Skip to main content
Journal of Wood Science

Official Journal of the Japan Wood Research Society

Download PDF
  • Note
  • Published:

Proteins in Norway spruce thermomechanical pulp

Journal of Wood Science volume 53, pages 143–146 (2007)Cite this article

Abstract

Two methods of cytochemical staining using Coomassie dye and Cu+-bicinchoninic acid, respectively, showed that there are proteins in thermomechanical pulp (TMP) of Norway spruce. Protein isolated from TMP was analyzed for amino acid composition. There was about twice the amount of acidic amino acid material compared with basic amino acids, and the presence of glucosamine indicated that the isolated polypeptides also contained glycoproteins. The presence of proteins in ray cells and fiber tracheids in TMP adds to the chemical heterogeneity of the structurally complex high-yield pulp.

References

  1. Simson BW, Timell TE (1978) Polysaccharides in cambial tissues of Populus tremuloides and Tilia americana. I. Isolation, fractionation, and chemical composition of the cambial tissues. Cell Chem Technol 12:39–50

    CAS  Google Scholar 

  2. Mellerowicz E, Baucher M, Sundberg B, Boerjan W (2001) Unraveling cell wall formation in the woody dicot stem. Plant Mol Biol 47:239–274

    Article  CAS  PubMed  Google Scholar 

  3. Cassab GI (1998) Plant cell wall proteins. Ann Rev Plant Physiol Plant Mol Biol 49:281–309

    Article  CAS  Google Scholar 

  4. Westermark U, Hardell H-L, Iversen T (1986) The content of protein and pectin in the lignified middle lamella/primary wall from spruce fibers. Holzforschung 40:65–68

    Article  CAS  Google Scholar 

  5. Smith PK, Krohn RI, Hermanson GT, Mallia AK, Gartner FH, Provenzano MD, Fujimoto EK, Goeke NM, Olson Klenk DC (1985) Measurement of protein using bichinonic acid. Anal Biochem 150:76–85

    Article  CAS  PubMed  Google Scholar 

  6. Fry SC (1988) The growing plant cell wall: chemical and metabolic analysis. Longman, London, p 72

    Google Scholar 

  7. Hausalo T (1985) Analysis of wood and pulp carbohydrates by anion exchange chromatography with pulsed amperometric detection. Proceedings of the 8th International Symposium on Wood Pulp Chemistry, Helsinki, pp 131-136

  8. TAPPI Test Methods (1988) Acid-insoluble lignin in wood and pulp. T 222 om-88. TAPPI, Atlanta, GA

    Google Scholar 

  9. International Organization for Standardization (ISO) (2001) Determination of drainability part 2: “Canadian standard” freeness method. ISO 5267-2:2001. Standard handbook; paper, board and pulps. International Organization for Standardization, Geneva

    Google Scholar 

  10. Hafrén J, Westermark U, Lennholm L, Terashima N (2002) Formation of 13C-enriched cell wall DHP using isolated soft xylem from Picea abies. Holzforschung 56:585–591

    Article  Google Scholar 

  11. Brumer H, Zhou Q, Baumann MJ, Carlsson K, Teeri TT (2004) Activation of crystalline cellulose surfaces through the chemoenzymatic modification of xyloglucan. J Am Chem Soc 126:5715–5721

    Article  CAS  PubMed  Google Scholar 

  12. Franzén R (1986) General and selective upgrading of mechanical pulps. Nordic Pulp Pap Res J 3:4–13

    Article  Google Scholar 

  13. Scurfield G, Nicholls PW (1970) Amino acid composition of wood proteins. J Exp Bot 21:857–868

    Article  CAS  Google Scholar 

  14. Powell DA, Morris ER, Gidley MJ, Rees DA (1982) Conformation and interactions of pectins. II. Influence of residue sequence on chain association in calcium pectate gels. J Mol Biol 155:517–531

    Article  CAS  PubMed  Google Scholar 

  15. Hafrén J, Daniel G, Westermark U (2000) The distribution of acidic and esterified pectin in cambium, developing xylem and mature xylem of Pinus sylvestris. IAWA J 21:157–168

    Article  Google Scholar 

  16. Hafrén J, Westermark U (2001) Distribution of acidic and esterified polygalacturonan in sapwood of spruce, birch and aspen. Nordic Pulp Pap Res J 4:284–290

    Article  Google Scholar 

  17. Peng FH, Westermark U (1997) Distribution of coniferyl alcohol and coniferaldehyde groups in the cell wall of spruce fibres. Holzforschung 51:531–536

    Article  CAS  Google Scholar 

  18. Hafrén J, Daniel G (2003) Distribution of methylesterified galacturonan in chemical and mechanical pulp fibers. J Wood Sci 49:361–365

    Article  Google Scholar 

  19. Hafrén J, Daniel G (2003) A bioassay for methylated galacturonan on pulp-fiber surfaces. Biotechnol Lett 25:859–862

    Article  PubMed  Google Scholar 

  20. Börås L, Gatenholm P (1999) Surface composition and morphology of CTMP fibers. Holzforschung 53:188–194

    Google Scholar 

  21. Koljonen K, Österberg M, Stenius P (2001) Surface chemistry and morphology of mechanical pulp fibres. Proceedings of the international mechanical pulping conference, Helsinki, pp 305-314

  22. Kleen M, Kangas H, Laine C (2003) Chemical characterization of mechanical pulp fines and fiber surface layers. Nordic Pulp Pap Res J 4:361–368

    Article  Google Scholar 

  23. Mansfield SD, Wong KK, Richardson JD (1999) Improvements in mechanical pulp processing with proteinase treatments. Proceedings of 53rd Appita Annual Conference, Rotorua, New Zealand, pp 375-381

Download references

Author information

Authors and Affiliations

  1. Department of Wood Science, Swedish University of Agricultural Sciences, Wood Ultrastructure Research Centre (WURC), Box 7008, Uppsala, SE-750 07, Sweden

    Jonas Hafrén

Authors
  1. Jonas Hafrén
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jonas Hafrén.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hafrén, J. Proteins in Norway spruce thermomechanical pulp. J Wood Sci 53, 143–146 (2007). https://doi.org/10.1007/s10086-006-0827-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10086-006-0827-y

Key words