Skip to main content
Journal of Wood Science

Official Journal of the Japan Wood Research Society

Download PDF
  • Note
  • Published:

Antimicrobial activity of heartwood components of sugi (Cryptomeria japonica) against several fungi and bacteria

Journal of Wood Science volume 52, pages 552–556 (2006)Cite this article

Abstract

Methanol extract of sawdust of sugi (Cryptomeria japonica) heartwood was fractionated with toluene and n-hexane to give solvent-soluble and solvent-insoluble fractions. The n-hexane-soluble fraction showed the most inhibition activity among the fractions against phytopathogenic microorganisms, namely Fusarium oxysporum, Phytophthora capsici, Pythium splendens, and Ralstonia solanacearum. Sandaracopimarinol and ferruginol, isolated from the n-hexane-soluble fraction, showed moderate antifungal activity against the three fungi and strong antibacterial activity against R. solanacearum. The content of sandaracopimarinol (7.07 g/kg based on the dried sawdust) in the heartwood was about twice that of ferruginol. Sandaracopimarinol and ferruginol strongly inhibited the growth of Gram-positive bacteria but did not show inhibitory action against Gram-negative bacteria except for R. solanacearum. The antibacterial effect of sandaracopimarinol was first found in the present study and was stronger than that of ferruginol.

References

  1. Morita S, Yatagai M, Ohira T (1991) Antimite and antifungal activities of the hexane extractives from Yakusugi bogwood (in Japanese). Mokuzai Gakkaishi 37:352–357

    CAS  Google Scholar 

  2. Morita S, Hidaka T, Yatagai M (1997) Antifungal components of the extractives of Yakusugi (Cryptomeria japonica D. Don) (in Japanese). Mokuzai Hozon 23:61–69

    Article  Google Scholar 

  3. Nakajima K, Yoshimoto T, Fukuzumi T (1980) Substances inhibiting growth of shiitake mycelium in sugi wood (Cryptomeria japonica D. Don) (in Japanese). Mokuzai Gakkaishi 26:698–702

    CAS  Google Scholar 

  4. Kawachi S, Meguro S, Inada S (1991) Cultivation of shiitake (Lentinus edodes) on wood-meal medium of Cryptomeria japonica. Inhibitory effect of ferruginol on mycelial growth (in Japanese). Mokuzai Gakkaishi 37:971–975

    CAS  Google Scholar 

  5. Matsui T, Matsushita Y, Sugamoto K, Ogawa K, Kimiyama A, Muta S (2001) Mycelial growth inhibition of shiitake (Lentinula edodes) by several terpenoids isolated from sugi (Cryptomeria japonica) wood (in Japanese). Mokuzai Gakkaishi 47:58–62

    CAS  Google Scholar 

  6. Kofujita H, Fujino Y, Sasaki T, Hasebe M, Ota M, Suzuki K (2001) Antifungal activity of the bark of Cryptomeria japonica and its relevant components (in Japanese). Mokuzai Gakkaishi 47:479–486

    CAS  Google Scholar 

  7. Kofujita H, Ota M, Takahashi K, Kawai Y, Hayashi Y (2002) A diterpene quinone from the bark of Cryptomeria japonica. Phytochemistry 61:895–898

    Article  CAS  PubMed  Google Scholar 

  8. Arihara S, Umeyama A, Bando S, Imoto S, Ono M, Yoshikawa K (2004) Three new sesquiterpenes from the black heart wood of Cryptomeria japonica. Chem Pharm Bull 52:463–465

    Article  CAS  PubMed  Google Scholar 

  9. Cheng SS, Lin HY, Chang ST (2005) Chemical composition and antifungal activity of essential oils from different tissues of Japanese cedar (Cryptomeria japonica). J Agric Food Chem 53:614–619

    Article  CAS  PubMed  Google Scholar 

  10. Lee CK, Chang MH (2000) The chemical constituents from the heartwood of Eucalyptus citriodora. J Chinese Chem Soc 47:555–560

