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Journal of Wood Science

Official Journal of the Japan Wood Research Society

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Introduction of mitochondrial DNA from Pleurotus ostreatus into Pleurotus pulmonarius by interspecific protoplast fusion

Journal of Wood Science volume 53, pages 339–343 (2007)Cite this article

Abstract

Interspecific protoplast fusion between two monokaryotic strains (a methionine auxotrophic and chloramphenicol-resistant Pleurotus ostreatus strain and a wild-type strain of Pleurotus pulmonarius) was carried out to introduce mitochondrial DNA (mtDNA) from P. ostreatus into P. pulmonarius. Because mycelial colonies regenerated on minimum medium containing chloramphenicol only after the treatment of protoplast electrofusion, the regenerants were regarded as protoplast fusants. The fusants isolated from regenerated colonies were uninucleate, and their resistance of chloramphenicol seemed to be a stable trait. The fusants mated with P. pulmonarius but not with P. ostreatus, and showed almost identical random amplified polymorphic DNA (RAPD) patterns to that of the parental P. pulmonarius monokaryon. The sizes of mitochondrial genomes were estimated to be 81.5 kb for P. ostreatus monokaryon, 54.9 kb for P. pulmonarius monokaryon, and 84.5 or 86.0 kb for the fusants. BamHI and EcoRI digest patterns of the fusant mtDNAs were almost the same as the parental P. ostreatus monokaryon. From the above results, the fusants seemed to carry nuclei derived from the monokaryon of P. pulmonarius and mtDNA including the chloramphenicol-resistance gene from the P. ostreatus monokaryon, suggesting that the P. ostreatus mtDNA had been introduced into P. pulmonarius cells by interspecific protoplast fusion.

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Authors and Affiliations

  1. Faculty of Agriculture, Shinshu University, 8304 Minamiminowa, Nagano, 399-4598, Japan

    Masaki Fukuda, Masayoshi Wakayama & Masayuki Uchida

  2. Tottori Mycological Institute, Tottori, 689-1125, Japan

    Yukitaka Fukumasa-Nakai & Teruyuki Matsumoto

Authors
  1. Masaki Fukuda
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  2. Masayoshi Wakayama
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  3. Masayuki Uchida
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  4. Yukitaka Fukumasa-Nakai
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  5. Teruyuki Matsumoto
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Correspondence to Masaki Fukuda.

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Contribution No. 383 of the Tottori Mycological Institute

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Fukuda, M., Wakayama, M., Uchida, M. et al. Introduction of mitochondrial DNA from Pleurotus ostreatus into Pleurotus pulmonarius by interspecific protoplast fusion. J Wood Sci 53, 339–343 (2007). https://doi.org/10.1007/s10086-006-0861-9

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  • DOI: https://doi.org/10.1007/s10086-006-0861-9

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