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Journal of Wood Science

Official Journal of the Japan Wood Research Society

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Selective staining and visualization of hyphal sheath of a white-rot fungus Phanerochaete crassa WD1694 with phloxine B

Journal of Wood Science volume 54, pages 76–80 (2008)Cite this article

Abstract

The hyphal sheath is a morphological feature of many kinds of fungi. Although the fine structures of the sheath have been studied in detail by a number of electron microscopy techniques, the function and physiology of the hyphal sheath are not yet clarified. One reason for this is that the hyphal sheath is a colorless, mucilaginous, and delicate material so that it is not easily identified. We developed a simple method to visualize and identify the hyphal sheath of the white-rot fungus Phanerochaete crassa WD1694. The small mycelial pellets in shaken liquid cultures of P. crassa WD1694 were stained directly with phloxine B. Both the hyphae and the hyphal sheath that filled the gaps between each of the hyphae were visualized and observed by light microscopy. The stained hyphae were further studied by transmission electron microscopy, atomic force microscopy, and fl uorescence microscopy. Based on these observations, we confirmed that the staining of the hyphae was also due to the presence of the hyphal sheath that closely covered the fungal cell wall. These results clearly showed that the hyphal sheath was selectively stained with phloxine B and could be observed and identified by conventional light microscopy.

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Authors and Affiliations

  1. Forestry and Forest Products Research Institute, 1 Matsunosato, Tsukuba, Ibaraki, 305-8687, Japan

    Mariko Takano, Noriko Hayashi & Katsushi Kuroda

Authors
  1. Mariko Takano
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  2. Noriko Hayashi
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  3. Katsushi Kuroda
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Correspondence to Mariko Takano.

Additional information

Part of this report was presented at the 50th Lignin Symposium, Nagoya, October 2005

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Takano, M., Hayashi, N. & Kuroda, K. Selective staining and visualization of hyphal sheath of a white-rot fungus Phanerochaete crassa WD1694 with phloxine B. J Wood Sci 54, 76–80 (2008). https://doi.org/10.1007/s10086-007-0904-x

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  • DOI: https://doi.org/10.1007/s10086-007-0904-x

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