    Article  CAS  Google Scholar 

  11. Matsui T, Yoshida K, Nakayama N, Mizutani M (1991) Isolation and chemical structures of constituents of steam exploded Sugi (Cryptomeria japonica D. Don) wood (in Japanese). Bull Fac Eng Miyazaki Univ 37:33–39

    Google Scholar 

  12. Matsumoto T, Ohsuga Y, Harada S, Fukui K (1977) Synthesis of taxodione, royleanone, cryptojaponol, and methyl 11-hydroxy-12-methoxy-7-oxoabieta-8,11,13-trien-18-oate. Bull Chem Soc Jpn 50:266–272

    Article  CAS  Google Scholar 

  13. Sogabe A, Kinjo K, Abe F, Yamauchi T, Yaga S (2000) Termiticidal substances from the heartwood of Cryptomeria japonica (in Japanese). Mokuzai Gakkaishi 46:124–131

    CAS  Google Scholar 

  14. Aoyama M, Mori M, Okumura M, Doi S, Anetai M (1997) Antifungal activity of 2′,6′-dihydroxy-4′-methoxyacetophenone and related compounds. Mokuzai Gakkaishi 43:108–111

    CAS  Google Scholar 

  15. The Japanese Society of Chemotherapy (1981) Method for in vitro determination of bacterial susceptibility (minimum inhibitory concentration; MIC) to antibacterial agents (in Japanese). Chemotherapy 29:76–79

    Google Scholar 

  16. Kubo I, Muroi H, Himejima M (1992) Antibacterial activity of totarol and its potentiation. J Nat Prod 55:1436–1440

    Article  CAS  PubMed  Google Scholar 

  17. Haraguchi H, Oike S, Muroi H, Kubo I (1995) Mode of antibacterial action of totarol, a diterpene from Podocarpus nagi. Planta Medica 62:122–125

    Article  Google Scholar 

  18. Nicolson K, Evans G, O’Toole PW (1999) Potentiation of methicillin activity against methicillin-resistant Staphylococcus aureus by diterpenes. FEMS Microbiol Lett 179:233–239.

    Article  CAS  PubMed  Google Scholar 

  19. Ashitani T, Ujike M, Nagahama S, Ueno T, Sakai K (2001) Characterization of sugi (Cryptomeria japonica) bark extracts (in Japanese). Mokuzai Gakkaishi 47:276–281

    CAS  Google Scholar 

  20. Kobayashi K, Nishino C, Fukushima M, Shiobara Y, Kodama M (1988) Antibacterial activity of pisiferic acid and its derivatives against Gram-negative and-positive bacteria. Agric Biol Chem 52:77–83

    CAS  Google Scholar 

  21. Muhammad I, Mossa JS, El-Feraly FS (1996) Additional antibacterial diterpenes from the bark of Juniperus procera. Phytother Res 10:604–607

    Article  CAS  Google Scholar 

  22. Evans GB, Furneaux RH, Gravestock MB, Lynch GP, Scott GK (1999) The synthesis and antibacterial activity of totarol derivatives. Part 1: modifications of ring-C and pro-drugs. Bioorg Med Chem 7:1953–1964

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Faculty of Engineering, University of Miyazaki, Gakuen-Kibanadai-Nishi, Miyazaki, 889-2192, Japan

    Yoh-ichi Matsushita, Young-Hee Hwang, Kazuhiro Sugamoto & Takanao Matsui

Authors
  1. Yoh-ichi Matsushita
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Young-Hee Hwang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Kazuhiro Sugamoto
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Takanao Matsui
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yoh-ichi Matsushita.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Matsushita, Yi., Hwang, YH., Sugamoto, K. et al. Antimicrobial activity of heartwood components of sugi (Cryptomeria japonica) against several fungi and bacteria. J Wood Sci 52, 552–556 (2006). https://doi.org/10.1007/s10086-005-0793-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10086-005-0793-9

Key words