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Official Journal of the Japan Wood Research Society

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Extractives of Quercus crispula sapwood infected by the pathogenic fungi Raffaelea quercivora I: comparison of sapwood extractives from noninfected and infected samples

Journal of Wood Science volume 55, pages 126–132 (2009)Cite this article

A Correction to this article was published on 27 April 2018

Abstract

The extracts of Quercus crispula infected by the ambrosia fungus, Raffaelea quercivora, were investigated. Phenol and tannin analyses indicated that normal sapwood (NS) contained a considerable amount of hydrolysable tannins, while infected colored sapwood (IS) contained less hydrolysable tannins and more phenols than NS. In treating pentagalloyl glucose (PGG), which is a model compound of hydrolysable tannins, with a culture medium of R. quercivora, PGG was rapidly hydrolyzed to produce gallic acid. The resulting gallic acid decreased in concentration over the subsequent cultivation period eventually disappeared. Measuring tannase and laccase activities of the culture medium of R. quercivora, tannase activity increased gradually from the beginning, while laccase activity increased rapidly at 5 days of incubation and disappeared at 8 days. An oxidative product from gallic acid treated with laccase was isolated by preparative high performance liquid chromatography, and was identified as purprogallincarboxylic acid (PGCA) by nuclear magnetic resonance spectroscopy and electron-impact mass spectrometry. PGCA was present in a 70% aqueous acetone extract of IS, and showed slight growth inhibition against R. quercivora.

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Authors and Affiliations

  1. Department of Applied Life Science, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu, 510-1193, Japan

    Kayoko Imai, Tohru Mitsunaga & Hideo Ohashi

  2. Center for Ecological Research, Kyoto University, Otsu, Shiga, 520-2113, Japan

    Hiroyuki Takemoto

  3. Experimental Section at Chiba, The University Forest, The University of Tokyo, Kamogawa, Chiba, 299-5503, Japan

    Toshihiro Yamada

  4. Department of Sustainable Resource Sciences, Faculty of Bioresources, Mie University, Tsu, Mie, 514-8507, Japan

    Shin-ichiro Ito

Authors
  1. Kayoko Imai
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  2. Tohru Mitsunaga
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  3. Hiroyuki Takemoto
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  4. Toshihiro Yamada
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  5. Shin-ichiro Ito
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  6. Hideo Ohashi
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Corresponding author

Correspondence to Tohru Mitsunaga.

Additional information

Part of this study was presented at the 57th Annual Meeting of the Japan Wood Research Society, Hiroshima, Japan, 2007

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Imai, K., Mitsunaga, T., Takemoto, H. et al. Extractives of Quercus crispula sapwood infected by the pathogenic fungi Raffaelea quercivora I: comparison of sapwood extractives from noninfected and infected samples. J Wood Sci 55, 126–132 (2009). https://doi.org/10.1007/s10086-008-1005-1

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  • DOI: https://doi.org/10.1007/s10086-008-1005-1

Key words

  • Raffaelea quercivora
  • Quercus crispula
  • Gallotannins
  • Ellagitannins
  • Tannase