Skip to main content

Official Journal of the Japan Wood Research Society

Journal of Wood Science Cover Image

Antibody-based assay for galacturonan deesterification on wood pulp fibers during bleaching

Abstract

Antibodies recognizing galacturonan were used in immunoassays for industrial unbleached and hydrogen peroxide-bleached chemithermomechanical pulp (CTMP). The assays were based on a colorimetric method using specific monoclonal antibody recognition of acidic and methylated homogalacturonan, respectively, on the pulp fiber surfaces. Alkaline phosphatase-conjugated antibodies were linked to the galacturonan specific antibodies, and an alkaline phosphatase substrate (p-nitrophenylphosphate) was used to develop a yellow reaction product that can be followed spectrophotometrically. Together the newly developed immunoassays were able to describe the deesterification, specifically, of surface-localized galacturonan on CTMP, induced by alkaline hydrogen peroxide bleaching. Unbleached CTMP showed relatively more labeling by methylated galacturonan recognizing antibodies, whereas bleached CTMP was relatively more labeled for acidic galacturonan. The increase in negative charge of the bleached pulp was also shown by polyelectrolyte titration; the negative surface charge was 9.3 µeq g−1 in unbleached CTMP and 21.7 µeq g−1 in bleached pulp.

References

  1. 1

    R Franzén (1986) ArticleTitleGeneral and selective upgrading of mechanical pulps Nord Pulp Pap Res J 3 4–13 Occurrence Handle10.3183/NPPRJ-1986-01-03-p004-013

    Article  Google Scholar 

  2. 2

    DA Powell ER Morris MJ Gidley DA Rees (1982) ArticleTitleConformations and interactions of pectins. II. Influence of residue sequence on chain association in calcium pectate gels J Mol Biol 155 517–531 Occurrence Handle1:CAS:528:DyaL38XktVamsr0%3D Occurrence Handle10.1016/0022-2836(82)90485-5 Occurrence Handle7086902

    CAS  Article  PubMed  Google Scholar 

  3. 3

    J Hafrén U Westermark (2001) ArticleTitleDistribution of acidic and esterified polygalacturonan in sapwood of spruce, birch and aspen Nord Pulp Pap Res J 4 284–290 Occurrence Handle10.3183/NPPRJ-2001-16-04-p284-290

    Article  Google Scholar 

  4. 4

    J Hafrén G Daniel (2003) ArticleTitleDistribution of methyl-esterified galacturonan in chemical and mechanical pulp fibers J Wood Sci 49 361–365 Occurrence Handle10.1007/s10086-002-0488-4

    Article  Google Scholar 

  5. 5

    Y Zhang B Sjögren P Engstrand M Htun (1994) ArticleTitleDetermination of charged groups in mechanical pulp fibres and their influence on pulp properties J Wood Chem Technol 1 83–102 Occurrence Handle10.1080/02773819408003087

    Article  Google Scholar 

  6. 6

    J Hafrén G Daniel (2003) ArticleTitleA bioassay for methylated galacturonan on pulp-fiber surfaces Biotechnol Lett 25 859–862 Occurrence Handle10.1023/A:1024036803997 Occurrence Handle12889794

    Article  PubMed  Google Scholar 

  7. 7

    O Theander EA Westerlund (1986) ArticleTitleStudies on dietary fiber. 3. Improved procedures for analysis of dietary fiber J Agric Food Chem 34 330–336 Occurrence Handle1:CAS:528:DyaL28Xht1ygs7g%3D Occurrence Handle10.1021/jf00068a045

    CAS  Article  Google Scholar 

  8. 8

    TAPPI test methods (1988) Acid-insoluble lignin in wood and pulp. T 222 om-88. TAPPI, Atlanta, GA

  9. 9

    L Wågberg L Ödberg G Glad-Normark (1989) ArticleTitleCharge determination of porous substrates by polyelectrolyte adsorption. Part I. Carboxymethylated bleached cellulosic fibers Nord Pulp Pap Res J 4 71–76 Occurrence Handle10.3183/NPPRJ-1989-04-02-p071-076

    Article  Google Scholar 

  10. 10

    ISO Standard Test Method (2001) Determination of drainability – part 2: Canadian standard freeness method. ISO 5267-2:2001. Standard handbook; paper, board and pulps. International Organization for Standardization, Geneva

  11. 11

    KA van den Bosch DJ Bradley JP Knox S Perotto GW Butcher NJ Brewin (1989) ArticleTitleCommon components of the infection thread matrix and the intercellular space identified by immunocytochemical analysis of pea nodules and uninfected roots EMBO J 8 335–342 Occurrence Handle1:CAS:528:DyaL1MXhslShtbY%3D

    CAS  Google Scholar 

  12. 12

    JP Knox PJ Linstead J King C Cooper K Roberts (1990) ArticleTitlePectin esterification is spatially regulated both within cell walls and between developing tissues of root apices Planta 181 512–521 Occurrence Handle1:CAS:528:DyaK3cXlt12nsbo%3D Occurrence Handle10.1007/BF00193004 Occurrence Handle24196931

    CAS  Article  PubMed  Google Scholar 

  13. 13

    B Holmbom R Ekman R Sjöholm C Eckerman J Thornton (1991) ArticleTitleChemical changes in peroxide bleaching of mechanical pulps Das Papier 45 16–22 Occurrence Handle1:CAS:528:DyaK38XisFOltro%3D

    CAS  Google Scholar 

  14. 14

    WGT Willats PM Gilmartin JD Mikkelsen JP Knox (1999) ArticleTitleCell wall antibodies without immunization: generation and use of de-esterified homogalacturonan block-specific antibodies from a naive phage display library Plant J 18 57–65 Occurrence Handle1:CAS:528:DyaK1MXjtVyqtb4%3D Occurrence Handle10.1046/j.1365-313X.1999.00427.x Occurrence Handle10341443

    CAS  Article  PubMed  Google Scholar 

  15. 15

    WGT Willats G Limberg HC Buchholt G-J van Alebeek J Benen TMIE Christensen J Visser A Vorangen JD Mikkelsen JP Knox (2000) ArticleTitleAnalysis of pectic epitopes recognised by hybridoma and phage display monoclonal antibodies using defined oligosaccharides, polysaccharides, and enzymatic degradation Carbohydr Res 327 309–320 Occurrence Handle1:CAS:528:DC%2BD3cXlt1yqsrk%3D Occurrence Handle10.1016/S0008-6215(00)00039-2 Occurrence Handle10945679

    CAS  Article  PubMed  Google Scholar 

  16. 16

    J Gierer (1997) ArticleTitleFormation and involvement of superoxide (O2 −./HO2 .) and hydroxyl (OH.) radicals in TCF bleaching processes: a review Holzforschung 51 34–46 Occurrence Handle1:CAS:528:DyaK2sXltFGitbw%3D Occurrence Handle10.1515/hfsg.1997.51.1.34

    CAS  Article  Google Scholar 

Download references

Author information

Affiliations

Authors

Corresponding author

Correspondence to Jonas Hafrén.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Hafrén, J. Antibody-based assay for galacturonan deesterification on wood pulp fibers during bleaching. J Wood Sci 51, 655–658 (2005). https://doi.org/10.1007/s10086-005-0718-7

Download citation

Key words

  • Charge
  • Chemithermomechanical pulping
  • Immunoassay
  • Pectin