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Journal of Wood Science

Official Journal of the Japan Wood Research Society

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At5g54160 gene encodes Arabidopsis thaliana 5-hydroxyconiferaldehyde O-methyltransferase

Journal of Wood Science volume 54, pages 312–317 (2008)Cite this article

Abstract

The function of an Arabidopsis thaliana gene, At5g54160 annotated as a caffeic acid O-methyltransferase CAOMT gene was characterized. The recombinant enzyme of this gene (AtOMT1) catalyzed the O-methylation of phenylpropanoid and flavonoid substrates. The specificity constants (k cat/K m) for 5-hydroxyconiferaldehyde (5-HCAld) and quercetin were both 0.11 μM−1·min−1. On the other hand, lignins of At5g54160-knockout Arabidopsis mutants lacked syringyl units. In addition, we showed that the gene silencing also resulted in significant accumulation of caffeyl alcohol (CaAlc). These results strongly suggested that At5g54160 gene is involved in syringyl lignin synthesis for the methylation of both 5-hydroxyconiferaldehyde and 3,4-dihydroxyphenyl compound(s).

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Author notes

  1. Yu Kitamura

    Present address: Nippon Paper Industries Co., Ltd., Iwakuni, 740-0003, Japan

  2. Norikazu Sakakibara

    Present address: Faculty of Pharmaceutical Sciences at Kagawa Campus, Tokushima Bunri University, Sanuki, 769-2193, Japan

Authors and Affiliations

  1. Research Institute for Sustainable Humanosphere, Kyoto University, Gokasho, Uji, Kyoto, 611-0011, Japan

    Tomoyuki Nakatsubo, Yu Kitamura, Norikazu Sakakibara, Takefumi Hattori & Toshiaki Umezawa

  2. Institute for Chemical Research, Kyoto University, Uji, 611-0011, Japan

    Masaharu Mizutani

  3. Institute of Sustainability Science, Kyoto University, Uji, 611-0011, Japan

    Shiro Suzuki & Toshiaki Umezawa

  4. Kazusa DNA Research Institute, Kisarazu, 292-0818, Japan

    Nozomu Sakurai & Daisuke Shibata

Authors
  1. Tomoyuki Nakatsubo
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  2. Yu Kitamura
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  3. Norikazu Sakakibara
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  4. Masaharu Mizutani
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  5. Takefumi Hattori
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  6. Nozomu Sakurai
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  7. Daisuke Shibata
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  8. Shiro Suzuki
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  9. Toshiaki Umezawa
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Corresponding author

Correspondence to Toshiaki Umezawa.

Additional information

Part of this work was presented at the Annual Meeting of Japan Society for Bioscience, Biotechnology, and Agrochemistry, March 24–27, 2007

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Nakatsubo, T., Kitamura, Y., Sakakibara, N. et al. At5g54160 gene encodes Arabidopsis thaliana 5-hydroxyconiferaldehyde O-methyltransferase. J Wood Sci 54, 312–317 (2008). https://doi.org/10.1007/s10086-008-0958-4

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  • DOI: https://doi.org/10.1007/s10086-008-0958-4

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