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Journal of Wood Science

Official Journal of the Japan Wood Research Society

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Analyses of the genetic diversity of matsutake isolates collected from different ecological environments in Asia

Journal of Wood Science volume 53, pages 344–350 (2007)Cite this article

Abstract

Matsutake mushrooms are able to grow in two kinds of ecological environments: coniferous forests and broadleaf forests. Japanese matsutake is a form of the matsutake mushroom that mainly appears in coniferous forests in the mid-latitude regions of Asia. In contrast, matsutake from Yunnan and Sichuan Provinces of China grows in broadleaf forests in low-latitude regions. The taxonomic relationship and the genetic distance between these two types of matsutake remain unclear. Here, we compared the random amplification of polymorphic DNA (RAPD) data and the sequences of the internal transcribed spacer (ITS) region and of variable domains V4, V6, and V9 of mitochondrial small subunit ribosomal DNA (mt SSU rDNA) to determine the genetic differences of Tricholoma matsutake strains collected from different ecological and geographic environments in Asia. We found few differences in the sequences of the variable domains, implying that all matsutake strains are the same biological species regardless of their host specificity and geographical differences. This conclusion is also supported by the sequences of the ITS region. On the other hand, RAPD analysis revealed that the matsutake mushrooms collected from different ecological environments have genetic differences and represent distinct varieties.

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Authors and Affiliations

  1. Laboratory of Microbial Biotechnology, Faculty of Agriculture, Tottori University, Tottori, 680-8553, Japan

    Dapeng Bao, Ayumi Koike & Tadanori Aimi

  2. Institute of Mycology, Jilin Agricultural University, Changchun City, Jilin Province, 130118, P. R. China

    Fangjie Yao

  3. Kyoto Mycological Institute, Misasahirabayashi, Yamashina, Kyoto, 607-8406, Japan

    Katsuji Yamanaka

  4. Asano Life Science Laboratory, Asano Industry Co. Ltd., 3-20-6 Tamahara, Tamano, 706-0014, Japan

    Yutaka Kitamoto

Authors
  1. Dapeng Bao
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  2. Ayumi Koike
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  3. Fangjie Yao
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  4. Katsuji Yamanaka
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  5. Tadanori Aimi
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  6. Yutaka Kitamoto
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Correspondence to Yutaka Kitamoto.

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Bao, D., Koike, A., Yao, F. et al. Analyses of the genetic diversity of matsutake isolates collected from different ecological environments in Asia. J Wood Sci 53, 344–350 (2007). https://doi.org/10.1007/s10086-006-0859-3

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  • DOI: https://doi.org/10.1007/s10086-006-0859-3

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