 

  14. Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50:151–158

    Article  CAS  Google Scholar 

  15. tose R, Sakai K (1997) Improved culture conditions for the production ofβ-thujaplicin by suspension cell cultures ofCupressus lusitanica. Plant Biotechnol 14:163–167

    Article  Google Scholar 

  16. Sakai K, Yamaguchi T, Itose R (1997) Biosynthesis of a heartwood tropolone,β-thujaplicin. Mokuzai Gakkaishi 43:696–698

    CAS  Google Scholar 

  17. Morita H, Kayashita T, Kobata H, Gonda A, Takeya K, Itokawa H (1994) Pseudostellarins D-F, new tyrosinase inhibitory cyclic peptides fromPseudostellaria heterophylla. Tetrahedron 50:9975–9982

    Article  CAS  Google Scholar 

  18. Osawa T, Ide A, Su JD, Namiki M (1987) Inhibition of lipid peroxidation by ellagic acid. J Agric Food Chem 35:808–812

    Article  CAS  Google Scholar 

  19. Koch RB, Stern B, Ferrari CG (1958) Linoleic acid and trilinolein as substrates for soybean lipoxidase(s). Arch Biochem Biophys 78:165–179

    Article  CAS  Google Scholar 

  20. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Forest Products, Faculty of Agriculture, Kyushu University, 812-8581, Fukuoka, Japan

    Takashi Yamaguchi, Koki Fujita & Kokki Sakai

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  1. Takashi Yamaguchi
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  2. Koki Fujita
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  3. Kokki Sakai
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Correspondence to Kokki Sakai.

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Yamaguchi, T., Fujita, K. & Sakai, K. Biological activity of extracts fromCupressus lusitanica cell culture. J Wood Sci 45, 170–173 (1999). https://doi.org/10.1007/BF01192336

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