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Effect of coniferyl alcohol addition on removal of chlorophenols from water effluent by fungal laccase


The effect of coniferyl alcohol on removal of chlorinated phenols from a water environment byRhizoctonia praticola andCerrena unicolor laccases was studied. At optimal conditions in which 7 mM coniferyl alcohol and laccase were added to chlorinated phenols over 20h, about 34% of the radioactivity of 4-chlorophenol, 57% of 2,4-dichlorophenol, 66% of 2,4,5-trichlorophenol, and 85% of pentachlorophenol were removed from the supernatants, compared to the level without laccase activity. After 12-h incubation periods at the optimal concentrations of coniferyl alcohol and laccase (added simultaneously), the fast first phase of chlorophenol removal was complete in 1 h, and eventually coniferyl alcohol enhanced the removal of 4-chlorophenol by 40%, 2,4-dichlorophenol by 54%, 2,4,5-trichlorophenol by 60%, and pentachlorophenol by 76%.


  1. 1.

    Bollag JM, Leonowicz A (1984) Comparative studies of extracellular fungal laccases. Appl Env Microbiol 48:849–854

    CAS  Google Scholar 

  2. 2.

    Bourbonnais R, Paice MG, Reid ID (1992) Lignin oxidation and pulp delignification by laccase ofTrametes versicolor in the presence of ABTS. In: Kuwahara M, Shimada M (eds) Biotechnology in pulp and paper industry. Uni Publishers, Tokyo, pp 181–186

    Google Scholar 

  3. 3.

    Bourbonnais R, Paice MG, Reid ID, Lanthier P, Yaguchi M (1995) Lignin oxidation by laccase isozyme fromTrametes versicolor and role of the mediator 2,2′-azinobis(3-ethylbenzthiazoline-6-sulfonate) in kraft lignin depolymerization. Appl Env Microbiol 61:1876–1880

    CAS  Google Scholar 

  4. 4.

    Manzanares P, Fajardo S, Martin C (1995) Production of ligninolytic activities when treating paper pulp effluents byTrametes versicolor. J Biotechnol 43:15–132

    Article  Google Scholar 

  5. 5.

    Roy-Arcand L, Archibald FS (1991) Direct dechlorination of chlorophenolic compounds by laccases fromTrametes versicolor. Enzyme Microb Technol 13:194–203

    CAS  Article  Google Scholar 

  6. 6.

    Altlow SC, Bonadonna AL, Klibanov AM (1984) Dephenolization of industrial waste water catalyzed by polyphenol oxidase. Biotechnol Bioeng 26:599–603

    Article  Google Scholar 

  7. 7.

    Bollag JM (1983) Cross-coupling of humus constituents and xenobiotic substances. In: Christman RF, Gjessing ET (eds)Aquatic and terrestrial humic materials. Ann Arbor Science Publications, Ann Arbor, MI, pp 127–141

    Google Scholar 

  8. 8.

    Nannipieri P, Bollag JM (1991) Use of enzymes to detoxify pesticide-contaminated soils and waters. J Environ Quality 20:510–517

    CAS  Article  Google Scholar 

  9. 9.

    Simmons KE, Minard RD, Bollag JM (1989) Oxidative cooligomerization of guaiacol and 4-chloroaniline. Envron Sci Technol 23:115–121

    CAS  Article  Google Scholar 

  10. 10.

    Klibanov AM, Tu T-M, Scott KP (1983) Peroxidase catalyzed removal of phenols from coal-conversion wastewaters. Science 221:259–261

    CAS  Article  Google Scholar 

  11. 11.

    Berry DF, Boyd SA (1984) Oxidative coupling of phenols and amines by peroxidase: structure-activity relationship. Soil Sci Soc Am J 48:565–569

    CAS  Article  Google Scholar 

  12. 12.

    Roper JC, Sarkar JD, Dec J, Bollag JM (1995) Enhanced enzymatic removal of chlorophenols in the presence of co-substrates. Water Res 29:2720–2724

    CAS  Article  Google Scholar 

  13. 13.

