Skip to main content
Journal of Wood Science

Official Journal of the Japan Wood Research Society

Download PDF
  • Note
  • Published:

Morphological changes in the cytoskeleton, nuclei, and vacuoles during cell death of short-lived ray tracheids in the conifer Pinus densiflora

Journal of Wood Science volume 54, pages 509–514 (2008)Cite this article

Abstract

Morphological changes in the cytoskeleton, nuclei, and vacuoles were monitored during the cell death of short-lived ray tracheids in the conifer Pinus densiflora. After formation of the dentate thickenings that occurred at the final stage of formation of cell walls, organelles started to disappear in differentiating ray tracheids. First, the microtubules and vacuoles disappeared. Then actin filaments disappeared in the differentiating ray tracheids adjacent to ray tracheids that lacked nuclei, and, finally, the nuclei disappeared. These features indicate that cell death in ray tracheids might differ from the programmed cell death of tracheary elements that has been studied in vitro in the Zinnia culture system.

References

  1. Funada R (2008) Mechanism of regulation of cambial activity in trees (in Japanese). Mokuzai Gakkaishi 54:1–10

    Article  CAS  Google Scholar 

  2. Nakaba S, Sano Y, Kubo T, Funada R (2006) The positional distribution of cell death of ray parenchyma in a conifer, Abies sachalinensis. Plant Cell Rep 25:1143–1148

    Article  CAS  PubMed  Google Scholar 

  3. Fukuda H (1997) Tracheary element differentiation. Plant Cell 9:1147–1156

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Fukuda H (2004) Signals that control plant vascular cell differentiation. Nat Rev Mol Cell Biol 5:379–391

    Article  CAS  PubMed  Google Scholar 

  5. Imagawa H (1984) Study on the ray formation in Japanese larch (in Japanese). Res Bull Coll Exp Forest Hokkaido Univ 42:133–148

    Google Scholar 

  6. Nakaba S, Kubo T, Funada R (2008) Differences in patterns of cell death between ray parenchyma cells and ray tracheids in the conifers Pinus densiflora and Pinus rigida. Trees. doi 10.1007/s00468-008-0220-0

  7. Jones AM (2001) Programmed cell death in development and defense. Plant Physiol 125:94–97

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Kuriyama H, Fukuda H (2002) Developmental programmed cell death in plants. Curr Opin Plant Biol 5:568–573

    Article  CAS  PubMed  Google Scholar 

  9. Higaki T, Goh T, Hayashi T, Kutsuna N, Kadota Y, Hasezawa S, Sano T, Kuchitsu K (2007) Elicitor-induced cytoskeletal rearrangement relates to vacuolar dynamics and execution of cell death: in vivo imaging of hypersensitive cell death in tobacco BY-2 cells. Plant Cell Physiol 48:1414–1425

    Article  CAS  PubMed  Google Scholar 

  10. Smertenko AP, Bozhkov PV, Filonova LH, von Arnold S, Hussey PJ (2003) Re-organisation of the cytoskeleton during developmental programmed cell death in Picea abies embryos. Plant J 33:813–824

    Article  CAS  PubMed  Google Scholar 

  11. Doorn WG, Woltering EJ (2005) Many ways to exit? Cell death categories in plants. Trends Plant Sci 10:117–122

    Article  PubMed  Google Scholar 

  12. Sonobe S, Shibaoka H (1989) Cortical fine actin filaments in higher plant cells visualized by rhodamine-phalloidin after pretreatment with m-maleimidobenzoyl N-hydroxysuccinimide ester. Protoplasma 148:80–86

    Article  Google Scholar 

  13. Funada R (2002) Immunolocalisation and visualisation of the cytoskeleton in gymnosperms using confocal laser scanning microscopy (CLSM). In: Chaffey N (ed) Wood formation in trees: cell and molecular biology techniques. Taylor and Francis, London, pp 143–157

    Chapter  Google Scholar 

  14. Abe H, Funada R, Imaizumi H, Ohtani J, Fukazawa K (1995) Dynamic changes in the arrangement of cortical microtubules in conifer tracheids during differentiation. Planta 197:418–421

    Article  CAS  Google Scholar 

  15. Abe H, Funada R, Ohtani J, Fukazawa K (1995) Changes in the arrangement of microtubules and microfibrils in differentiating conifer tracheids during the expansion of cells. Ann Bot 75: 305–310

    Article  Google Scholar 

  16. Rotman BB, Papermaster BW (1966) Membrane properties of living mammalian cells as studied by hydrolysis of fluorogenic esters. Proc Nat Acad Sci USA 55:134–141

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. Rensing KH, Samuels AL (2004) Cellular changes associated with rest and quiescence in winter-dormant vascular cambium of Pinus contorta. Trees 18:373–380

    Article  Google Scholar 

  18. Funada R (2000) Control of wood structure. In: Nick P (ed) Plant microtubules: potential for biotechnology. Springer, Berlin Heidelberg New York, pp 51–81

    Chapter  Google Scholar 

  19. Funada R (2008) Microtubules and the control of wood formation. In: Nick P (ed) Plant microtubules: development and flexibility. Springer, Berlin Heidelberg New York, pp 83–119

    Chapter  Google Scholar 

  20. Chaffey N, Barlow P (2002) Myosin, microtubules, and microfilaments: co-operation between cytoskeletal components during cambial cell division and secondary vascular differentiation in trees. Planta 214:526–536

    Article  CAS  PubMed  Google Scholar 

  21. Funada R, Abe H, Furusawa O, Imaizumi H, Fukazawa K, Ohtani J (1997) The orientation and localization of cortical microtubules in differentiating conifer tracheids during cell expansion. Plant Cell Physiol 38:210–212

    Article  CAS  Google Scholar 

  22. Funada R, Miura H, Shibagaki M, Furusawa O, Miura T, Fukatsu E, Kitin P (2001) Involvement of localized cortical microtubules in the formation of a modified structure of wood. J Plant Res 114: 491–497

    Article  Google Scholar 

  23. Chaffey N, Barlow P, Barnett J (2000) A cytoskeletal basis for wood formation in angiosperm trees: the involvement of microfilaments. Planta 210:890–896

    Article  CAS  PubMed  Google Scholar 

  24. Chaffey N, Barlow P, Sundberg B (2002) Understanding the role of the cytoskeleton in wood formation in angiosperm trees: hybrid aspen (Populus tremula × P. tremuloides) as the model species. Tree Physiol 22:239–249

    Article  CAS  PubMed  Google Scholar 

  25. Furusawa O, Funada R, Murakami Y, O htani J (1998) Arrangement of cortical microtubules in compression wood tracheids of Taxus cuspidata visualized by confocal laser microscopy. J Wood Sci 44:230–233

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, Tokyo, 183-8509, Japan

    Satoshi Nakaba, Joto Yoshimoto, Takafumi Kubo & Ryo Funada

Authors
  1. Satoshi Nakaba
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Joto Yoshimoto
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Takafumi Kubo
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ryo Funada
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ryo Funada.

Additional information

This study was presented at the 57th Annual Meeting of the Japan Wood Research Society, August 8–10, 2007, Hiroshima, Japan

Rights and permissions

Reprints and permissions

About this article

Cite this article

Nakaba, S., Yoshimoto, J., Kubo, T. et al. Morphological changes in the cytoskeleton, nuclei, and vacuoles during cell death of short-lived ray tracheids in the conifer Pinus densiflora . J Wood Sci 54, 509–514 (2008). https://doi.org/10.1007/s10086-008-0971-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10086-008-0971-7

Key words