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Journal of Wood Science

Official Journal of the Japan Wood Research Society

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Studies on the immunolabeling of heartwood extractives: characterization of anti-agatharesinol antiserum

Journal of Wood Science volume 50, pages 82–86 (2004)Cite this article

Abstract

The reactivity of previously prepared antiserum against agatharesinol, a norlignan, was determined by competitive inhibition–ELISA (enzyme-linked immunosorbent assay) using several natural compounds that are representative of plant extractives as competitive inhibitors. The antiserum strongly reacted with some norlignans (i.e., agatharesinol, hinokiresinol, and metasequirin C), each of which has a common chemical-structural unit. This result suggests that the antiserum recognizes the specific chemical-structural unit composing those norlignans. Moreover, differences in the reactivities of the antiserum with agatharesinol derivatives and synthetic chalcones propose the trans-3-p-hydroxyphenyl-1-phenylpropene structural unit as the major antigenic determinant for the antiserum.

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Authors and Affiliations

  1. Laboratory of Biomass Resources Utilization, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, 464-8601, Japan

    Takeshi Nagasaki, Norihisa Osada, Mika Sato & Takanori Imai

  2. Laboratory of Forest Chemistry, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, 464-8601, Japan

    Seiichi Yasuda

  3. Section of Forest Resources, Department of Bioenvironment, Faculty of Agriculture, Yamagata University, Tsuruoka, 997-8555, Japan

    Koetsu Takahashi

Authors
  1. Takeshi Nagasaki
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  2. Norihisa Osada
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  3. Mika Sato
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  4. Seiichi Yasuda
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  5. Koetsu Takahashi
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  6. Takanori Imai
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Corresponding author

Correspondence to Takanori Imai.

Additional information

Part of this paper was presented at the 45th Lignin Symposium, Matsuyama, October 2000 and the 51th Annual Meeting of the Japan Wood Research Society, Tokyo, April 2001

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Nagasaki, T., Osada, N., Sato, M. et al. Studies on the immunolabeling of heartwood extractives: characterization of anti-agatharesinol antiserum. J Wood Sci 50, 82–86 (2004). https://doi.org/10.1007/s10086-003-0530-1

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  • DOI: https://doi.org/10.1007/s10086-003-0530-1

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