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Journal of Wood Science

Official Journal of the Japan Wood Research Society

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Ultraviolet microspectrophotometric investigation of the distribution of lignin in Prunus jamasakura differentiated on a three-dimensional clinostat

Journal of Wood Science volume 51, pages 448–454 (2005)Cite this article

Abstract

To examine the effect of gravity on lignin content and deposition in plant cells, we used ultraviolet (UV) microspectrophotometry and chemical methods to investigate the secondary xylem of Prunus jamasakura grown on a three-dimensional (3D) clinostat, which simulates microgravity. The stem of the 3D-clinostat specimens elongated with bending and the width of their secondary phloem increased. The UV absorbance of the 3D-clinostat specimens at 278 nm was higher than that of the control specimens, which were grown on the ground, in the wood fiber cell corner middle lamella, compound middle lamella, and fiber secondary wall; the UV absorbance in the vessel secondary wall did not differ between the specimens. The lignin content in the stem, including the bark, of the 3D-clinostat specimens, as determined using an acetyl bromide method, was less than that of the control specimens. In the specimens that differentiated on a 3D clinostat, the amount of lignin in the wood fibers increased, while the proportion of the lignified xylem in the stem decreased relative to control values.

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Authors and Affiliations

  1. Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, 464-8601, Japan

    Masato Yoshida, Daiji Fujiwara, Yukiko Tsuji, Kazuhiko Fukushima & Takashi Okuyama

  2. Faculty of Science, Japan Women's University, Tokyo, 112-8681, Japan

    Teruko Nakamura

Authors
  1. Masato Yoshida
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  2. Daiji Fujiwara
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  3. Yukiko Tsuji
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  4. Kazuhiko Fukushima
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  5. Teruko Nakamura
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  6. Takashi Okuyama
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Correspondence to Masato Yoshida.

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Yoshida, M., Fujiwara, D., Tsuji, Y. et al. Ultraviolet microspectrophotometric investigation of the distribution of lignin in Prunus jamasakura differentiated on a three-dimensional clinostat. J Wood Sci 51, 448–454 (2005). https://doi.org/10.1007/s10086-004-0681-8

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  • DOI: https://doi.org/10.1007/s10086-004-0681-8

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