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Official Journal of the Japan Wood Research Society

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Transmission of mitochondrial plasmids in protoplast cell fusion between compatible monokaryons of Lentinula edodes

Journal of Wood Science volume 53, pages 264–267 (2007)Cite this article

Abstract

Electrophoretic analysis of the transmission pattern of mitochondrial plasmids in protoplast cell fusion between compatible monokaryons of Lentinula edodes indicates that three of the four plasmids carried in parental monokaryons are effectively transferred and replicated in the protoplast fusants. The two monokaryons, 1158a and 1569a, carried different plasmids that could be distinguished by a single restriction digest. Electrophoresis of intact plasmids and restriction analyses indicate that all but one of the fusants carry three of the four possible plasmids, indicating that transmission of plasmids in protoplast fusions is principally biparental in L. edodes. Thus, heterocytoplasmic cells of L. edodes can be effectively constructed by protoplast cell fusion. In addition, plasmids of the same homology group cannot coexist in the heteroplasmic cells after protoplast cell fusion.

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Authors and Affiliations

  1. Faculty of Agriculture, Shinshu University, Minamiminowa, Nagano, 399-4598, Japan

    Masaki Fukuda, Izumi Sendo & Satoko Imahori

  2. Tottori Mycological Institute, Tottori, 689-1125, Japan

    Yukitaka Fukumasa-Nakai

Authors
  1. Masaki Fukuda
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  2. Izumi Sendo
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  3. Satoko Imahori
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  4. Yukitaka Fukumasa-Nakai
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Corresponding author

Correspondence to Masaki Fukuda.

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Contribution No. 382 of the Tottori Mycological Institute

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Fukuda, M., Sendo, I., Imahori, S. et al. Transmission of mitochondrial plasmids in protoplast cell fusion between compatible monokaryons of Lentinula edodes . J Wood Sci 53, 264–267 (2007). https://doi.org/10.1007/s10086-006-0843-y

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  • DOI: https://doi.org/10.1007/s10086-006-0843-y

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