    Sterjiades R, Ranocha P, Boudet AM, Goffner D (1996) Identification of specific laccase isoforms capable of polymerizing monolignols by an “in-gel” procedure. Anal Biochem 242:158–161

    CAS  Article  Google Scholar 

  14. 14.

    Leonowicz A, Edgechill RU, Bollag JM (1984) The effect on the transformation of syringic and vanillic acids by the laccases ofRhizoctonia praticola andTrametes versicolor. Arch Microbiol 137:89–96

    CAS  Article  Google Scholar 

  15. 15.

    Leonowicz A, Grzywnowicz K (1981) Quantitative estimation of laccase forms in some white-rot fungi using syringaldazine as a substrate. Enzyme Microb Technol 3:55–58

    CAS  Article  Google Scholar 

  16. 16.

    Kirk TK, Brunow G (1988) Synthetic14C-labeled lignin. Methods Enzymol 161:65–73

    CAS  Article  Google Scholar 

  17. 17.

    Trojanowski J, Leonowicz A (1962) A quantitative determination of Bjbrkman's lignin in a solution by reaction with phloroglucinol. Ann Univ MC-Sklodowska Sect AA 17:121–126

    Google Scholar 

  18. 18.

    Leonowicz, A, Trojanowski J, Nowak G (1972) Ferulic acid as inductor of messenger RNA synthesis related to laccase formation in wood rotting fungusPleurotus ostreatus. Microbios 6:23–28

    CAS  PubMed  Google Scholar 

  19. 19.

    Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275

    CAS  PubMed  Google Scholar 

  20. 20.

    Leonowicz A, Trojanowski J (1975) Induction of laccase by ferulic acid in basidiomycetes. Acta Biochim Pol 22:291–295

    CAS  PubMed  Google Scholar 

  21. 21.

    Worall JJ, Chet I, Huttermann A (1986) Association of rhizomorph formation with laccase activity inArmillariella spp. J Gen Microbiol 132:2527–2533

    Google Scholar 

  22. 22.

    Leonowicz A, Trojanowski J (1975) Induction of a new laccase form in the fungusPleurotus ostreatus by ferulic acid. Microbios 13:167–174

    CAS  Google Scholar 

  23. 23.

    Wahleithner JA, Xu F, Brown KM, Brown SH, Golithly EJ, Halkier T, Kauppinen S, Pederson A, Shneider P (1996) The identification and characterization of four laccases from the plant pathogenic fungusRhizoctonia solani. Curr Genet 29:395–403

    CAS  Article  Google Scholar 

  24. 24.

    Minard RD, Liu SY, Bollag JM (1981) Oligomers and quinones from 2,4-dichlorophenol. J Agric Food Chem 29:250–253

    CAS  Article  Google Scholar 

  25. 25.

    Lyr H (1963) Enzymatische Detoxifikation chlorierter Phenole. Phytopathol Z 47:73–82

    CAS  Article  Google Scholar 

  26. 26.

    Konishi K, Inoue Y (1972) Detoxification mechanism of pentachlorophenol by the laccase ofCoriolus versicolor. Mokuzai Gakkaishi 18:463–469

    CAS  Google Scholar 

  27. 27.

    Dec J, Bollag JM (1994) Dehalogenation of chlorinated phenols during oxidative coupling. Environ Sci Technol 28:484–490

    CAS  Article  Google Scholar 

  28. 28.

    Dec J, Bollag JM (1995) Effect of various factors on dehalogenation of chlorinated phenols and anilines during oxidative coupling. Environ Sci Technol 29:657–663

    CAS  Article  Google Scholar 

  29. 29.

    Maydoun J, Sarkar J (1975) Laccase dePolyporus versicolor dans le sol et la Litere. Soil Biol Biochem 7:31–34

    Article  Google Scholar 

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Correspondence to Nam-Seok Cho.

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Cho, N., Rogalski, J., Jaszek, M. et al. Effect of coniferyl alcohol addition on removal of chlorophenols from water effluent by fungal laccase. J Wood Sci 45, 174–178 (1999).

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Key words

  • Chlorophenols
  • Coniferyl alcohol
  • Laccase
  • Rhizoctonia praticola
  • Cerrena unicolor