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Journal of Wood Science

Official Journal of the Japan Wood Research Society

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Immunolocalization of an anionic peroxidase in differentiating poplar xylem

Journal of Wood Science volume 51, pages 317–322 (2005)Cite this article

Abstract

Peroxidases are the major candidate enzymes involved in dehydrogenative polymerization of monolignols. Peroxidases have the signal sequence at their N-terminus and this suggests that they are transported to extracellular spaces or developing cell walls. In this study, we focused on an anionic peroxidase isozyme encoded by prxA3a, which seems to be related to lignification. To investigate the localization of peroxidase in differentiating xylem cells of poplar (Populus sieboldii × Populus grandidentata), anti-PRX3 antibody was raised against the anionic peroxidase. Western blotting and peroxidase activity inhibition assay showed specificity of the antibody. Labeling by anti-PRX3 antibody was localized in vessels and fibers during the secondary wall formation and was observed along the plasma membrane beside the microtubules. The labeling was not seen in the cell wall, where localization of peroxidases was expected during lignification. The peroxidase isozyme, which is suggested to be involved in monolignol polymerization, is localized on the plasma membrane and its localization might be regulated by microtubules.

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Authors and Affiliations

  1. Laboratory of Tree Cell Biology, Division of Forest and Biomaterials Science, Graduate School of Agriculture, Kyoto University, Kyoto, 606-8502, Japan

    Miyuki Takeuchi, Keiji Takabe & Minoru Fujita

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  1. Miyuki Takeuchi
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  2. Keiji Takabe
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  3. Minoru Fujita
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Correspondence to Keiji Takabe.

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Takeuchi, M., Takabe, K. & Fujita, M. Immunolocalization of an anionic peroxidase in differentiating poplar xylem. J Wood Sci 51, 317–322 (2005). https://doi.org/10.1007/s10086-004-0662-y

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  • DOI: https://doi.org/10.1007/s10086-004-0662-y

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