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Journal of Wood Science

Official Journal of the Japan Wood Research Society

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Antioxidant activity of extracts from the wood and bark of Port Orford cedar

Journal of Wood Science volume 53, pages 147–152 (2007)Cite this article

Abstract

Heartwood, sapwood, and inner and outer bark of Port Orford cedar were extracted with methanol, and the extracts evaluated for antioxidant activity. The total phenol content (TPC) of the extracts was determined by the Folin-Ciocalteu method and expressed as gallic acid equivalent (GAE). Butylated hydroxytoluene was used as a positive control in the free-radical-scavenging activity tests and ethylenediaminetetraacetic acid dihydrate disodium salt served as a positive control in the metal-chelating activity assay. All wood extracts showed significant freeradical-scavenging activity. In the radical-scavenging assay of 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (the ABTS assay), the inner bark extracts exhibited the strongest free-radical-scavenging activity. The 50% inhibitory concentrations (IC50) in the radical-scavenging assay against 1,1-diphenyl-2-picrylhydrazyl hydrate radical (DPPH) of the heartwood, sapwood, and inner and outer bark extracts were 64.77, 29.03, 10.31, 19.87 μg·ml−1, respectively. In the metal-chelating activity system, the sapwood extract demonstrated significant activity. The greatest TPC, 537.5 mg GAE/g dry extract, was detected in the inner bark. The lowest TPC of 136.9 mg GAE/g dry extract was observed in the heartwood dry extract. The results indicate that the antioxidant activities of the extracts are in accordance with the amounts of phenolics present; the inner and outer barks of Port Orford cedar are rich in phenolics and may provide good sources of antioxidants.

References

  1. Harlow WM, Harrar ES, White FM (1978) Textbook of dendrology. McGraw Hill, New York

    Google Scholar 

  2. Miliauskas G, Venskutonis PR, Van Beek TA (2004) Screening of radical scavenging activity of some medicinal and aromatic plant extracts. Food Chem 85:261–237

    Article  Google Scholar 

  3. Dapkevicius A, Venskutonis R, Van-Beek TA, Linssen JPH (1998) Antioxidant activity of extracts obtained by different isolation procedures from some aromatic herbs grown in Lithuania. J Sci Food Agric 77:140–146

    Article  CAS  Google Scholar 

  4. Springfield EP, Amabeoku G, Weitz F, Mabusela W, Johnson Q (2003) An assessment of two carpobrotus species extracts as potential antimicrobial agent. Phytomedicine 10:434–439

    Article  CAS  PubMed  Google Scholar 

  5. Craig MA, Karchesy JJ, Blythe LL, González-Hernánde MP, Swan LR (2004) Toxicity studies on western juniper oil (Juniperus occidentalis) and Port-Orford cedar oil (Chamaecyparis lawsoniana) extracts utilizing local lymph node and acute dermal irritation assays. Toxicol Lett 154:217–224

    Article  CAS  PubMed  Google Scholar 

  6. McDaniel CA (1989) Major termiticidal components of heartwood of Port-Orford-cedar Chamaecyparis Lawsoniana (A.Murr.) Parl. Mater Organismen 24:1–15

    CAS  Google Scholar 

  7. Morrell JJ, Sexton CM (1987) Decay resistance of Port-Orford-cedar. Forest Prod J 37:49–50

    Google Scholar 

  8. Tucker AO, Maciarello MJ, Karchesy JJ (2000) Commercial “rose of cedar” oil the wood oil of Port-Orford-cedar Chamaecyparis lawsoniana (A. Murray) Parl. (Cupressaceae). J Essen Oil Res 12:24–26

    Article  CAS  Google Scholar 

  9. Aruoma OI (1994) Nutrition and health aspects of free radical and antioxidants. Food Chem Toxicol 62:671–683

    Article  Google Scholar 

  10. Pietta DG (2000) Flavonoids as antioxidants. J Nat Prod 63:1035–1043

    Article  CAS  PubMed  Google Scholar 

  11. Frei B (1995) Cardiovascular disease and nutrient antioxidants: role of low-density lipoprotein oxidation. Crit Rev Food Sci Nutr 35:83–98

    Article  CAS  PubMed  Google Scholar 

  12. Grice HC (1986) Safety evaluation of butylated hydroxyltoluene (BHT) in the liver, lung and gastrointestinal tract. Food Chem Toxicol 24:1127–1130

