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Official Journal of the Japan Wood Research Society

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Preparation and crossing of mating-capable monokaryons via protoplasting of the dikaryotic mycelia of a mycorrhizal mushroom, Lyophyllum shimeji

Journal of Wood Science volume 54, pages 337–340 (2008)Cite this article

Abstract

In order to develop a novel method of obtaining monokaryons for a mycorrhizal fungus, Lyophyllum shimeji, monokaryotization of dikaryotic stock culture via protoplast formation and regeneration was performed using 12 dikaryotic stocks. From 6 dikaryotic stocks, a total of 120 monokaryons were isolated, and their mating compatibility was tested. Mating-compatible monokaryons were successfully derived from a dikaryotic stock (NBRC 100325), and monokaryons of only 1 mating type relative to the parental dikaryons were isolated from another 3 strains (MH01710, OK2L-1, and HY7L-1). We successfully prepared monokaryotic stocks via protoplast monokaryotization, a technique that can be used to identify biological species of L. shimeji. This technique could be used for breeding various mycorrhizal mushrooms, including Tricholoma matsutake, for which the preparation of monospore cultures is extremely difficult.

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Authors and Affiliations

  1. The United Graduate School of Agricultural Sciences, Tottori University, Tottori, 680-8553, Japan

    Kazuhiko Maeta

  2. Scientific Crime Investigation Laboratory, Tottori Police Headquarters, Tottori, 680-0911, Japan

    Kazuhiko Maeta

  3. Faculty of Agriculture, Tottori University, 4-101 Koyama-cho Minami, Tottori, 680-8553, Japan

    Ayumi Koike, Hiroyuki Mukaiyama, Tomoya Ochi & Tadanori Aimi

  4. School of Agriculture, Kin-ki University, Nara, 631-8505, Japan

    Takao Terashita

  5. Science Laboratory, Asano Industry Co. Ltd., Tamano, Okayama, 706-0014, Japan

    Yutaka Kitamoto

Authors
  1. Kazuhiko Maeta
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  2. Ayumi Koike
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  3. Hiroyuki Mukaiyama
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  4. Tomoya Ochi
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  5. Takao Terashita
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  6. Yutaka Kitamoto
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  7. Tadanori Aimi
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Corresponding author

Correspondence to Tadanori Aimi.

Additional information

Part of this study was presented at the 11th Annual Meeting of the Japanese Society of Mushroom Science and Biotechnology (Asahikawa), September 2007

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Maeta, K., Koike, A., Mukaiyama, H. et al. Preparation and crossing of mating-capable monokaryons via protoplasting of the dikaryotic mycelia of a mycorrhizal mushroom, Lyophyllum shimeji . J Wood Sci 54, 337–340 (2008). https://doi.org/10.1007/s10086-008-0949-5

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  • DOI: https://doi.org/10.1007/s10086-008-0949-5

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