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Journal of Wood Science

Official Journal of the Japan Wood Research Society

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Excitation wavelength-specific changes in lignocellulosic autofluorescence

Journal of Wood Science volume 53, pages 358–360 (2007)Cite this article

Abstract

Lignocellulose (paper sheets of thermomechanical pulp) was irradiated with specific wavelengths in an epifluorescence microscope equipped with filter cubes with excitation and emission filters commonly used for excitation of fluorophores with blue, green, and red emissions. The irradiation was shown to affect the relative autofluorescence intensity over time. Shorter wavelength excitation (λex = 340–380 nm) induced photoyellowing that increased the autofluorescence. Filter cubes allowing irradiation at longer wavelengths (λex = 450–490 nm and λex = 515–560, respectively) caused photobleaching and a decrease in lignocellulosic autofluorescence. The increase and decrease in autofluorescence was reversible; prephotoyellowed samples could be photobleached and prephotobleached samples regained autofluorescence by shorter wavelength irradiation. Thus, the specificity of the excitation wavelength and the time-dependent autofluorescence might affect long-term imaging experiments of lignocellulose-based samples.

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  1. Department of Wood Science, Swedish University of Agricultural Sciences, Wood Ultrastructure Research Centre (WURC), Box 7008, Uppsala, SE-750 07, Sweden

    Jonas Hafrén

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  1. Jonas Hafrén
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Correspondence to Jonas Hafrén.

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Hafrén, J. Excitation wavelength-specific changes in lignocellulosic autofluorescence. J Wood Sci 53, 358–360 (2007). https://doi.org/10.1007/s10086-006-0862-8

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  • DOI: https://doi.org/10.1007/s10086-006-0862-8

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