    Article  Google Scholar 

  13. Wichi HP (1998) Enhanced tumor development by butylated hydroxyanisole (BHA) from the prospective of effect on forestomach and oesophageal squamous epithelium. Food Chem Toxicol 26:717–723

    Google Scholar 

  14. Williams GM, Iatropoulos MJ, Whysner J (1999) Safety assessment of butylated hydroxyanisole and butylated hydroxyltoluene as antioxidant food additives. Food Chem Toxicol 37:1027–1038

    Article  CAS  PubMed  Google Scholar 

  15. Flournoy DS, Paul JA, Kirk K, Highley TL (1993) Changes in the size and volume of pores in sweetgum wood during simultaneous rot by Phanerochaete chrysosporium Burds. Holzforschung 47:297–301

    Article  CAS  Google Scholar 

  16. Backa S, Gierer J, Reitberger T, Nilsson T (1992) Hydroxyl radical activity in brown-rot fungi studied by a new chemiluminescence method. Holzforschung 46:61–67

    Article  CAS  Google Scholar 

  17. Backa S, Gierer J, Reitberger T, Nilsson T (1993) Hydroxyl radical activity associated with the growth of white-rot fungi. Holzforschung 47:181–187

    Article  CAS  Google Scholar 

  18. Schultz TD, Nicholas DD (2000) Naturally durable heartwood: evidence for a proposed dual defensive function of extracts. Phytochemistry 54:47–52

    Article  CAS  PubMed  Google Scholar 

  19. Schultz TD, Nicholas DD (2002) Development of environmentally benign wood preservatives based on the combination of organic biocides with antioxidants and metal chelators. Phytochemistry 61:555–560

    Article  CAS  PubMed  Google Scholar 

  20. Payet B, Shum A, Sing C, Smadja J (2005) Assessment of antioxidant activity of cane brown sugars by ABTS and DPPH radical scavenging assays: determination of their polyphenolic and volatile constituents. J Agric Food Chem 53:10074–10079

    Article  CAS  PubMed  Google Scholar 

  21. Yen GC, Duh PD, Tsai CL (1993) Relationship between antioxidant activity and maturity of peanut hulls. J Agric Food Chem 41:67–70

    Article  CAS  Google Scholar 

  22. Burits M, Bucar F (2000) Antioxidant activity of Nigella sativa essential oil. Phytother Res 14:323–328

    Article  CAS  PubMed  Google Scholar 

  23. Cuendet M, Hostettmann K, Potterat O (1997) Iridoid glucosides with free radical scavenging properties from Fragraea blumei. Helv Chim Acta 80:1144–1152

    Article  CAS  Google Scholar 

  24. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C (1999) Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 26:1231–1237

    Article  CAS  PubMed  Google Scholar 

  25. Dinis TCP, Madeita VMC, Almeida LM (1994) Action of phenolic derivatives (acetaminophen, salicylate, and 5-aminosalicylate) as inhibitors of membrane lipid peroxidation and as peroxylradical scavengers. Arch Biochem Biophys 315:161–169

    Article  CAS  PubMed  Google Scholar 

  26. Singleton VL, Orthofer R, Lamuela-Raventós (1999) Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods Enzymol 299:152–178

    Article  CAS  Google Scholar 

  27. Soler-Rivas C, Espín JC, Wichers HJ (2000) An easy and fast test to compare total free radical scavenger capacity of foodstuffs. Photochem Anal 11:330–338

    Article  CAS  Google Scholar 

  28. Yamaguchi F, Ariga T, Yoshimura Y, Nakazawa H (2000) Antioxidative and anti-glycation activity of garcinol from Garcinia indica fruit rind. J Agric Food Chem 48:180–185

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Louisiana Forest Products Development Center, School of Renewable of Natural Resources, Louisiana State University AgCenter, Baton Rouge, LA, 70803, USA

    Heng Gao & Todd F. Shupe

  2. USDA Forest Service, Southern Research Station, Pineville, LA, 71360, USA

    Thomas L. Eberhardt & Chung Y. Hse

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  1. Heng Gao
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  2. Todd F. Shupe
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  3. Thomas L. Eberhardt
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  4. Chung Y. Hse
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Correspondence to Todd F. Shupe.

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Gao, H., Shupe, T.F., Eberhardt, T.L. et al. Antioxidant activity of extracts from the wood and bark of Port Orford cedar. J Wood Sci 53, 147–152 (2007). https://doi.org/10.1007/s10086-006-0850-z

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  • DOI: https://doi.org/10.1007/s10086-006-0850-z

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